Wound Regeneration: Methods and Protocols

ISBN-13: 9781071608470 / Angielski / Miękka / 2021 / 207 str.

Hiranmoy Das

Spis treści

1. Investigating Epidermal Interactions through an In Vivo Cutaneous Wound Healing Assay

            John L. Zemkewicz, Racheal G. Akwii, Constantinos M. Mikelis, and Colleen L. Doçi

 

2. A Murine Incisional Fetal Wound Healing Model to Study Scarless and Fibrotic Skin Repair

            Traci A. Wilgus

 

3. Development of Cutaneous Wound in Diabetic Immunocompromised Mice and Use of Dental Pulp-Derived Stem Cell Product for Healing

            Carl Greene and Hiranmoy Das

 

4. Musculoskeletal Tissue Engineering Using Fibrous Biomaterials

            George Tan, Yingge Zhou, and Dilshan Sooriyaarachchi

 

5. Cutaneous Wound Generation in Diabetic NOD/SCID Mice and the Use of Nanofiber-Expanded Hematopoietic Stem Cell Therapy

            Sarah Anderson and Hiranmoy Das

 

6. Contusion Rodent Model of Traumatic Brain Injury: Controlled Cortical Impact

            Marie-Line Fournier, Tifenn Clément, Justine Aussudre, Nikolaus Plesnila, André Obenaus, and Jérôme Badaut

 

7. Evaluation of MicroRNA Therapeutic Potential Using the Mouse In Vivo and Human Ex Vivo Wound Models

            Xi Li and Ning Xu Landén

 

8. Cellular Migration Assay: An In Vitro Technique to Simulate the Wound Repair Mechanism

            A.K.M. Nawshad Hossian and George Mattheolabakis

 

9. In Vivo Ear Sponge Lymphangiogenesis Assay

            Racheal G. Akwii, Md S. Sajib, Fatema T. Zahra, Hanumantha R. Madala, Kalkunte S. Srivenugopal, and Constantinos M. Mikelis

 

10. Identification of Rho-GEF and RhoA Activation by Pull-Down Assays

            Md S. Sajib, Fatema T. Zahra, Racheal G. Akwii, and Constantinos M. Mikelis

 

11. Wound Matrix Stiffness Imposes on Macrophage Activation

            Pu Duann and Pei-Hui Lin

 

12. Generation of Acute Hind Limb Ischemia in NOD/SCID Mice and Treatment with Nanofiber-Expanded CD34⁺ Hematopoietic Stem Cells

            Derek Barthels and Hiranmoy Das

 

13. Electrospun Aligned Co-Axial Nanofibrous Scaffold for Cardiac Repair

            Divya Sridharan, Arunkumar Palaniappan, Britani N. Blackstone, Heather Powell, and Mahmood Khan

 

14. Generation of Myocardial Ischemic Wounds and Healing with Stem Cells

            Daniela Rolph and Hiranmoy Das

 

15. Corneal Repair Models in Mice: Epithelial/Mechanical Versus Stromal/Chemical Injuries

            Peipei Pan and Matilda F. Chan

 

16. Topical Adoptive Transfer of Plasmacytoid Dendritic Cells for Corneal Wound Healing

            Arsia Jamali, Brendan M. Kenyon, Gustavo Ortiz, Betul N. Bayraktutar, Victor G. Sendra, and Pedram Hamrah

 

17. Murine Corneal Epithelial Wound Modeling

            Dhara Shah and Vinay Kumar Aakalu

 

18. Infection-Induced Porcine Ex Vivo Corneal Wound Model to Study the Efficacy of Herpes Simplex Virus-1 Entry and Replication Inhibitors

            Tejabhiram Yadavalli, Raghuram Koganti, and Deepak Shukla

Blurb

This detailed book explores a diverse range of topics related to wound healing. Some areas include wound generation as a tool to study cellular interactions and growth, mechanisms by which different tissues regenerate, ischemic wounds and their treatment, repairing corneal wound, as well as a number of emerging technologies to help promote wound regeneration, such as techniques with miRNA, nanomaterials, biomaterials, and stem cell therapies. Written for the highly successful Methods in Molecular Biology series, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Wound Regeneration: Methods and Protocols serves as a vital collection of wound model development and regeneration studies that will be of great use in both the academic and industrial fields of research.