Hepatocyte Transplantation: Methods and Protocols

ISBN-13: 9781493965045 / Angielski / Twarda / 2016 / 365 str.

Peggy Stock; Bruno Christ

Opis

This volume features up-to-date protocols for the isolation, preservation, and validation of various cell sources comprising large and small animal models, examining the impact of cell transplantation on acute and chronic liver diseases. Hepatocyte Transplantation: Methods and Protocols guides readers through laboratory protocols for the generation of humanized livers for the assessment of biological actions in vivo and techniques to monitor cell engraftment after cell transplantation in vivo are described and procedures for computational analyses of hepatocyte transplantation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Hepatocyte Transplantation: Methods and Protocols is an essential resource for researchers and clinicians to assess the biological as well as the therapeutic potential of hepatocyte transplantation.

Spis treści

Hepatocyte Transplantation in Special Populations: Clinical Use in Children.- Alternative Cell Sources to Adult Hepatocytes for Hepatic Cell Therapy.- Late Gestation Fetal Hepatocytes for Liver Repopulation in the Rat.- A Modified Protocol for the Isolation of Primary Human Hepatocytes with Improved Viability and Function from Normal and Diseased Human Liver.- Thy-1 (CD90)-positive Hepatic Progenitor Cells, Hepatoctyes and Non-parenchymal Liver Cells Isolated from Human Livers.- Propagation of Human Hepatocytes in uPA/SCID Mice: Producing Chimeric Mice with Humanized Liver.- Fetal Liver Stem/Progenitor Cell Transplantation: A Model to Study Tissue Mass Replacement and Cell-based Therapies.- Successful Engraftment of Human Hepatocytes in uPA-SCID and FRG® KO Mice.- Gunn rats as Surrogate Model For Evaluation Of Hepatocyte Transplantation-based Therapies of Crigler-Najjar Syndrome Type 1.- Experimental Hepatocyte Transplantation in Pigs.- Demonstrating Potential of Cell Therapy for Wilson´s Disease with the Long‐Evans Cinnamon Rat Model.- Use of Thymidine Kinase Recombinant Adenovirus and Ganciclovir Mediated Mouse Liver Preconditioning for Hepatocyte Xenotransplantation.- Minimally Invasive Liver Preconditioning for Hepatocyte Transplantation in Rats.- Preclinical Swine Models for Monitoring of Hepatocyte Transplantation by MRI.- Deep Digging – Far Red Imaging for the Monitoring of Transplanted Hepatocytes in Rat.- Isolation of GMP grade Human Hepatocytes from Remnant Liver Tissue of Living Donor Liver Transplantation.- Isolation of Hepatocytes and Stellate Cells from a Single Piece of Human Liver.- Microencapsulation of Hepatocytes and Mesenchymal Stem Cells for Therapeutic Applications.- Alginate Encapsulation of Human Hepatocytes and Assessment of Microbeads.- Report on Liver Cell Transplantation Using Human Fetal Liver Cells.- Hepatocyte Transplantation in Children.- Creation of Three-Dimensional Liver Tissue Models from Experimental Images for Systems Medicine.