West Nile Virus: Methods and Protocols

ISBN-13: 9781493936687 / Angielski / Twarda / 2016 / 295 str.

Tonya M. Colpitts

Opis

Chapters in the book cover many facets ofWNV, such as propagation and titration of WNV in vero cells, examination of WNVneuroinvasion and neuropathogenesis in the central nervous system of a murinemodel, field surveillance methods for WNV, and in vitro and in vivoblood-brain barrier models to study WNV pathogenesis.

Spis treści

1. A Brief Review of West Nile Virus Biology
Berlin Londono-Renteria and Tonya M. Colpitts

2.  Overview of West Nile Virus Transmission and Epidemiology
Andrea Troupin and Tonya M. Colpitts

3. Propagation and Titration of West Nile Virus on Vero Cells
Alexander J. McAuley and David W. C. Beasley

4. Generating West Nile Virus from an Infectious Clone
Rianna Vandergasst and Brenda L. Frederickson

5. Functional Analysis of West Nile Virus Proteins in Human Cells
Pakieli H. Kaufusi, Alanna Tseng, and Vivek R. Nerurkar

6. Lessons from the Murine Models of West Nile Virus Infection
Brenna McGruder, Vandana Saxena, and Tian Wang

7. Exploration of West Nile Virus Infection in Mouse Models
Penghua Wang

8. Examination of West Nile Virus Neuroinvasion and Neuropathogenesis in the Central Nervous System of a Murine Model
Hameeda Sultana

9.  In Vitro and In Vivo Blood-Brain Barrier Models to Study West Nile Virus Pathogenesis
Mukesh Kumar and Vivek R. Nerurkar

10. Methodology for Identifying Host Factors Involved in West Nile Virus Infection
Manoj N. Krishnan

11. ELISA and Neutralization Methods to Measure Anti-West Nile Virus Antibody Responses
Brian M. Friedrich and David W. C. Beasley

12. Safe Handling of West Nile Virus in the Insectary
Dexter R. McKellar and Michael J. Conway

13. Techniques for Experimental Infection of Mosquitoes with West Nile Virus
Yang Liu and Gong Cheng

14.  Field Surveillance Methods for West Nile Virus
Samuel B. Jameson, and Dawn M. Wesson

15. Detection Protocols for West Nile Virus in Mosquitoes, Birds, and Non-Human Mammals
Elizabeth B. Kauffman, Mary A. Franke, and Laura D. Kramer

16. Field Methods and Sample Collection Techniques for the Surveillance of West Nile Virus in Avian Hosts
Sarah S. Wheeler, Walter M. Boyce, and William K. Reisen 

17. Statistical Tools for the Interpretation of Enzootic West Nile Virus Transmission Dynamics
Kevin A. Caillouët and Suzanne Robertson

18. Role of Immune Aging in Susceptibility to West Nile Virus
Yi Yao and Ruth R. Montgomery
19. An Overview of Current Approaches Toward the Treatment and Prevention of West Nile Virus Infection
Dhiraj Acharya and Fengwei Bai

Blurb

This volume is a collection of research methods, techniques, and approaches to investigate the molecular biology of West Nile Virus (WNV). Chapters in the book cover many facets of WNV, such as propagation and titration of WNV in vero cells, examination of WNV neuroinvasion and neuropathogenesis in the central nervous system of a murine model, field surveillance methods for WNV, and in vitro and in vivo blood-brain barrier models to study WNV pathogenesis. A brief introduction, along with a discussion on WNV laboratory safety are also included. Written in the highly successful Methods in Molecular Biology series format, chapters includes introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and thorough, West Nile Virus will interest virologists, molecular biologists, microbiologists, and other scientists working with WNV. This book is a comprehensive guide for those conducting research in the field of WNV biology, including life cycles, pathogenesis, impact of infection, and possible treatment/prevention development.