Virus Hybrids as Nanomaterials: Methods and Protocols

ISBN-13: 9781627037501 / Angielski / Twarda / 2013 / 235 str.

Baochuan Lin; Banahalli Ratna

Opis

In Virus Hybrids as Nanomaterials: Methods and Protocols expert researchers in the field detail many of the methods used to study virus for medial and nonmedical applications. These include methods and techniques for genetically engineering viruses for therapeutic purpose and vaccine production, chemically modified viruses for virus-templated nanoparticles production, and genetically engineered or chemically modified viral particles as imaging agents. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Virus Hybrids as Nanomaterials: Methods and Protocols seek to aid new researchers to get involved in this multidisciplinary area.

Spis treści

Part I: Virus Hybrids as Therapeutic/Vaccine Materials

1. Genetic Engineering and Chemical Conjugation of Potato Virus X

Karin L. Le, Kerstin Uhde-Holzem, Rainer Fischer, Ulrich Commandeur, Nicole F. Steinmetz

2. Genetically Engineering Adenoviral Vectors for Gene Therapy

Lynda Coughlan

3. Simulated Digestion for Testing the Stability of Edible Vaccine, Cucumber mosaic virus (CMV) Chimeric Particle Display Hepatitis C virus (HCV) Peptide

Vitti Antonella, Nuzzaci Maria, Condelli Valentina, Piazzolla Pasquale

4. Using Phage as a Platform to Select Cancer Cell Targeting Peptides

Xin Li, Chuanbin Mao

5. Bacteriophage T4 Capsid Packaging and Unpackaging of DNA and Proteins

Julienne M. Mullaney and Lindsay W. Black

Part II: Virus as building Block

6. Templated Mineralization by Charge-modified Cowpea Mosaic Virus

Alaa A. A. Aljabali and David J. Evans

7. Polyelectrolyte-modified Cowpea Mosaic Virus for the Synthesis of Gold Nanoparticles

Alaa A. A. Aljabali and David J. Evans

8. Tobacco Mosaic Virus Capsid Protein as Targets for the Self-assembly of Gold Nanoparticles

Omar K. Zahr, Amy Szuchmacher Blum

9. Tobacco Mosaic Virus Assembled High Aspect Ratio Surfaces

Adam D. Brown and James N. Culver

10. Phage as a Template to Grow Bone Mineral Nanocrystals

Binrui Cao, Hong Xu, and Chuanbin Mao

Part III: Virus Hybrids as Imaging Materials

11. Genetic Engineering and Characterisation of Cowpea Mosaic Virus Empty Virus-like Particles

Frank Sainsbury, Pooja Saxena, Alaa AA Aljabali, Keith Saunders, David J Evans, George P Lomonossoff

12. A Programmable Fluorescent Viral Nanoblock: Sensing Made Easy in a Single Step

Carissa M. Soto

13. Chemical Modification of the Inner and Outer Surfaces of Tobacco Mosaic Virus (TMV)

Michael A. Bruckman and Nicole F. Steinmetz

14. Engineered Bacteriophage T4 Nanoparticles for Cellular Imaging

Jinny L. Liu and Kelly L. Robertson

15. Phage-displayed Single Domain Antibodies as Recognition Elements

Ellen Goldman and Scott Walper

16. Molecular Targeted Viral Nanoparticles as Tools for Imaging Cancer

Cho, C.-F., Sourabh, S., Simpson, E.J., Steinmetz, N.F., Luyt, L.G., and Lewis, J.D.

Blurb

In Virus Hybrids as Nanomaterials: Methods and Protocols expert researchers in the field detail many of the methods used to study virus for medial and nonmedical applications.  These include methods and techniques for  genetically engineering viruses for therapeutic purpose and vaccine production, chemically modified viruses for virus-templated nanoparticles production, and genetically engineered or chemically modified viral particles as imaging agents. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Virus Hybrids as Nanomaterials: Methods and Protocols seeks to aid new researchers to get involved in this multidisciplinary area.