Animal Models in Diabetes Research

ISBN-13: 9781493962563 / Angielski / Miękka / 2016 / 325 str.

Hans-Georg Joost; Hadi Al-Hasani; Annette Schurmann

Opis

Detailing the latest protocols for researching animal models of diabetes, especially resistant groups of rodents such as the NOD mouse, and including expert advice on implementation, this is a valuable new volume in the Methods in Molecular Biology series.

Spis treści

Part I: Rodent Models of Type 1 Diabetes

1. The Non-Obese Diabetic (NOD) Mouse as a Model of Human Type 1 Diabetes

            Kritika Kachapati, David Adams, Kyle Bednar, and William M. Ridgway

2. Assessment of Diabetic Nephropathy in the Akita Mouse

            Jae-Hyung Chang and Susan B. Gurley

3. The BB Rat as a Model of Human Type 1 Diabetes

            Rita Bortell and Chaoxing Yang

Part II: Rodent Models of Type 2 Diabetes

4. Diabetes in Mice with Monogenic Obesity: The db/db Mouse and Its Use in the Study of Cardiac Consequences

            Darrell D. Belke and David L. Severson

5. Pathophysiology and Genetics of Obesity and Diabetes in the New Zealand Obese Mouse: A Model of the Human Metabolic Syndrome

            Reinhart Kluge, Stephan Scherneck, Annette Schürmann, and Hans-Georg Joost

6. The TALLYHO Mouse as a Model of Human Type 2 Diabetes

            Jung Han Kim and Arnold M. Saxton

7. Diet-Induced Diabetes in the Sand Rat (Psammomys obesus)

            Nurit Kaiser, Erol Cerasi, and Gil Leibowitz

8. Diabetes in Zucker Diabetic Fatty Rat

            Masakazu Shiota and Richard L. Printz

9. The GK Rat: A Prototype for the Study of Non-Overweight Type 2 Diabetes?

            Bernard Portha, Marie-Hélène Giroix, Cecile Tourrel-Cuzin, Hervé Le-Stunff, and Jamileh Movassat

10. Experimentally Induced Rodent Models of Type 2 Diabetes

            Md. Shahidul Islam and Rachel Dorothy Wilson

Part III: Other Species

 

11. Investigation and Treatment of Type 2 Diabetes in Nonhuman Primates

            Barbara C. Hansen

Part IV: General Methodology

12. Determination of Beta-Cell Function: Insulin Secretion of Isolated Islets

            Michael Willenborg, Kirstin Schumacher, and Ingo Rustenbeck

13. Determination of Beta-Cell Function: Ion Channel Function in Beta Cells

            Martina Düfer

14. Measurement of Glucose Homeostasis  In Vivo: Glucose and Insulin Tolerance Tests

            Francesco Beguinot and Cecilia Nigro

15. Measurement of Glucose Homeostasis  In Vivo: Combination of Tracers and Clamp Techniques

            Masakazu Shiota

16. Measurement of Insulin Sensitivity in Skeletal Muscle In Vitro

            Henrike Sell, Jørgen Jensen, and Juergen Eckel

17. Beta-Cell Autoimmunity

            Yannick F. Fuchs, Kerstin Adler, and Ezio Bonifacio

18. Positional Cloning of Diabetes Genes

            Gudrun A. Brockmann and Christina Neuschl

19. Retinal Digest Preparation: A Method to Study Diabetic Retinopathy

            Nadine Dietrich and Hans-Peter Hammes

20. Lineage Tracing of Pancreatic Stem Cells and Beta Cell Regeneration

            Isabelle Houbracken, Iris Mathijs, and Luc Bouwens

21. Genetic Lineage Tracing of Beta Cell Neogenesis

            Iris Mathijs, Isabelle Houbracken, and Luc Bouwens

            Rita Bortell and Chaoxing Yang

Blurb

In recent years, human studies have made enormous contributions towards an understanding of the genetic basis of diabetes mellitus; however, most of the experimentation needed for the invention and testing of novel therapeutic approaches cannot be performed in humans.  Thus, there is no alternative to appropriate animal models.  In Animal Models in Diabetes Research, expert researchers explore the current status of the most important models and procedures in order to provide a timely resource in experimental diabetology.  The first half of the volume serves as a comprehensive overview on our current knowledge of the pathogenesis and pathophysiology of diabetes in animal models through a series of reviews in model strains.  The book then continues with vital, established protocols that are employed in the characterization and study of animal models of diabetes.  As a volume in the highly successful Methods in Molecular Biology™ series, this work contains the type of detailed description and key implementation advice necessary to achieve successful results.

 

Authoritative and cutting-edge, Animal Models in Diabetes Research delivers essential content that will be an important resource to advance diabetes research in the years to come.