1. Automated large volume sample preparation for vEM Claudia S. Lopez 2. Resin comparison for Serial Block Face Scanning Volume Electron Microscopy Anneke Kremer, Saskia Lippens and Chris Geurin 3. Immunolabelling for SBF-SEM Alice Liang 4. Volume Electron Microscopy in Plants Kirk James Czymmek 5. Serial section electron tomography Andrea Raimondi 6. Automated Tape Collecting Ultramicrotomy (ATUM) for targeting neuropathology Martina Schifferer 7. Array tomography Jemima J. Burden and Ian White 8. Mitochondria morphometry in 3d datasets of mouse brain obtained with serial block-face Scanning Electron Microscopy Christel Genoud, Jean-Yves Chatton and Wei Jiao 9. Serial Block-face Scanning Electron Microscopy of Schmidtea mediterranea Stephanie Nowotarski 10. Correlative multiscale microCT-SBF-SEM imaging of resin-embedded tissue Rohan M. Lewis, Orestis L. Katsamenis and Pascale Chavatte-Palmer 11. Methods of enhanced FIB-SEM sample preparation and image acquisition Song Pang 12. Functional characterization of endo-lysosomal compartments by correlative live-cell and volume electron microscopy Nalan Liv 13. Cryo FIB-SEM imaging Michele C. Darrow and James Naismith 14. Toward scalable reuse of vEM data: OME-Zarr to the rescue Norman Rzepka, Joshua Allen Moore and John Bogovic 15. Public archiving of vEM data Gerard J. Kleywegt and Ardan Patwardhan