Chromosome and Genomic Engineering in Plants: Methods and Protocols

ISBN-13: 9781493981632 / Angielski / Miękka / 2018 / 218 str.

Minoru Murata

Spis treści

1. Production of Engineered Minichromosome Vectors via the Introduction of Telomere Sequences
Nathaniel Graham, Nathan Swyers, Jon Cody, Morgan McCaw, Changzeng Zhao, and James A. Birchler

2. Method for Biolistic Site-Specific Integration in Rice and Tobacco Catalyzed by Bxb1 Integrase

Ruyu Li, Zhiguo Han, Lili Hou, Gurminder Kaur, Qian Yin, and David W. Ow

3. Protocol for In Vitro Stacked Molecules Compatible with In Vivo Recombinase Mediated Gene Stacking

Weiqiang Chen and David W. Ow

4. Generation and Analysis of Transposon Ac/Ds-Induced Chromosomal Rearrangements in Rice Plants

Yuan Hu Xuan, Thomas Peterson, and Chang-deok Han

5. One-Step Generation of Chromosomal Rearrangements in Rice

Minoru Murata, Asaka Kanatani, and Kazunari Kashihara

6. Genome Elimination by Tailswap CenH3: In Vivo Haploid Production in Arabidopsis thaliana

Ravi Maruthachalam and Ramesh Bondada

7. Gametocidal Systems for Dissecting Wheat Chromosomes

Blurb

This volume assembles protocols for chromosome engineering and genome editing in two recently developed approaches for manipulating chromosomal and genomic DNA in plants. The first approach is a “plant chromosome vector” system, which allows the introduction of desired genes or DNA into target sites on the chromosome vector, particularly by sequence-specific recombination. The second approach is “genome-editing,” which makes it possible to introduce mutations into any of the genes of DNA that we wish to change. In addition, this book also covers other related techniques used to accelerate progress in plant chromosome and genome engineering. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Chromosome and Genomic Engineering in Plants: Methods and Protocols provides a comprehensive source of protocols and other necessary information to anyone interested in this field of study.