Epiblast Stem Cells: Methods and Protocols

ISBN-13: 9781627036276 / Angielski / Twarda / 2013 / 216 str.

Ramiro Alberio

Opis

In Epiblast Stem Cells: Methods and Protocols, expect researchers in the field provide a detailed collection of techniques and protocols useful to the study of the biology of the pluripotent epiblast. These include methods and techniques used to study epiblast development in different amniotes. This collection brings together contributions from the fields of embryology, stem cell biology and developmental biology together, providing a single volume with detailed procedures for the isolation and culture of epiblasts at different stages of development, and techniques for the study of differentiation into specific lineages. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, a complete list of the necessary materials and reagents, detailed laboratory protocols, and extensive notes providing suggestions on troubleshooting and how to overcome common difficulties. Comprehensive and cutting-edge, Epiblast Stem Cells: Methods and Protocols serves as a resource to individuals interested in studying the biology of pluripotent cells.

Spis treści

1. Generation and Characterization of Epiblast Stem Cells from Blastocyst-stage Mouse Embryos

Daniel C. Factor, Fadi J. Najm, and Paul J. Tesar

 

2. Reversion of Mouse Post-Implantation Epiblast Stem Cells to a Naïve Pluripotent State by Modulation of Signalling Pathways

Astrid Gillich, Siqin Bao, and M. Azim Surani

 

3. Converting Mouse Epiblast Stem Cells into Mouse Embryonic Stem Cells by using Small Molecules

Wenlin Li and  Sheng Ding

 

4. Isolation and Culture of Rabbit Embryonic Stem Cells

Arata Honda

 

5. Goat Embryonic Stem-like Cell Derivation and Characterization

Esmail Behboodi, Louise Lam, William G. Gavin, Alla Bondareva, Ina Dobrinski

 

6. Derivation and Culture of Canine Embryonic Stem Cells

Ian C. Tobias, Courtney R. Brooks, Jonathan H. Teichroeb, and  Dean H. Betts

 

7. Isolation and Culture of Porcine Embryonic Stem Cells
Ivan Vassiliev and Mark B. Nottle

 

8. Isolation and Culture of Pig Epiblast Stem Cells

Aida Rodriguez, David A. Contreras and Ramiro Alberio

 

9. Isolation and Culture of Bovine Embryonic Stem Cells

Shanbo Cao, Fang Wang, and Lin Liu

 

10. Analysis of Molecular Markers for Staging Peri-gastrulating Bovine Embryos

Isabelle Hue, Severine A Degrelle,  and Christoph Viebahn      

 

11. Chicken Embryonic Stem Cells: Establishment and Characterization

Pauline Aubel  and Bertrand Pain

 

12. Manipulating the Avian Epiblast and Epiblast-derived Stem Cells

Cantas Alev, Mikiharu Nakano, Yuping Wu, Hiroyuki Horiuchi, and Guojun Sheng

 

13. Stepwise Differentiation from Naïve State Pluripotent Stem Cells to Functional Primordial Germ Cells through an Epiblast-like State

Katsuhiko Hayashi and Mitinori Saitou

 

14. Isolation and Culture of Porcine Neural Progenitor Cells from Embryos and Pluripotent Stem Cells

MA Rasmussen, VJ Hall,  and P Hyttel

 

15. Derivation of Neural Precursors from Bovine Pre-implantation Embryos

Giovanna Lazzari, Silvia Colleoni, and Cesare Galli