Experimental Cholestasis Research

ISBN-13: 9781493994229 / Angielski / Miękka / 2020 / 399 str.

Mathieu Vinken

Spis treści

1. Mechanisms of Drug-Induced Cholestasis

            Eva Gijbels and Mathieu Vinken

 

2. LC-MS/MS Analysis of Bile Acids in In Vitro Samples

            Debbie Dewaele, Pieter Annaert, and Eef Hoeben

 

3. Pipe-3D: A Pipeline Based on Immunofluorescence, 3D Confocal Imaging, Reconstructions, and Morphometry for Biliary Network Analysis in Cholestasis

            Amruta Damle-Vartak, Brigitte Begher-Tibbe, Georgia Gunther, Fabian Geisler, Nachiket Vartak, and Jan G. Hengstler

 

4. Vesicle-Based and Hepatocyte-Based Assays for Identification of Drug Candidates Inhibiting BSEP Function

            Pieter Van Brantegem, Neel Deferm, Bing Qi, Tom De Vocht, and Pieter Annaert

 

5. The C-DILI™ Assay:  An Integrated In Vitro Approach to Predict Cholestatic Hepatotoxicity

            Jonathan P. Jackson and Kenneth R. Brouwer

 

6. In Vivo Measurement of Hepatic Drug Transporter Inhibition with Radiolabeled Bile Acids

            Stef De Lombaerde, Sara Neyt, Christian Vanhove, and Filip De Vos

 

7. Measuring the Impact of Bile Acids on the Membrane Order of Primary Hepatocytes and Isolated Mitochondria by Fluorescence Imaging and Spectroscopy

            Tânia Sousa, Rui E. Castro, Ana Coutinho, Cecília M.P. Rodrigues, Manuel Prieto, and Fábio Fernandes

 

8. The Role and Study of Mitochondrial Impairment and Oxidative Stress in Cholestasis

            Reza Heidari and Hossein Niknahad

 

9. Measuring Apoptosis and Necrosis in Cholestatic Liver Injury

            Benjamin L. Woolbright and Hartmut Jaeschke

 

10. Necrosome Formation and Necroptosis in Experimental Cholestasis

            Marta Bento Afonso and Cecília Maria Pereira Rodrigues

 

11. Proteomics in Primary Biliary Cholangitis

            You Li, Ruqui Tang, Patrick S.C. Leung, Eric Gershwin, and Xiong Ma

 

12. Transcriptomics Analysis of Cholestatic Compounds In Vitro

            Céline Parmentier, Philippe Couttet, Marianne Uteng, Armin Wolf, and Lysiane Richert

 

13. Systems Microscopy of Stress Response Pathways in Cholestasis Research

            Johannes P. Schimming, Bas ter Braak, Marije Niemeijer, Steven Wink, and Bob van de Water

 

14. Mouse Models for Diseases in the Cholangiocyte Lineage

            Noémi Van Hul, Urban Lendahl, and Emma R. Andersson

 

15. Common Bile Duct Ligation as Model for Secondary Biliary Cirrhosis

            Sanne Van Campenhout, Hans Van Vlierberghe, and Lindsey Devisscher

 

16. 3,5-Diethoxycarbonyl-1,4-Dihydrocollidine Diet: A Rodent Model in Cholestasis Research

            Elisa Pose, Pau Sancho-Bru, and Mar Coll

 

17. A Rotavirus-Induced Mouse Model to Study Biliary Atresia and Neonatal Cholestasis

            Sujit K. Mohanty, Bryan Donnelly, Haley Temple, and Gregory M. Tiao

 

18. Zebrafish as a Model to Study Cholestatic Liver Diseases

            Duc-Hung Pham and Chunyue Yin

 

19. Set-Up and Use of HepaRG Cells in Cholestasis Research

            Christiane Guguen-Guillouzo and André Guillouzo

 

20. Organotypic 3D HepaRG Liver Model for Assessment of Drug-Induced Cholestasis

            Sreenivasa C. Ramaiahgari and Stephen S. Ferguson

 

21. Establishment of Sandwich Cultures of Primary Human Hepatocytes

            Eva Gijbels, Tamara Vanhaecke, and Mathieu Vinken

 

22. Detection of Drug-Induced Cholestasis Potential in Sandwich-Cultured Human Hepatocytes

            Neel Deferm, Lysiane Richert, Pieter Van Brantegem, Tom De Vocht, Bing Qi, Peter de Witte, Thomas Bouillon, and Pieter Annaert

 

23. Ex Vivo Model in Cholestasis Research

            Ruby E.H. Karsten, Dorenda Oosterhuis, Louise A. van Wijk, and Peter Olinga

 

24. Patient-Derived Organoids from Human Bile: An In Vitro Method to Study Cholangiopathies

            Carol J. Soroka, David N. Assis, and James L. Boyer

 

25. Use of Biliary Organoids in Cholestasis Research

            Fotios Sampaziotis, Olivia Tysoe, Teresa Brevini, and Ludovic Vallier

 

26. In Silico Approaches to Predict Drug-Transporter Interaction Profiles: Data Mining, Model Generation, and Link to Cholestasis

            Sankalp Jain and Gerhard F. Ecker

Blurb

This detailed book provides a state-of-the-art compilation of protocols relevant for the experimental study of cholestasis, each written by internationally recognized research teams that have built up long-standing expertise regarding one or more aspects of experimental cholestasis research. The first half of the collection examines methods targeted towards studying established and new mechanisms of cholestasis, while the second half explores experimental models and tools to investigate mechanisms of cholestasis and/or to predict cholestatic properties of drugs. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Experimental Cholestasis Research serves as an ideal guide for basic and applied researchers pursuing this vital area of research in pharmacology and toxicology.