Yeast Cytokinesis: Methods and Protocols

ISBN-13: 9781493979899 / Angielski / Miękka / 2019 / 395 str.

Alberto Sanchez-Diaz;Pilar Perez

Opis

This volume presents protocols on yeast cytokinesis that will help Molecular and Cellular Biology researchers in the use of these microorganisms to approach the study of general or specific key questions in cytokinesis. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Yeast Cytokinesis: Methods and Protocols provides practical and step-by-step detailed protocols useful for a wide audience ranging from experienced researchers to beginners in the use of yeasts.

Spis treści

1. Time-lapse Fluorescence Microscopy Of Budding Yeast Cells

Arun Kumar and Manuel Mendoza

 

2. Real-time Visualization and Quantification of Contractile Ring Proteins in Single Living Cells

Reshma Davidson, Yajun Liu, Kenneth S. Gerien, and Jian-Qiu Wu

 

3. Fluorescence Recovery After Photo-bleaching (FRAP) and Fluorescence Loss in Photo-bleaching (FLIP) Experiments to Study Protein Dynamics during Budding Yeast Cell Division

Alessio Bolognesi, Andrzjei Sliwa-Gonzalez, Rupali Prasad, and Yves Barral

 

4. High-Speed Super Resolution Imaging of Live Fission Yeast Cells

Caroline Laplante, Fang Huang, Joerg Bewersdorf,  and Thomas D. Pollard

 

5. Monitoring Chitin Deposition during Septum Assembly in Budding Yeast

Arcones, Irene and Roncero Cesar

 

6. Imaging Septum Formation by Fluorescence Microscopy

J.C. Ribas and J.C.G. Cortés

 

7. Visualization of Cytokinesis Events in Budding Yeast by Transmission Electron Microscopy

Franz Meitinger and Gislene Pereira

 

8. Visualization of Fission Yeast Cells By Transmission Electron Microscopy

Matthias Sipiczki

 

9. Characterization of Septin Ultrastructure in Budding Yeast Using Electron Tomography

Aurélie Bertin and Eva Nogales

 

10. Isolation of Cytokinetic Actomyosin Rings from Saccharomyces cerevisiae and Schizosaccharomyces pombe

Junqi Huang,  Mithilesh Mishra, Saravanan Palani, Ting Gang Chew, Mohan K. Balasubramanian

 

11. Measurements of Myosin-II motor Activity during Cytokinesis In Fission Yeast

Qing Tang, Luther W. Pollard, and  Matthew Lord

 

12. In Vitro Biochemical Characterization of Cytokinesis Actin Binding Proteins

Dennis Zimmermann, Alisha N. Morganthaler, David R. Kovar,  and Cristian Suarez

 

13. Characterization of Cytokinetic F-BARs and other Membrane Binding Proteins

Nathan A. McDonald and Kathleen L. Gould

 

14. Analysis of Three-Dimensional Structures of Exocyst Components

Johannes Lesigang and Gang Dong

 

15. Analysis of Rho-GTPase Activity during Budding-yeast Cytokinesis

Masayuki Onishi and John R. Pringle

 

16. Detection of Phosphorylation Status of Cytokinetic Components

Franz Meitinger, Saravanan Palani, and Gislene Pereira

 

17. Studying Protein-Protein Interactions in Budding Yeast Using Co-Immunoprecipitation

Magdalena Foltman and Alberto Sanchez-Diaz

 

18. Conditional Budding Yeast Mutants with Temperature-Sensitive and Auxin-Inducible Degrons for Screening Of Suppressor Genes

Asli Devrekanli and Masato T. Kanemaki

 

19. Synchronization of the Budding Yeast Saccharomyces cerevisiae

Magdalena Foltman, Iago Molist, and Alberto Sanchez-Diaz

 

20. Fission Yeast Cell Cycle Synchronization Methods

Marta Tormos-Pérez, Livia Pérez-Hidalgo, and Sergio Moreno

 

21. A Review of Fluorescent Proteins for Use in Yeast

Maja Bialecka-Fornal, Tatyana Makushok, and Susanne M. Rafelski

 

22. Visualisation and Image Analysis of Yeast Cells

Steve Bagley

 

23. Toolbox for Protein Structure Prediction

Daniel Barry Roche and Liam James McGuffin

 

24. From Structure to Function. A Comprehensive Compendium of Tools to Unveil Protein Domains and Understand their Role in Cytokinesis

Sergio Rincon and Anne Paoletti