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It has now been over a decade since isoelectric focusing became estab lished as a valuable addition to our arsenal of physical methods for separa tion of proteins and other amphoteric substances. The high resolving power of the technique and its unique ability to separate as well as concentrate have been amply demonstrated in several international symposia. The past few years have led to the wide acceptance of standardized methodologies for analytical and preparative purposes. These procedures afford rapid, reproducible separations that have led to a dramatic increase in our under standing of...

The selective combination of physical, biochemical, and immunological prin- ciples, along with new knowledge concerning the biology of cells and advance- ments in engineering and computer sciences, has made possible the emergence of highly sophisticated and powerful methods for the analysis of cells and their constituents. This series on Cell Analysis is, therefore, aiming at providing the theoretical and practical background on how these methods work and what kind of information can be obtained. Cell Analysis will cover techniques on cell separation, cell identification and classification,...

Presently, the need for methods involving separation, identification, and characterization of different kinds of cells is amply realized among immu nologists, hematologists, cell biologists, clinical pathologists, and cancer researchers. Unless cells exhibiting different functions and stages of differ entiation are separated from one another, it will be exceedingly difficult to study some of the molecular mechanisms involved in cell recognition, spe cialization, interactions, cytotoxicity, and transformation. Clinical diagno sis of diseased states and use of isolated cells for therapeutic (e....