Yeast Metabolic Engineering: Methods and Protocols

ISBN-13: 9781071623985 / Angielski / Twarda / 2022

Mapelli, Valeria

Opis

This second edition volume expands on the previous edition with new and updated chapters on the latest developments in the study of yeast within the biotechnology field. The chapters in this book cover topics such as transformation protocols for genetic engineering of Saccaromyces cerevisiae and Komagataella spp.; an overview of selection markers, promoters, and strains used for metabolic engineering of S. cerevisiae, P. pastoris, and Z. bailii; the use of yeast in CRISPR/Cas9 technology; tools to study metabolic pathway in Yarrowia lypolitica; and a discussion on the “universal expression system” that is applied in a broad spectrum of fungal species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Yeast Metabolic Engineering: Methods and Protocols, Second Edition is a valuable resource for researchers and scientists interested in learning more about this important and developing field.

Spis treści

Preface…
Table of Contents…
Contributing Authors…

1. An Overview on Selectin Marker Genes for Transformation of Saccharomyces cerevisiae
Verena Siewers

2. High Efficiency Plasmid DNA Transformation in Yeast
O’Taveon R. Fitzgerald, Nestor D. Rodriguez, and L. Kevin Lewis

3. CasPER, A CRISPR/Cas9-Based Method for Directed Evolution in Genomic loci in Saccharomyces cerevisiae
Tadas Jakočiūnas, Michael K. Jensen, and Jay D. Keasling

4. Fluorescence Activated Cell Sorting as a Tool for Recombinant Strain Screening
Christos Skrekas, Raphael Ferreira, and Florian David

5. Generation of Arming Yeasts with Active Proteins and Peptides via Cell Surface Display System: Cell Surface Engineering, Bio-Arming Technology
Kouichi Kuroda and Mitsuyoshi Ueda

6. Strains and Molecular Tools for Recombinant Protein Production in Pichia pastoris
Claudia Rinnofner, Michael Felber, and Harald Pichler

7. Competent Cell Preparation and Transformation of Pichia pastoris
Joan Lin-Cereghino, Christopher A. Naranjo, and Geoff P. Lin-Cereghino

8. CRISPR/Cas9 Tool Kit for Efficient and Targeted Insertion/Deletion Mutagenesis of the Komagataella phaffii (Pichia pastoris) Genome
Jasmin Elgin Fischer and Anton Glieder

9. Plasmid-Based Gene Knockout Strategy with Subsequent Marker Recycling in Pichia pastoris
Simon Kobalter, Astrid Radkohl, Helmut Schwab, Anita Emmerstorfer-Augustin, and Harald Pichler

10. Engineering of Promoters for Gene Expression in Pichia pastoris
Thomas Vogl

11. Molecular Tools for Leveraging the Potential of the Acid Tolerant Yeast Zygosaccharomyces bailii as Cell Factory
Paola Branduardi, Liliane Barroso, Laura Dato, Edward J. Louis, and Danilo Porro

12. Golden Gate Multi-Genes Assembly Method for Yarrowia lipolytica
Macarena Larroude, Jean-Marc Nicaud, and Tristan Rossignol

13. A Universal Gene Expression System for Novel Yeast Species
Dominik Majzita, Anssi Rantasalo, and Marja Ilmén

14. Dynamic Feeding of Pichia pastoris
Alexander Pekarsky and Oliver Spadiut

15. Automated Evolutionary Engineering of Yeasts
Erik de Hulster, Christiaan Mooiman, Rowin Timmermans, and Robert Mans

16. Reconstruction of Genome-Scale Metabolic Model of Hansenula polymorpha using RAVEN
Francisco Zorrilla and Eduard J. Kerkhoven

Subject Index List...