Z-DNA: Methods and Protocols

ISBN-13: 9781071630839 / Angielski

Kyeong Kyu Kim; Vinod Kumar Subramani

Opis

This detailed volume compiles key methods and techniques used to establish some of the structural and functional aspects of Z-form nucleic acids. Beginning with chapters on characterizing Z-DNA, the book continues by exploring structure determination techniques, gene- and genome-targeting methods and genome-wide approaches, Z-RNA study, and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Z-DNA: Methods and Protocols serves as an ideal guide for students and researchers curious about this unique Z nucleic acid as an area of investigation, aiming to help the field grow, flourish, and unravel the hidden and novel roles of Z. 

Chapter 1 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

This is an open access book.

Spis treści

1. The Origin of Left-Handed Poly[d(G-C)]

            Thomas M. Jovin

 

2. Characterization of Z-DNA Using Circular Dichroism

            Vinod Kumar Subramani and Kyeong Kyu Kim

 

3. Characterization of Z-DNA by Infrared Spectroscopy

            Fengqiu Zhang and Qing Huang

 

4. Crystallization of Z-DNA in Complex with Chemical and Z-DNA Binding Z-Alpha Protein

            MinSoung Kang and Doyoun Kim

 

5. NMR Titration Studies in Z-DNA Dynamics

            Seo-Ree Choi, Kwang-Im Oh, Yeo-Jin Seo, and Joon-Hwa Lee

 

6. Single-Molecule Methods to Study Z-DNA Mechanics and Dynamics

            Hae Jun Jung, Beom-Hyeon Park, Sook Ho Kim, and Seok-Cheol Hong

 

7. BZ Junctions and Its Application as Probe (2AP) to Detect Z-DNA Formation and Its Effector

            MinSoung Kang and Doyoun Kim

 

8. Oligonucleotide Containing 8-Trifluoromethyl-2’-Deoxy-Guanosine as a Z-DNA Probe

            Hong-Liang Bao and Yan Xu

 

9. Chiroptical Properties of Z-DNA Using Ionic Porphyrins and Metalloporphyrins

            Alessandro D’Urso

 

10. Construction of a Z-DNA Specific Recombinant Nuclease Zαα-FOK for Conformation Studies

            Seul Ki Lee and Yang-Gyun Kim

 

11. Human Heme Oxygenase-1 Promoter Activity is Mediated by Z-DNA Formation

            Atsushi Inose-Maruyama, Shuya Kasai, and Ken Itoh

 

12. ChIP-Seq Strategy to Identify Z-DNA Forming Sequences in the Human Genome

            Tae-Young Roh

 

13. Detection of Z-DNA Structures in Supercoiled Genome

            Fedor Kouzine, Damian Wojtowicz, Teresa M. Przytycka, and David Levens

 

14. Thermogenomic Analysis of Left-Handed Z-DNA Propensities in Genomes

            Ryan S. Czarny and P. Shing Ho

 

15. DeepZ: A Deep-Learning Approach for Z-DNA Prediction

            Nazar Beknazarov and Maria Poptsova

 

16. Methods to Study Z-DNA-Induced Genetic Instability

            Guliang Wang, Laura Christensen, and Karen M. Vasquez

 

17. Single-Molecule Visualization of B-Z Transition in DNA Origami Using High-Speed AFM

            Masayuki Endo and Hiroshi Sugiyama

 

18. Adoption of A-Z Junctions in RNAs by Binding of Zα Domains

            Parker J. Nichols, Shaun Bevers, Morkos Henen, Jeffrey S. Kieft, Quentin Vicens, and Beat Vögeli

 

19. Detecting Z-RNA in Mammalian Cells

            Chaoran Yin, Ting Zhang, and Siddharth Balachandran

 

20. Identification of ADAR1 p150 and p110 Associated Edit Sites

            Tony Sun, Brad R. Rosenberg, Hachung Chung, and Charles M. Rice

 

21. Z-DNA and Z-RNA: Methods Past and Future

            Alan Herbert

Blurb

This detailed volume compiles key methods and techniques used to establish some of the structural and functional aspects of Z-form nucleic acids. Beginning with chapters on characterizing Z-DNA, the book continues by exploring structure determination techniques, gene- and genome-targeting methods and genome-wide approaches, Z-RNA study, and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Z-DNA: Methods and Protocols serves as an ideal guide for students and researchers curious about this unique Z nucleic acid as an area of investigation, aiming to help the field grow, flourish, and unravel the hidden and novel roles of Z. 

Chapter 1 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.