Xylanases of Fungi from Extreme Habitats in Egypt

ISBN-13: 9786200250018 / Angielski

Osama Abdel-Hafeez Mohamed Al-Bedak; Abdelaal Hassan Moubasher; Mady Ahmed Ismail

Opis

In this book, the following methods and techniques are described and illustrated in details: 1- The methods for chemical analyses of different water, mud and soil samples. 2- Isolation of mesophilic, halotolerant/halophilic and thermotolerant/thermophilic fungi from air, water, mud and soil. 3- Extraction of xylan from different sources and its assay by UV-spectroscopy and HPLC. 4- Screening the potential of different fungi to produce xylanases. 5- Optimization of nutritional and environmental factors for the maximization of xylanases production. 6- Different techniques of xylanases production such as solid-state and submerged fermentation. 7- Determination of relative and specific activities as well as the total protein for carboxy methyl cellulase, avicellase and xylanase enzymes. 8- Purification processes of xylanase enzyme such as: ammonium sulfate precipitation, dialysis, ion-exchange column chromatography and gel filtration column chromatography. 9- Characterization of xylanase enzyme.

O autorze

Al-Bedak, Osama Abdel-Hafeez Mohamed
Osama A. Al-Bedak PhD, Mycologist at the AUMC, Assiut University, Egypt. The author interested in Basic and Applied Mycology, Fungal Plant Pathology and Fungal Biotechnology. Member of Society of Basic and Applied Mycology (Egypt) and Member in the team work at Multidisciplinary Research Centre of Excellence,Faculty of Science, Assiut University.