Yeast Surface Display: Methods, Protocols, and Applications

ISBN-13: 9781493946679 / Angielski / Miękka / 2016 / 271 str.

Bin Liu

Opis

In addition to research and discovery, yeast surface display technology has found applications in industrial processes such as biofuel production and environmental pollutant absorption and degradation. Yeast Surface Display: Methods, Protocols, and Applications guides readers through yeast surface antibody display library and antibody engineering, yeast surface display as a tool for protein engineering, yeast surface cDNA display library construction and applications, and yeast surface display in bioassay and industrial applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Concise and easy-to-use, Yeast Surface Display: Methods, Protocols, and Applications aims to help accelerate the work of protein chemists, antibody engineers, molecular and cell biologists, and industrial bioengineers. ​

Spis treści

Part I: Yeast Surface Antibody Display Library and Antibody Engineering

 

1: Protein Engineering and Selection Using Yeast Surface Display

Alessandro Angelini, Tiffany F. Chen, Seymour de Picciotto, Nicole J. Yang, Alice Tzeng, Michael S. Santos, James A. Van Deventer, Michael W. Traxlmayr, and K. Dane Wittrup

 

2: Isolation and Validation of Anti-B7-H4 scFvs from an Ovarian Cancer scFv yeast-Display Library

Denarda Dangaj and Nathalie Scholler

 

3: Combining Phage and Yeast Cell Surface Antibody Display to Identify Novel Cell Type-Selective Internalizing Human Monoclonal Antibodies

Scott Bidlingmaier, Yang Su and Bin Liu

 

4: Yeast Display-Based Antibody Affinity Maturation Using Detergent-Solubilized Cell Lysates

Benjamin J. Tillotson, Jason M. Lajoie and Eric V. Shusta

 

Part II: Yeast Surface Display as a Tool For Protein Engineering

5: Yeast Endoplasmic Reticulum Sequestration Screening for the Engineering of Proteases from Libraries Expressed in Yeast

Li Yi, Joseph M. Taft, Qing Li, Mark C. Gebhard, George Georgiou, Brent L. Iverson

 

6: T Cell Receptor Engineering and Analysis Using the Yeast Display Platform

Sheena N. Smith, Daniel T. Harris, and David M. Kranz

 

7: Epitope Specific Binder Design by Yeast Surface Display

Jasdeep K. Mann and Sheldon Park

 

8: Applications of Yeast Surface Display for Protein Engineering

Gerald M. Cherf and Jennifer R. Cochran

 

PART III : Yeast Surface cDNA Display Library Construction and Applications

 

9 : Identification of Novel Protein-Ligand Interactions by Exon Microarray Analysis of Yeast Surface Displayed cDNA Library Selection Outputs

Scott Bidlingmaier and Bin Liu

 

10: Identification of Post-Translational Modification-Dependent Protein Interactions using Yeast Surface Displayed Human Proteome Libraries

Scott Bidlingmaier and Bin Liu

 

11: Utilizing Yeast Surface Human Proteome Display Libraries to Identify Small Molecule-Protein Interactions

Scott Bidlingmaier and Bin Liu

 

Part IV: Yeast Surface Display in Bioassay and Industrial Applications

 

12: Enzyme Evolution by Yeast Cell Surface Engineering

Natsuko Miura, Kouichi Kuroda, and Mitsuyoshi Ueda

 

13: Electrochemical Glucose Biosensor Based on Glucose Oxidase Displayed on Yeast Surface

Hongwei Wang, Qiaolin Lang, Bo Liang, and Aihua Liu

 

14: Couple Binding to Catalysis – Use Yeast Cell Surface Display to Select Enzymatic Activities

Keya Zhang, Karan Bhuripanyo, Yiyang Wang, and Jun Yin

 

15: The use of Yeast Surface Display in Biofuel Cells

Alon Szczupak and Lital Alfonta