Somatic Embryogenesis in Strawberry : Induction , control and application of somatic embryogenesis in strawberry

ISBN-13: 9783330841666 / Angielski / Miękka / 2016 / 236 str.

Genesia Omar; Fouad Mohamed; Klaus Haench

Opis

This study was conducted to examine the possibility of inducing somatic embryogenesis in strawberry using tissue culture technique. Experimental works included evaluation of five published protocols which did not prove the induction of somatic embryogenesis by histological means, in addition to examine, alternative approaches. The embryogenic nature of the regenerating tissues was checked by histological analysis. Factors regarding the plant materials (cultivars and explant source) and those concernal with external effects (medium component, light and temperature) were studied. Results of repeating published protocols indicated the formation of callus and shoot-like structures (protocol of Wang et al, 1984), or somatic embryo-like structures (SELS) and shoot buds (protocols of Donnoli et al, 2001 and Biswas et al,2007). Protocol (4) of Husaini et al(2008) also produced callus, SELS or direct shoot regeneration, while protocol 5 (Kordestani and Karami, 2008) resulted in only callus. Morphohistological examination did not prove the induction of somatic embryo in the five tested protocols. The process of somatic embryogenesis in strawberry was further studied utilizing different combi

O autorze

Dr. Genesia Omar, currently I am lectuer in plant biotechnology of Horticulture department, Faculty of Agriculture, Suez Canal University.I finished my doctoral research in Germany, My research papers are concentrated in improving invitro mass production of vegetable crops, Also I Teach practical lessons in the field of biotechnology