Visualization Techniques: From Immunohistochemistry to Magnetic Resonance Imaging

ISBN-13: 9781617798962 / Angielski / Twarda / 2012 / 316 str.

Emilio Badoer

Opis

Visualization of chemicals in tissues has seen incredible advances in the past several years. Visualization Techniques: From Immunohistochemistry to Magnetic Resonance Imaging provides practical advice from experts in the field as well as an excellent overview of some of the most important recent advances in visualization. This timely volume explores topics from immunohistochemistry for multiple neurochemicals, detecting expression levels of neurochemicals, following cellular processes and ionic movement, identifying polysynaptic pathways subserving physiological responses to identifying functional changes in vivo. Written for the popular Neuromethods series, this work includes the kind of detailed description and implementation advice that is crucial for getting optimal results in the lab. Meticulous and concise, Visualization Techniques: From Immunohistochemistry to Magnetic Resonance Imaging will prove invaluable for scientists seeking to gain a greater understanding of the practical skills, strengths, and pitfalls that these wonderful and exciting visualization techniques provide.

Recenzje

From the reviews:

"This is an excellent overview of advances in visualization techniques in neuroscience, particularly at the molecular level. The book is written for neuroscientists, basic neuroresearchers, and students of medical science. It is also appropriate for clinical and academic specialists in neurobiology, neuropharmacology, neurology, neuro-oncology, neuroradiology, and neurosurgery. ... It is an inspiration for investigators interested in furthering their knowledge of the visualization of the nervous system not only in its entirety, but also at the neuronal level." (Ramsis Farid Ghaly, Doody's Book Reviews, August, 2012)

Spis treści

1. Multiple Immunohistochemical Labelling of Peripheral Neurons

            Ian L. Gibbins

 

2. Combined In Situ Hybridization and Immunohistochemistry in Tat Brain Tissue Using Digoxigenin Labelled Riboprobes

            Natasha N. Kumar, Belinda R. Bowman and Ann K. Goodchild

 

3. In Situ Hybridization within the CNS Tissue: Combining In Situ Hybridization with Immunofluorescence

            Dominic Bastien and Steve Lacroix

 

4. Visualizing GABA_B Receptor Internalization and Intracellular Trafficking

            Paola Ramoino, Paolo Bianchini, Alberto Diaspro, and Cesare Usai

 

5. Using Total Internal Reflection Fluorescence Microscopy  (TIRFM) to Visualize Insulin Action

            James G. Burchfield, Jamie A. Lopez, and William E. Hughes

 

6. Live-Cell Quantification of Mitochondrial Functional Parameters

            Marco Nooteboom, Marleen Forkink, Peter H.G.M. Willems, and Werner J.H. Koopman

 

7. Functional Imaging Using Two-Photon Microscopy in Living Tissue

            Ivo Vanzetta, Thomas Deneux, Attila Kaszás, Gergely Katona, and Balazs Rozsa

 

8. Calcium Imaging Techniques In Vitro to Explore the Role of Dendrites in Signaling Physiological Action Potential Patterns

            Audrey Bonnan, Benjamin Grewe, and Andreas Frick

 

9. Juxtacellular Labeling in Combination with Other Histological Techniques to Determine Phenotype of Physiologically Identified Neurons

            Ruth L. Stornetta

 

10. Visualization of Activated Neurons Involved in Endocrine and Dietary Pathways Using GFP-Expressing Mice

            Rim Hassouna, Odile Viltart, Lucille Tallot, Karine Bouyer, Catherine Videau, Jacques Epelbaum, Virginie Tolle, and Emilio Badoer

 

11. Use and Visualization of Neuroanatomical Viral Transneuronal Tracers

            J. Patrick Card and Lynn W. Enquist

 

12. Visualization of Thermal Changes in Freely Moving Animals

            Daniel M.L. Vianna and Pascal Carrive

 

13. Perfusion Magnetic Resonance Imaging Quantification in the Brain

            Fernando Calamante

            Ian L. Gibbins

 

2. Combined In Situ Hybridization and Immunohistochemistry in Tat Brain Tissue Using Digoxigenin Labelled Riboprobes

            Natasha N. Kumar, Belinda R. Bowman and Ann K. Goodchild

 

3. In Situ Hybridization within the CNS Tissue: Combining In Situ Hybridization with Immunofluorescence

            Dominic Bastien and Steve Lacroix

 

4. Visualizing GABA_B Receptor Internalization and Intracellular Trafficking

            Paola Ramoino, Paolo Bianchini, Alberto Diaspro, and Cesare Usai

 

5. Using Total Internal Reflection Fluorescence Microscopy  (TIRFM) to Visualize Insulin Action

            James G. Burchfield, Jamie A. Lopez, and William E. Hughes

 

6. Live-Cell Quantification of Mitochondrial Functional Parameters

            Marco Nooteboom, Marleen Forkink, Peter H.G.M. Willems, and Werner J.H. Koopman

 

7. Functional Imaging Using Two-Photon Microscopy in Living Tissue

            Ivo Vanzetta, Thomas Deneux, Attila Kaszás, Gergely Katona, and Balazs Rozsa

 

8. Calcium Imaging Techniques In Vitro to Explore the Role of Dendrites in Signaling Physiological Action Potential Patterns

            Audrey Bonnan, Benjamin Grewe, and Andreas Frick

 

9. Juxtacellular Labeling in Combination with Other Histological Techniques to Determine Phenotype of Physiologically Identified Neurons

            Ruth L. Stornetta

 

10. Visualization of Activated Neurons Involved in Endocrine and Dietary Pathways Using GFP-Expressing Mice

            Rim Hassouna, Odile Viltart, Lucille Tallot, Karine Bouyer, Catherine Videau, Jacques Epelbaum, Virginie Tolle, and Emilio Badoer

 

11. Use and Visualization of Neuroanatomical Viral Transneuronal Tracers

            J. Patrick Card and Lynn W. Enquist

 

12. Visualization of Thermal Changes in Freely Moving Animals

            Daniel M.L. Vianna and Pascal Carrive

 

13. Perfusion Magnetic Resonance Imaging Quantification in the Brain

            Fernando Calamante

            Natasha N. Kumar, Belinda R. Bowman and Ann K. Goodchild

 

3. In Situ Hybridization within the CNS Tissue: Combining In Situ Hybridization with Immunofluorescence

            Dominic Bastien and Steve Lacroix

 

4. Visualizing GABA_B Receptor Internalization and Intracellular Trafficking

            Paola Ramoino, Paolo Bianchini, Alberto Diaspro, and Cesare Usai

 

5. Using Total Internal Reflection Fluorescence Microscopy  (TIRFM) to Visualize Insulin Action

            James G. Burchfield, Jamie A. Lopez, and William E. Hughes

 

6. Live-Cell Quantification of Mitochondrial Functional Parameters

            Marco Nooteboom, Marleen Forkink, Peter H.G.M. Willems, and Werner J.H. Koopman

 

7. Functional Imaging Using Two-Photon Microscopy in Living Tissue

            Ivo Vanzetta, Thomas Deneux, Attila Kaszás, Gergely Katona, and Balazs Rozsa

 

8. Calcium Imaging Techniques In Vitro to Explore the Role of Dendrites in Signaling Physiological Action Potential Patterns

            Audrey Bonnan, Benjamin Grewe, and Andreas Frick

 

9. Juxtacellular Labeling in Combination with Other Histological Techniques to Determine Phenotype of Physiologically Identified Neurons

            Ruth L. Stornetta

 

10. Visualization of Activated Neurons Involved in Endocrine and Dietary Pathways Using GFP-Expressing Mice

            Rim Hassouna, Odile Viltart, Lucille Tallot, Karine Bouyer, Catherine Videau, Jacques Epelbaum, Virginie Tolle, and Emilio Badoer

 

11. Use and Visualization of Neuroanatomical Viral Transneuronal Tracers

            J. Patrick Card and Lynn W. Enquist

 

12. Visualization of Thermal Changes in Freely Moving Animals

            Daniel M.L. Vianna and Pascal Carrive

 

13. Perfusion Magnetic Resonance Imaging Quantification in the Brain

            Fernando Calamante

            Paola Ramoino, Paolo Bianchini, Alberto Diaspro, and Cesare Usai

 

5. Using Total Internal Reflection Fluorescence Microscopy  (TIRFM) to Visualize Insulin Action

            James G. Burchfield, Jamie A. Lopez, and William E. Hughes

 

6. Live-Cell Quantification of Mitochondrial Functional Parameters

            Marco Nooteboom, Marleen Forkink, Peter H.G.M. Willems, and Werner J.H. Koopman

 

7. Functional Imaging Using Two-Photon Microscopy in Living Tissue

            Ivo Vanzetta, Thomas Deneux, Attila Kaszás, Gergely Katona, and Balazs Rozsa

 

8. Calcium Imaging Techniques In Vitro to Explore the Role of Dendrites in Signaling Physiological Action Potential Patterns

            Audrey Bonnan, Benjamin Grewe, and Andreas Frick

 

9. Juxtacellular Labeling in Combination with Other Histological Techniques to Determine Phenotype of Physiologically Identified Neurons

            Ruth L. Stornetta

 

10. Visualization of Activated Neurons Involved in Endocrine and Dietary Pathways Using GFP-Expressing Mice

            Rim Hassouna, Odile Viltart, Lucille Tallot, Karine Bouyer, Catherine Videau, Jacques Epelbaum, Virginie Tolle, and Emilio Badoer

 

11. Use and Visualization of Neuroanatomical Viral Transneuronal Tracers

            J. Patrick Card and Lynn W. Enquist

 

12. Visualization of Thermal Changes in Freely Moving Animals

            Daniel M.L. Vianna and Pascal Carrive

 

13. Perfusion Magnetic Resonance Imaging Quantification in the Brain

            Fernando Calamante

            James G. Burchfield, Jamie A. Lopez, and William E. Hughes

 

6. Live-Cell Quantification of Mitochondrial Functional Parameters

            Marco Nooteboom, Marleen Forkink, Peter H.G.M. Willems, and Werner J.H. Koopman

 

7. Functional Imaging Using Two-Photon Microscopy in Living Tissue

            Ivo Vanzetta, Thomas Deneux, Attila Kaszás, Gergely Katona, and Balazs Rozsa

 

8. Calcium Imaging Techniques In Vitro to Explore the Role of Dendrites in Signaling Physiological Action Potential Patterns

            Audrey Bonnan, Benjamin Grewe, and Andreas Frick

 

9. Juxtacellular Labeling in Combination with Other Histological Techniques to Determine Phenotype of Physiologically Identified Neurons

            Ruth L. Stornetta

 

10. Visualization of Activated Neurons Involved in Endocrine and Dietary Pathways Using GFP-Expressing Mice

            Rim Hassouna, Odile Viltart, Lucille Tallot, Karine Bouyer, Catherine Videau, Jacques Epelbaum, Virginie Tolle, and Emilio Badoer

 

11. Use and Visualization of Neuroanatomical Viral Transneuronal Tracers

            J. Patrick Card and Lynn W. Enquist

 

12. Visualization of Thermal Changes in Freely Moving Animals

            Daniel M.L. Vianna and Pascal Carrive

 

13. Perfusion Magnetic Resonance Imaging Quantification in the Brain

            Fernando Calamante

            Marco Nooteboom, Marleen Forkink, Peter H.G.M. Willems, and Werner J.H. Koopman

 

7. Functional Imaging Using Two-Photon Microscopy in Living Tissue

            Ivo Vanzetta, Thomas Deneux, Attila Kaszás, Gergely Katona, and Balazs Rozsa

 

8. Calcium Imaging Techniques In Vitro to Explore the Role of Dendrites in Signaling Physiological Action Potential Patterns

            Audrey Bonnan, Benjamin Grewe, and Andreas Frick

 

9. Juxtacellular Labeling in Combination with Other Histological Techniques to Determine Phenotype of Physiologically Identified Neurons

            Ruth L. Stornetta

 

10. Visualization of Activated Neurons Involved in Endocrine and Dietary Pathways Using GFP-Expressing Mice

            Rim Hassouna, Odile Viltart, Lucille Tallot, Karine Bouyer, Catherine Videau, Jacques Epelbaum, Virginie Tolle, and Emilio Badoer

 

11. Use and Visualization of Neuroanatomical Viral Transneuronal Tracers

            J. Patrick Card and Lynn W. Enquist

 

12. Visualization of Thermal Changes in Freely Moving Animals

            Daniel M.L. Vianna and Pascal Carrive

 

13. Perfusion Magnetic Resonance Imaging Quantification in the Brain

            Fernando Calamante

            Ivo Vanzetta, Thomas Deneux, Attila Kaszás, Gergely Katona, and Balazs Rozsa

 

8. Calcium Imaging Techniques In Vitro to Explore the Role of Dendrites in Signaling Physiological Action Potential Patterns

            Audrey Bonnan, Benjamin Grewe, and Andreas Frick

 

9. Juxtacellular Labeling in Combination with Other Histological Techniques to Determine Phenotype of Physiologically Identified Neurons

            Ruth L. Stornetta

 

10. Visualization of Activated Neurons Involved in Endocrine and Dietary Pathways Using GFP-Expressing Mice

            Rim Hassouna, Odile Viltart, Lucille Tallot, Karine Bouyer, Catherine Videau, Jacques Epelbaum, Virginie Tolle, and Emilio Badoer

 

11. Use and Visualization of Neuroanatomical Viral Transneuronal Tracers

            J. Patrick Card and Lynn W. Enquist

 

12. Visualization of Thermal Changes in Freely Moving Animals

            Daniel M.L. Vianna and Pascal Carrive

 

13. Perfusion Magnetic Resonance Imaging Quantification in the Brain

            Fernando Calamante

            Audrey Bonnan, Benjamin Grewe, and Andreas Frick

 

9. Juxtacellular Labeling in Combination with Other Histological Techniques to Determine Phenotype of Physiologically Identified Neurons

            Ruth L. Stornetta

 

10. Visualization of Activated Neurons Involved in Endocrine and Dietary Pathways Using GFP-Expressing Mice

            Rim Hassouna, Odile Viltart, Lucille Tallot, Karine Bouyer, Catherine Videau, Jacques Epelbaum, Virginie Tolle, and Emilio Badoer

 

11. Use and Visualization of Neuroanatomical Viral Transneuronal Tracers

            J. Patrick Card and Lynn W. Enquist

 

12. Visualization of Thermal Changes in Freely Moving Animals

            Daniel M.L. Vianna and Pascal Carrive

 

13. Perfusion Magnetic Resonance Imaging Quantification in the Brain

            Fernando Calamante

            Ruth L. Stornetta

 

10. Visualization of Activated Neurons Involved in Endocrine and Dietary Pathways Using GFP-Expressing Mice

            Rim Hassouna, Odile Viltart, Lucille Tallot, Karine Bouyer, Catherine Videau, Jacques Epelbaum, Virginie Tolle, and Emilio Badoer

 

11. Use and Visualization of Neuroanatomical Viral Transneuronal Tracers

            J. Patrick Card and Lynn W. Enquist

 

12. Visualization of Thermal Changes in Freely Moving Animals

            Daniel M.L. Vianna and Pascal Carrive

 

13. Perfusion Magnetic Resonance Imaging Quantification in the Brain

            Fernando Calamante

 

10. Visualization of Activated Neurons Involved in Endocrine and Dietary Pathways Using GFP-Expressing Mice

            Rim Hassouna, Odile Viltart, Lucille Tallot, Karine Bouyer, Catherine Videau, Jacques Epelbaum, Virginie Tolle, and Emilio Badoer

 

11. Use and Visualization of Neuroanatomical Viral Transneuronal Tracers

            J. Patrick Card and Lynn W. Enquist

 

12. Visualization of Thermal Changes in Freely Moving Animals

            Daniel M.L. Vianna and Pascal Carrive

 

13. Perfusion Magnetic Resonance Imaging Quantification in the Brain

            Fernando Calamante

            Rim Hassouna, Odile Viltart, Lucille Tallot, Karine Bouyer, Catherine Videau, Jacques Epelbaum, Virginie Tolle, and Emilio Badoer

 

11. Use and Visualization of Neuroanatomical Viral Transneuronal Tracers

            J. Patrick Card and Lynn W. Enquist

 

12. Visualization of Thermal Changes in Freely Moving Animals

            Daniel M.L. Vianna and Pascal Carrive

 

13. Perfusion Magnetic Resonance Imaging Quantification in the Brain

            Fernando Calamante

            J. Patrick Card and Lynn W. Enquist

 

12. Visualization of Thermal Changes in Freely Moving Animals

            Daniel M.L. Vianna and Pascal Carrive

 

13. Perfusion Magnetic Resonance Imaging Quantification in the Brain

            Fernando Calamante

            Daniel M.L. Vianna and Pascal Carrive

 

13. Perfusion Magnetic Resonance Imaging Quantification in the Brain

            Fernando Calamante

 

13. Perfusion Magnetic Resonance Imaging Quantification in the Brain

            Fernando Calamante

            Fernando Calamante