Cardiomyocytes: Methods and Protocols

ISBN-13: 9781493925711 / Angielski / Twarda / 2015 / 216 str.

Gary R. Skuse; Maureen Ferran

Opis

This volume has been assembled for scientists interested in basic and applied biomedical research directed toward understanding the development, genetics and function of cardiomyocytes. The methods and protocols contained within it address cell culture techniques, cardiomyocyte differentiation and redifferentiation, experimental induction of cardiomyopathies, introducing genes into cardiomyocytes, genomic approaches to the understanding cardiomyocytes, cryopreservation of neonatal cardiomyocytes and modeling of cardiomyocyte function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Practical and current, Cardiomyocytes: Methods and Protocols explores complimentary areas of cardiomyocyte science that, taken together, can inform individuals with a broad range of interests.

Spis treści

1. Generating Primary Cultures of Murine Cardiac Myocytes and Cardiac Fibroblasts to Study Viral Myocarditis

            Barbara Sherry

 

2. Enrichment of Cardiomyocytes in Primary Cultures of Murine Neonatal Hearts

            Sreejit Parameswaran, Rajalakshmi Santhakumar, Prasanna Vidyasekar, and Rama S. Verma

 

3. Deep Sequencing of Cardiac MicroRNA-mRNA Interactomes in Clinical and Experimental Cardiomyopathy

            Scot J. Matkovich and Gerald W. Dorn, II

 

4. Next Generation Sequencing Technology in the Genetics of Cardiovascular Disease

            Cecilia Vecoli

 

5. Computational Cardiac Electrophysiology: Implementing Mathematical Models of Cardiomyocytes to Simulate Action Potentials of the Heart

            Michael M. Bell and Elizabeth M. Cherry

 

6. Methods of Myofibrillogenesis Modeling

            Nancy K. Drew and Anna Grosberg

 

7. Using the Mechanical Bidomain Model to Analyze the Biomechanical Behavior of Cardiomyocytes

            Bradley J. Roth

 

8. Fabrication of a Myocardial Patch with Cells Differentiated from Human Induced-Pluripotent Stem Cells

            Lei Ye, Joydeep Basu, and Jianyi Zhang

 

9. Efficient Differentiation of Cardiomyocytes from Human Pluripotent Stem Cells with Growth Factors

            Rajneesh Jha, Ren-He Xu, and Chunhui Xu

 

10. Isolation, Culturing, and Characterization of Cardiac Muscle Cells from Non-Human Primate Heart Tissue

            Steven M. Hoynowski and John W. Ludlow

 

11. Mouse Embryonic Stem Cell-Derived Cardiac Myocytes in a Cell Culture Dish

            Carley Glass, Reetu Singla, Anshu Arora, and Dinender K. Singla

 

12. Cryopreservation of Neonatal Cardiomyocytes

            Adam C. Vandergriff, M. Taylor Hensley, and Ke Cheng

 

13. Evaluation of Sarcomeric Organization in Human Pluripotent Stem Cell-Derived Cardiomyocytes

            Chrishan J.A. Ramachandra and Winston Shim

 

14. Electrotonic Coupled Metabolic Purification of Chick Cardiomyocytes

            Winston Shim, Haiyang Yu, K.P. Myu Mai Ja, Muhammad Parasuram, Kee Pah Lim, and Philip Wong

 

15. Gene Transfer into Cardiac Myocytes

            Sarah E. Lang and Margaret V. Westfall

 

16. Analysis of 4D Myocardial Wall Motion During Early Stages of Chick Heart Development

            Madeline Midgett and Sandra Rugonyi