Wound Regeneration and Repair: Methods and Protocols

ISBN-13: 9781493959815 / Angielski / Miękka / 2016 / 585 str.

Robert G. Gourdie; Tereance A. Myers

Opis

This book details classical and cutting-edge methods for studying wound healing and regeneration. It includes a detailed list of the necessary materials and reagents for each procedure and step-by-step, reproducible laboratory protocols.

Spis treści

Part I: Tissue Engineered Wound Repair Models

1. An In Vivo Model System for Evaluation of the Host Response to Biomaterials                        

Brian Sicari, Neill Turner, and Stephen Badylak

 

2. Urothelial Cell Culture

Yuanyuan Zhang and Anthony Atala

 

3. Cell Populated Collagen Lattices Contraction Model for the

Investigation of Fibroblast Collagen Interactions

H Paul Ehrlich, and Kurtis E Moyer,

 

4. A Tissue-engineered Corneal Wound Healing Model for the Characterization of Reepithelialization

Karine Zaniolo, Patrick Carrier, Sylvain L. Guérin, François A. Auger and Lucie Germain

 

 

Part II: Stem Cells and Would Repair

5. Adult Stem Cells in Small Animal Wound Healing Models

Nauta, Allison C., Gurtner, Geoffrey C., Longaker, and  Michael T.,

 

6. Novel Animal Models for Tracking the Fate and Contributions of Bone Marrow Derived Cells in Diabetic Healing

Robert C. Caskey and Kenneth W. Liechty

 

7.

 

8. Cell-Based Therapies for Myocardial Repair: Emerging Role for Bone Marrow Derived Mesencymal Stem Cells (MSCs) in the Treatment of the Chronically Injured Heart

Jose S. Da Silva and Joshua M. Hare

 

9. A Model System for Primary Abdominal Closures

Michael J. Yost, Mary O. Morales, Veronica Rodriguez-Rivera, Eric M. Yost, Louis Terracio and Stephen A. Fann

 

Part III: Skin and Corneal Wound Repair Models

10.  Alternatives for Animal Wound Model Systems

Phil Stephens, Matthew Caley, and Matthew Peake

 

11. Novel Methods for the Investigation of Human Hypertrophic Scarring and other Dermal Fibrosis

Dariush Honardoust^, Peter Kwan, Moein Momtazi, Jie Ding , and Edward E Tredget

 

 

12. Study of the Human Chronic Wound Tissue: Addressing Logistic Barriers and Productive use of Laser Capture Microdissection

Sashwati Roy and Chandan K Sen

 

 

13. The Wound Watch: An Objective Staging System for Wounds in the Diabetic (db/db) Mouse Model

G. Pietramaggiori, , S. Scherer, and  DP. Orgill,

 

14. Human Ex vivo Wound Healing Model

Stojadinovic Olivera and Marjana Tomic – Canic

 

15. Murine Models of Human Wound Healing

Jerry S. Chen, Michael T. Longaker, and Geoffrey C. Gurtner

 

 

Part IV: Cardiovascular Wound Healing Models

16. A Corneal Scarring Model

Daniel J. Gibson and Gregory S. Schultz

 

17. A Novel and Efficient Model of Coronary Artery Ligation in the Mouse

Erhe Gao and Walter J. Koch

 

18. Cardiac Wound Healing Post-Myocardial Infarction: A Novel Method to Target Extracellular Matrix Remodeling in the Left Ventricle

Rogelio Zamilpa, Jianhua Zhang, Ying Ann Chiao, Lisandra de Castro Bras, Ganesh Halade, Yonggang Ma,  Sander O. Hacker, and Merry L. Lindsey

 

19. Injury Models to Study Cardiac Remodeling in the Mouse: Myocardial Infarction and Ischemia-reperfusion

Daniel J. Luther, Charles K. Thodeti, and J. Gary Meszaros

 

20. Cryoinjury Models of the Adult and Neonatal Mouse Heart for Studies of Scarring and Regeneration

Erik G. Strungs, Emily L. Ongstad, Michael P. O’Quinn, Joseph A. Palatinus, L. Jane Jourdan, and Robert G. Gourdie

 

21. Targeting Wnt Signaling to Improve Wound Healing after Myocardial Infarction

Evangelos P. Daskalopoulos, Ben J.A. Janssen and W. Matthijs Blankesteijn

 

22. Vascular Connexins in Restenosis after Balloon Injury

Sandrine Morel and Brenda R. Kwak

 

 

Part V: Non Mammalian and Mammalian Models of Regenerative Repair

23. Gain-of-function Assays in the Axolotl (Ambystoma mexicanum) to Identify Signaling Pathways that Induce and Regulate Limb Regeneration

Jangwoo Lee, Cristian Aguilar and David Gardiner

 

24. The Mouse Digit Tip: from Wound Healing to Regeneration

Jennifer Simkin, Manjong Han, Ling Yu, Mingquan Yan, and Ken Muneoka

 25. Lower Vibrissa Follicle Amputation: A Mammalian Model of RegenerationJames M. Waters and Allison J. Cowin

 

26. Using Drosophila Larvae to Study Epidermal Wound Closure and Inflammation

Sirisha Burra, Yan Wang, Amanda R. Brock, and Michael J. Galko

 

27.  Zebrafish Cardiac Injury and Regeneration Models: A Non-invasive and Invasive in vivo Model of Cardiac Regeneration

Michael S. Dickover, Ruilin Zhang, Peidong Han, and Neil C. Chi

 

 

Part VI: Wound Healing Cell Biology

28. Quantifying Alterations in Cell Migration: Tracking Fluorescently Tagged Migrating Cells by FACs and Live Imaging

Rachael Z. Murray

 

29. Examining the Role of Mast Cells in Fetal Wound Healing using Cultured Cells in vitro

Brian C. Wulff and Traci A. Wilgus

 

30. Assessing Macrophage Phenotype During Tissue Repair

Timothy J. Koh, Margaret L. Novak, and Rita E. Mirza

 

31. The use of Connexin-Based Therapeutic Approaches to Target Inflammatory Diseases

Simon J O’Carroll, David L Becker, Joanne O Davidson, Alistair J Gunn, Louise FB Nicholson, and  Colin R Green

 

Part VII: Commercialization of Wound Repair and Regenerative Discoveries

32. Commercialization: Patenting and Licensing in Wound Healing and Regenerative Biology

W.Chip. Hood and David Huizenga

 33. Translational Strategies for the Development of a Wound Healing Technology (idea) from Bench to Bedside

Gautam S. Ghatnekar  and Tuan A. Elstrom

 

Blurb

In Wound Regeneration and Repair: Methods and Protocols, expert researchers in the field detail classical and cutting-edge methods for studying wound healing and regeneration. These techniques include cellular and molecular methods, genetic approaches, surgical procedures, clinical advances, drug discovery and delivery modalities, animal and humanized models and new applications in the treatment of pathological wounds in a variety of organs and tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include an introduction to their respective topics, a detailed list of the necessary materials and reagents for each procedure, step-by-step, reproducible laboratory protocols, and a set notes, developed by the authors, for troubleshooting and avoiding known pitfalls.

 

 

Authoritative and practical, Wound Regeneration and Repair: Methods and Protocols seeks to aid scientists and entrepreneurs in their further study of technologies, models, techniques, and critical new areas and approaches to clinical and commercial translation of research.