Western Blotting Guru

ISBN-13: 9780128135372 / Angielski / Miękka / 2017 / 100 str.

Ayaz Najafov; Gerta Hoxhaj

Opis

Western Blotting Guru provides researchers in molecular biology with a handy reference for approaching and solving challenging problems associated with immunoblotting setup and optimization. As a laboratory guide, it emphasizes the technical aspects of efficiently employing immunoblotting as a tool in molecular biology laboratories. The book covers the basic science underlying immunoblotting and detailed description of the method parameters, followed by good benchtop practices, tips and tricks for obtaining high-quality data and a detailed troubleshooting guide addressing a variety of problem types.

• Provides a benchtop reference that every molecular biologists will use to design, optimize, troubleshoot and analyze their immunoblotting experiments
• Contains unique good practices and tips that are indispensable for the beginner and expert alike
• Features special cases with applications of immunoblotting optimization
• Includes detailed appendices with tables, figures and key protocols
• Provides troubleshooting tips for various types of modifications of standard protocols
• Organized as a systematic, concentrated resource to save time when addressing an immunoblotting problem

Spis treści

1. Introduction2. Procedure3. Good Practices4. Troubleshooting5. Tips and Tricks6. Special Cases

O autorze

Ayaz was born in Baku, Azerbaijan. He obtained his BSc and MSc degrees in Molecular Biology and Genetics from Bosphorus University, Istanbul, Turkey and got a 2-year training in Biochemistry as an intern at Baylor College of Medicine, Houston, Texas, USA. Ayaz pursued his PhD in Biochemistry at MRC Protein Phosphorylation Unit at Dundee University, Scotland, UK in Prof. Dario Alessi's lab. Ayaz is currently a postdoctoral fellow in Prof. Junying Yuan's lab at Harvard Medical School, Cell Biology Department. Gerta was born in Fier, Albania. She obtained her BSc degrees in Molecular Biology and Genetics, as well as a second major in Chemistry from Bosphorus University, Istanbul, Turkey. Gerta pursued her PhD in Biochemistry at MRC Protein Phosphorylation Unit at Dundee University, Scotland, UK in Prof. Carol MacKintosh's lab. Gerta is currently a postdoctoral fellow in Prof. Brendan Mannings's lab at Harvard T.H. Chan School of Public Health, Department of Genetics and Complex Diseases.