Immunosenescence: Methods and Protocols

ISBN-13: 9781493929627 / Angielski / Twarda / 2015 / 270 str.

Albert C. Shaw

Opis

In this volume, expert researchers detail protocols for evaluating the protean effects of immunosenescence on innate and adaptive immune function, including approaches to the analysis of antigen receptor repertoire. In addition, Immunosenecence: Methods and Protocols guides readers through cell biology and biochemical methods for analyses of telomere dysfunction, autophagy, protein oxidation, microRNAs and DNA methylation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Immunosenecence: Methods and Protocols provides a mixture of basic and advanced protocols that will be useful for immunologists in general and investigators in aging biology in particular.

Spis treści

1 Isolation of Lipid Rafts from Human Neutrophils by Density Gradient Centrifugation

Carl Fortin and Tamas Fülöp

 

2 Flow Cytometry Analysis of NK Cell Phenotype and Function in Ageing

Raquel Tarazona, Carmen Campos, Alejandra Pera, Beatriz Sanchez-Correa and Rafael Solana

 

3 Flow Cytometric Identification of Fibrocytes in the Human Circulation

Xinyuan Hu, Erin M. DeBiasi, and Erica L. Herzog

 

4 Experimental Approaches to Tissue Injury and Repair in Advanced Age

Aleah L. Brubaker, Stewart R. Carter, and Elizabeth J. Kovacs

 

5 Multicolor Digital Flow Cytometry Used in Human Translational Immunology

Samit Joshi, Subhasis Mohanty, Albert C. Shaw

 

6 Flow Cytometry-Based Methods to Characterize Immune Senescence in Nonhuman Primates

Christine Meyer, Kristen Haberthur, Mark Asquith and Ilhem Messaoudi

 

7 Multiparameter Phenotyping of Human PBMCs Using Mass Cytometry

Michael D. Leipold, Evan W. Newall, and Holden T. Maecker

 

8 Imaging Immunosenescence

Feng Qian and Ruth R. Montgomery

 

9 Activation-induced Cytidine Deaminase and Switched Memory B Cells as Predictors of Effective In Vivo Responses to the Influenza Vaccine

Daniela Frasca, Alain Diaz, and Bonnie B. Blomberg

 

10 Analyzing the Effects of Aging on CD8+ T Cell Phenotype Using Flow Cytometry

Min Sun Shin and Insoo Kang

 

11 Cell-Mediated Immune Response to Influenza

Using Ex Vivo Stimulation and Assays of Cytokine and Granzyme B Responses

Janet C. McElhaney and Beth Gentleman

 

12 Assays for Monitoring Macroautophagy Activity in T Cells

Yair Botbol and Fernando Macian

 

13 Fluorescence-Based Approaches for Quantitative Assessment of Protein Carbonylation, Protein Disulfides and Protein Conformation in Biological Tissues

Ashish R. Chauduri, Rochelle Wei, Arunabh Bhattacharya, and Ryan Hamilton

 

14 Monitoring the DNA Damage Response at Dysfunctional Telomeres

Rekha Rai and Sandy Chang

 

15 Single-Cell Analysis of T Cell Receptor ab Repertoire

Pradyot Dash, George C. Wang, and Paul G. Thomas

 

16 Assessment of B Cell Repertoire in Humans

Yu-Chang Wu, David Kipling, and Deborah Dunn-Walters

 

17 Laboratory and Data Analysis Methods for Characterization of Human B Cell Repertoires by High-throughput DNA Sequencing

Chen Wang, Yi Liu, Krishna M. Roskin, Katherine J.L. Jackson, Scott C. Boyd

 

18 Discovery of Novel MicroRNAs in Aging C. Elegans

Alexandre de Lencastre and Frank Slack

 

19 Analysis of DNA Methylation by Pyrosequencing

Colin Delaney, Sanjay K. Garg, and Raymond Yung