Zebrafish

ISBN-13: 9781071634004 / Angielski / Twarda / 2023

Opis

This fully updated edition introduces new tools, models, and analytic insights that position the zebrafish even more strongly as an engine of discovery for developmental and disease biology. Beginning with a section exploring detailed methods for use of zebrafish to model a variety of human diseases, the book continues by illuminating the key ongoing role of the fish model in studies of the vertebrate nervous system, tools and approaches using zebrafish to study stem cell and regenerative biology, as well as techniques in genetics and genomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Zebrafish: Methods and Protocols, Third Edition serves as an invaluable guide to propel advances in developmental biology, disease modeling, and regeneration research using zebrafish and medaka as model systems.

Spis treści

Part I: Disease Models

 

1. Delivering Traumatic Brain Injury to Larval Zebrafish

            Taylor Gill, Laszlo Locskai, Alexander H. Burton, Hadeel Alyenbaawi, Travis Wheeler, Edward A. Burton, and W. Ted Allison

 

2. Functional Genomics of Novel Rhabdomyosarcoma Fusion-Oncogenes Using Zebrafish

            Matthew Kent, Katherine Silvius, Jack Kucinski, Delia Calderon, and Genevieve C. Kendall

 

3. Methods to Study Liver Disease Using Zebrafish Larvae

            Elena Magnani, Anjana Ramdas Nair, Ian McBain, Patrice Delaney, Jaime Chu, and Kirsten C. Sadler

 

4. Developmental Toxicity Assessment Using Zebrafish-Based High Throughput Screening

            Subham Dasgupta, Michael T. Simonich, and Robyn L. Tanguay

 

5. Cancer Modeling by Transgene Electroporation in Adult Zebrafish (TEAZ)

            Emily Montal, Shruthy Suresh, Yilun Ma, Mohita M. Tagore, and Richard M. White

 

6. Lineage Tracing of Bone Cells in the Regenerating Fin and during Repair of Bone Lesions

            Tan Wen Hui and Christoph Winkler

 

Part II: Neuro

 

7. Primary Culture of Neuronal Populations for Various Downstream Applications

            Richard Taylor and Corinne Houart

 

8. Holographic Optogenetic Activation of Neurons Eliciting Locomotion in Head-Embedded Larval Zebrafish

            Xinyu Jia and Claire Wyart

 

9. Brain Imaging and Registration in Larval Zebrafish

            Ashwin A. Bhandiwad, Tripti Gupta, Abhignya Subedi, Victoria Heigh, George A. Holmes, and Harold A. Burgess

 

10. Simultaneous Behavioral and Neuronal Imaging by Tracking Microscopy

            Drew N. Robson and Jennifer M. Li

 

11. Genetic Identification of Neural Circuits Essential for Active Avoidance Fear Conditioning in Adult Zebrafish

            Pradeep Lal, Hideyuki Tanabe, and Koichi Kawakami

 

Part III: Regeneration

 

12. Quantitative Live Imaging of Zebrafish Scale Regeneration: From Adult Fish to Signaling Patterns and Tissue Flows

            Alessandro De Simone

 

13. Generation of Conditional Knockout Zebrafish Using an Invertible Gene-Trap Cassette

            Masahito Ogawa and Kazu Kikuchi

 

14. Spinal Cord Injury and Assays for Regeneration

            Brooke Burris and Mayssa H. Mokalled

 

15. Selective Cell Ablation Using an Improved Prodrug-Converting Nitroreductase

            Timothy S. Mulligan and Jeff S. Mumm

 

16. Section Immunostaining for Protein Expression and Cell Proliferation Studies of Regenerating Fins

            Scott Stewart and Kryn Stankunas

 

Part IV: Genetics and Genomics

 

17. In Vivo Optogenetic Phase Transition of an Intrinsically Disordered Protein

            Kazuhide Asakawa, Hiroshi Handa, and Koichi Kawakami

 

18. Colorimetric Barcoding to Track, Isolate, and Analyze Hematopoietic Stem Cell Clones

            Dorothee Bornhorst, Brandon Gheller, and Leonard I. Zon

 

19. Mutation Knock-In Methods Using Single-Stranded DNA and Gene Editing Tools in Zebrafish

            Sergey V. Prykhozhij and Jason N. Berman

 

20. Generation of Transgenic Fish Harboring CRISPR/Cas9-Mediated Somatic Mutations via a tRNA-Based Multiplex sgRNA Expression

            Tomoya Shiraki and Koichi Kawakami

 

21. Scalable CRISPR Screens in Zebrafish Using MIC-Drop

            Saba Parvez, Tejia Zhang, and Randall T. Peterson

 

22. The Goldfish Genome and Its Utility for Understanding Gene Regulation and Vertebrate Body Morphology

            Yoshihiro Omori and Shawn M. Burgess