Wolbachia: Methods and Protocols

ISBN-13: 9781071635520 / Angielski

Ann M. Fallon

Spis treści

 1. Wolbachia:  Advancing into a second century

Ann M Fallon  

 

Part 1.  Wolbachia-Mediated Phenotypes

 

2. Investigation of cytoplasmic incompatibility patterns in Culex pipiens: from field samples to laboratory isofemale lines

Alice Namias, Célestine Atyame, Nicole Pasteur, Mathieu Sicard, and Mylène Weill

 

3. Maintenance and Evaluation of Wolbachia Male-Killers of Dipterans

Margaret E. Schedl, Ngoc Huan Nguyen, Robert L. Unckless, and Jessamyn I. Perlmutter

 

4. Examining Wolbachia-Induced Parthenogenesis in Hymenoptera

Laura C. Fricke and Amelia RI Lindsey

 

5. Pillbug: a toolbox for feminisation

Joanne Bertaux, Maryline Raimond, and Pierre Grève

 

6. Detection, Quantification and Elimination of Wolbachia in Bed Bugs,

Madhavi L. Kakumanu, Mauri L. Hickin, and Coby Schal

 

7. Detection and analysis of Wolbachia in plant-parasitic nematodes and insights into Wolbachia evolution

Amandeep Kaur and Amanda M. V. Brown

 

Part 2.  Wolbachia in cell culture

 

8. Developing Wolbachia-infected cell lines from insects,

Mukund Madhav and Peter James

 

9. Use of antibiotics to eliminate Wolbachia from mosquitos and cell culture

Cameron Bishop and Sassan Asgari

 

10. Preparation of infectious Wolbachia from a mosquito cell line

Ann M Fallon

 

Part III:  Wolbachia for vector control

 

11. Wolbachia transinfection via embryonic microinjection

Meichun Zhang and Zhiyong Xi

 

12. Measuring host fitness effects and transmission of Wolbachia strains in Aedes aegypti mosquitoes,

Perran A. Ross

 

13. Detection of natural Wolbachia strains in Anopheles mosquitoes

Thomas Walker

 

14. Procedures for detection of Wolbachia-conferred antiviral protection in Drosophila melanogaster

Ewa Chrostek

 

16. Validating a mitochondrial sweep accompanying the rapid spread of a maternally-inherited symbiont

Anne Duplouy

 

Part 4:  Investigating Wolbachia and its effects

 

16. Screening host genomic data for Wolbachia infections

Federica Valerio^, Victoria G. Twort, and Anne Duplouy

 

17.  A worked example of screening genomic material for the presence of Wolbachia infection

Federica Valerio^, Victoria G. Twort, and Anne Duplouy

 

18. Use of Drosophila Transgenics to Identify Functions for Symbiont Effectors

Carai T. Cortez, Richard O. Murphy, Isabella M. Owens, and John F. Beckmann

 

19. Using Baker’s Yeast to Determine Functions of Novel Wolbachia (and other prokaryotic) Effectors  

Richard O. Murphy and John F. Beckmann

 

20. Isolation of Phage WO Particles from Wolbachia-infected Arthropods

Sarah R. Bordenstein

 

21. A light in the dark: Uncovering Wolbachia-host interactions using fluorescence imaging

Laura Renee Serbus

Blurb

This book provides insight into the biology of Wolbachia with a particular focus on methodology.  Chapters guide readers through reproductive phenotypes caused by Wolbachia, survival and replication of Wolbachia in insect cell lines, artificial introduction of Wolbachia into novel hosts, and analysis of Wolbachia gene functions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.