Epithelial Cell Culture Protocols

ISBN-13: 9781617372414 / Angielski / Miękka / 2010 / 406 str.

Clare Wise

Opis

There have been significant advances in research involving the isolation and culture of epithelial cells in the past decade, and many new techniques have been developed. Monolayer cultures can be used to evaluate the nature and behavior of cells, while the use of epithelial cells in model systems has allowed a deeper understanding of cellular and molecular mechanisms and interactions. The aim of this book is to provide a comprehensive, step-by-step guide to many techniques for epithelial cell culture, combining in one volume the more commonly used protocols along with many that are more speci- ized. Epithelial Cell Culture Protocols should help those who are new to this field and want to learn the basic culture techniques, as well as those needing to use more wide ranging and specific protocols. It should be a useful resource on its own, and also complement the other volumes that have been written about cell culture in the Methods in Molecular Biology series. Epithelial Cell Culture Protocols covers a wide variety of protocols, mostly aimed at the researcher, but also a few aimed at clinicians. The est- lishment and maintenance of primary cultures derived from many different tissues and different species is covered. Particular emphasis has been placed on protocols needed to further analyze and assess epithelial cells, for example, by looking at apoptosis and integrins and by measuring membrane capa- tance and confluence. Using different co-culture techniques, it is possible also to develop models to investigate many different systems in vitro.

Recenzje

"The book could be used effectively by experienced or novice basic science researchers or clinicians since the protocols are written clearly and concisely. There are 31 brief chapters in this book and about 60 authors. They are experienced and knowledgeable researchers in the field. . .The protocols are written clearly and should serve as a useful laboratory resource for anyone wishing to learn how to culture these cells. . .would be quite useful for anyone wishing to place epithelial cells in culture."-Doody's Health Sciences Book Review Journal

Spis treści

Human Lens Epithelial Cell Culture Nobuhiro Ibaraki Human Airway Epithelial Cell Culture Mutsuo Yamaya, Masayoshi Hosoda, Tomoko Suzuki, Norihiro Yamada, and Hidetada Sasaki Rat Gastric Mucosal Epithelial Cell Culture Yoshitaka Konda and Tsutomu Chiba Thymic Epithelial Cell Culture Carsten Röpke Bile Duct Epithelial Cell Culture Alphonse E. Sirica Liver Epithelial Cell Culture Rolf Gebhardt Pulmonary Epithelial Cell Culture Ben Forbes Prostate Epithelial Cell Isolation and Culture David L. Hudson A Bovine Mammary Endothelial/Epithelial Cell Culture Model of the Blood/Milk Barrier Albert Guidry and Celia O'Brien The Blood-CSF Barrier in Culture: Development of a Primary Culture and Transepithelial Transport Model for Choroidal Epithelial Cells Wei Zheng and Qiuqu Zhao An In Vitro Model of Differentiated Human Airway Epithelia: Methods for Establishing Primary Cultures Philip H. Karp, Thomas O. Moninger, S. Pary Weber, Tamara S. Nesselhauf, Janice L. Launspach, Joseph Zabner, and Michael J. Welsh Primary Mouse Keratinocyte Culture Kairbaan Hodivala-Dilke Analyzing Apoptosis in Cultured Epithelial Cells Andrew P. Gilmore and Charles H. Streuli Keratins as Markers of Epithelial Cells David L. Hudson Epithelial Cell Integrins Joachim Rychly Isolation, Cultivation, and Differentiation of Normal Human Epidermal Keratinocytes in Serum-Free Medium Sebastian Zellmer and Dieter Reissig Clinical Application of Autologous Cultured Epithelia for the Treatment of Burns and Disfigurement of Skin Surfaces Norio Kumagai Transplantation of Cultured Epithelial Cells Minoru Ueda, Yukio Sumi, Yoshitaka Hibino, and Ken-Ichiro Hata An Outgrowth Culture System of Normal Human Epithelium: An In Vitro Modelto Study the Coordination of Cellular Migration and Proliferation Tohru Masui Application of Epithelial Cell Culture in Drug Transport in the Respiratory Tract Jie Shen and Vincent H. L. Lee Applications of Epithelial Cell Culture in Studies of Drug Transport Staffan Tavelin, Johan Gråsjö, Jan Taipalensuu, Göran Ocklind, and Per Artursson X-Ray Microanalysis of Epithelial Cells in Culture Godfried M. Roomans ENU Mutagenesis of Rat Mammary Epithelial Cells George Stoica ENU-Induced Ovarian Cancer George Stoica Measurement of Membrane Capacitance in Epithelial Monolayers Carol A. Bertrand and Ulrich Hopfer Assessing Epithelial Cell Confluence by Spectroscopy Simon A. Lewis Co-Cultivation of Liver Epithelial Cells with Hepatocytes Rolf Gebhardt Co-Culture and Crosstalk between Endothelial Cells and Vascular Smooth Muscle Cells Mediated by Intracellular Calcium Wee Soo Shin, Chieko Hemmi, and Teruhiko Toyo-oka Establishing Epithelial-Immune Cell Co-Cultures: Effects on Epithelial Ion Transport and Permeability Derek M. McKay and Mary H. Perdue Explant Cultures of Embryonic Epithelium: Analysis of Mesenchymal Signals Carin Sahlberg, Tuija Mustonen, and Irma Thesleff Bacterial Interactions with Host Epithelium In Vitro Nicola Jones, Mary H. Perdue, Philip M. Sherman, and Derek M. McKay

Blurb

Many powerful new techniques for the isolation and culture of epithelial cells have been developed in the past decade. In Epithelial Cell Culture Protocols, a team of well-versed experimenters and clinical researchers share their best methods for establishing and maintaining epithelial cell cultures, for analyzing and studying their characteristics, and for using them to set up models of critical biological systems. These readily reproducible techniques describe all the commonly used protocols, as well as those that are more specialized, including several aimed at clinical investigation. The emphasis is on the analysis and assessment of epithelial cells, for example, by looking at apoptosis and integrins or by measuring membrane capacitance and confluence. Also described in step-by-step detail are co-culture techniques valuable in developing models for investigating many different in vitro systems, including the blood-brain barrier, drug uptake, and the interaction of epithelial cells with bacteria. Several protocols cover the culturing of epithelial cells and their use in treating patients with burns and other skin disorders.
Wide-ranging and highly practical, Epithelial Cell Culture Protocols offers both novices and expert investigators alike a step-by-step guide toward a deeper understanding of cellular and molecular mechanisms in general, as well as a set of robust techniques for specifically evaluating the nature and behavior of epithelial cells.