Vascular Morphogenesis: Methods and Protocols

ISBN-13: 9781071609187 / Angielski / Miękka / 2020 / 234 str.

Domenico Ribatti

Spis treści

1. Historical Overview of the In Vivo and In Vitro Angiogenesis Assays

            Anca Maria Cimpean and Marius Raica

 

2. The Fundamental Contribution of Judah Folkman in the Setting of Angiogenesis Assays

            Domenico Ribatti

 

3. Identification of Endothelial Cells and Their Progenitors

            Ellen Go and Mervin C. Yoder

 

4. In Vitro Co-Culture Assays of Angiogenesis

            Haoche Wei, Ananthalakshmy Sundararaman, Sarah Line Bring Truelsen, David Gurevich, Jacob Thastrup, and Harry Mellor

 

5. Endothelial Cells: Co-Culture Spheroids

            Janos M. Kanczler, Julia A. Wells, and Richard O.C. Oreffo

 

6. Microfluidic Device Setting by Co-Culturing Endothelial Cells and Mesenchymal Stem Cells

            Masafumi Watanabe and Ryo Sudo

 

7. Spatial Statistics-Based Image Analysis Methods for the Study of Vascular Morphogenesis

            Guidolin Diego, Tortorella Cinzia, and Domenico Ribatti

 

8. Studying Angiogenesis in the Rabbit Corneal Pocket Assay

            Lucia Morbidelli, Valerio Ciccone, and Marina Ziche

 

9. Avians as a Model System of Vascular Development

            Rieko Asai, Michael Bressan, and Takashi Mikawa

 

10. Dynamic Imaging of Mouse Embryos and Cardiac Development in Static Culture

            Andrew L. Lopez III and Irina V. Larina

 

11. High-Resolution Confocal Imaging of Pericytes in Human Fetal Brain Microvessels

            Mariella Errede, Francesco Girolamo, and Daniela Virgintino

 

12. Quantification of Tumor Vasculature by Analysis of Amount and Spatial Dispersion of Caliber-Classified Vessels

            Marco Righi, Marco Presta, and Arianna Giacomini

 

13. A Xenograft Model for Venous Malformation

            Jillian Goines and Elisa Boscolo

 

14. In Vivo Vascular Network Forming Assay

            Hwan D. Kim, Ruei-Zeng Lin, and Juan M. Melero-Martin

 

15. Assessment of Vascular Patterning in the Zebrafish

            Amber N. Stratman and Brant M. Weinstein

 

16. By the Skin of Your Teeth: A Subcutaneous Mouse Model to Study Pulp Regeneration

            Annelies Bronckaers, Petra Hilkens, Esther Wolfs, and Ivo Lambrichts

Blurb

This detailed new edition aims to provide a range of methods and protocols for studying vascular morphogenesis in vivo and in vitro to reflect up-to-date advances in the field. Of note, the book explores endothelial cell signaling is currently understood to promote fundamental cues for cell fate specification, embryo patterning, organ differentiation, and postnatal tissue remodeling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and timely, Vascular Morphogenesis: Methods and Protocols, Second Edition will serve as an ideal guide to this area of study for a broad audience amongst cell biologists, anatomists, pathologists, and physiologists.