Yeast Gene Analysis: Volume 36

ISBN-13: 9780123694782 / Angielski / Twarda / 2007 / 700 str.

Ian Stansfield; Michael JR Stark

Opis

Focusing on Saccharomyces cerevisiae, the second edition of Yeast Gene Analysis represents a major reworking of the original edition, with many completely new chapters and major revisions to all previous chapters. Originally published shortly after completion of the yeast genome sequence, the new edition covers many of the major genome-wide strategies that have been developed since then such as microarray analysis of transcription, synthetic gene array studies, protein microarrays and chemical genetic approaches. It represents a valuable resource for any research laboratory using budding yeast as their experimental system in which to identify new yeast gene functions. The chapters are written in a readable style with useful background information, technical tips and specific experimental protocols included as appropriate, enabling both the novice and the experienced yeast researcher to adopt new procedures with confidence.

• New chapters on: Strain construction; genome-wide two-hybrid approaches; use of microarrays for transcript analysis; real-time analysis of chromosome behaviour and FRET; synthetic gene array technology and protein arrays; chemical genomics and yeast prions; RNA gene analysis and mitochondrial gene function analysis; phylogenetic footprinting; discovering human gene function and predicting yeast gene function

Spis treści

Introduction to functional analysis in yeast
Yeast genetics and strain construction
Yeast transformation
A guided tour to PCR-based genomic manipulations of S. cerevisiae (PCR-targeting)
Studying essential genes:
generating and using promoter fusions and conditional alleles
Yeast hybrid approaches
Array-based yeast two-hybrid screening for protein-protein interactions
Reporter genes and their uses in studying yeast gene expression
Transcript analysis: a microarray approach
GFP-based microscopic approaches for whole chromosome analysis in yeasts
Immunological methods
Measuring the proximity of proteins in living cells by fluorescence resonance energy transfer between CFP and YFP
Identification, characterization, and phenotypic analysis of covalently linked cell wall Proteins
Yeast protein microarrays
Smart genetic screens
High-throughput strain construction and systematic synthetic lethal Screening in Saccharomyces cerevisiae
Chemical genomic tools for understanding gene function and drug action
RNA gene analysis
Analysis of gene function of mitochondria
Yeast prions and their analysis in vivo
Metabolic control in the eukaryotic cell, a Systems Biology perspective
Phylogenetic footprinting
Saccharomyces cerevisiae as a tool for human gene function discovery
Bioinformatic prediction of yeast gene function
Yeast genetic strain and plasmid collections
Yeast gene analysis: the future