Drosophila Cells in Culture

ISBN-13: 9780128094730 / Angielski / Miękka / 2017 / 258 str.

Guy Echalier; Norbert Perrimon

Opis

Drosophila Cells in Culture, Second Edition, includes comprehensive coverage of cell lines, methods for creating cell lines, methods for genome engineering, and the use of cell lines for genome wide rNAi screens. This publication summarizes over thirty years of experience in the handling of in vitro cultured Drosophila cells alongside recent methods and functional screens. Early and experienced researchers studying drosophila in developmental biology, genetics, neuroscience, and across the biological and biomedical sciences will benefit from this expert knowledge.

• Offers full coverage of cell lines and primary cultures
• Provides a go-to resource for methods and studies completed with drosophila cells in culture
• Presents a wide spectrum of experimental techniques

Spis treści

1. Composition of the Body Fluid of Drosophila and the Design of Culture Media for Drosophila Cells2. Primary Cell Cultures of Drosophila Embryonic Cells3. Continuous Drosophila Cell Lines Established In Vitro4. Cells or Tissues In Course of Differentiation5. Hormonal Control of The Development of Insects: Implications In Organ or Cell Cultures of Drosophila6. Gene Transfer into Cultured and Applications7. Rnai and Crispr (Cas9, Cas9-Act, Cas9-Repressor) Methods to Perturb Gene Function8. Functional Genomics Screens Using Rnai, Crispr, and Orf Overexpression

O autorze

Guy Echalier is an esteemed expert in the research of Drosophilia, having over thirty years experience handling in vitro cultured Drosophilia cells. Norbert Perrimon is a leading researcher in developmental biology. His current research pertains to using Drosophilia as a model system to characterize the responses of cells to external signalling. Stephanie E. Mohr is a leader in the field of high-throughput functional genomics screening in Drosophila cultured cells.