Vascular Effects of Hydrogen Sulfide: Methods and Protocols

ISBN-13: 9781493995271 / Angielski / Twarda / 2019 / 221 str.

Jerzy Beltowski

Spis treści

1. Synthesis, Metabolism, and Signaling Mechanisms of Hydrogen Sulfide: An Overview

            Jerzy Bełtowski

 

2. Lanthionine and Other Relevant Sulfur Amino Acid Metabolites: Detection of Prospective Uremic Toxins in Serum by Multiple Reaction Monitoring Tandem Mass Spectrometry

            Alessandra F. Perna, Francesca Pane, Nunzio Sepe, Carolina Fontanarosa, Gabriella Pinto, Miriam Zacchia, Francesco Trepiccione, Evgeniya Anishchenko, Diego Ingrosso, Piero Pucci, and Angela Amoresano

 

3. Analysis of Vascular Hydrogen Sulfide Biosynthesis

            Thomas J. Lechuga and Dong-bao Chen

 

4. Measurement of Protein Persulfidation: Improved Tag-Switch Method

            Emilia Kouroussis, Bikash Adhikari, Jasmina Zivanovic, and Milos R. Filipovic

 

5. ProPerDP: A Protein Persulfide Detection Protocol

            Éva Dóka, Elias S.J. Arnér, Edward E. Schmidt, and Péter Nagy

 

6. Vascular Effects of H₂S-Donors: Fluorimetric Detection of H₂S Generation and Ion Channel Activation in Human Aortic Smooth Muscle Cells

            Alma Martelli, Valentina Citi, and Vincenzo Calderone

 

7. In Vitro Measurement of H₂S-Mediated Vasoactive Responses

            Sona Cacanyiova and Andrea Berenyiova

 

8. In Vivo Measurement of H₂S, Polysulfides, and ‘SSNO-mix’-Mediated Vasoactive Responses and Evaluation of 10 Hemodynamic Parameters from Rat Arterial Pulse Waveform

            Frantisek Kristek, Marian Grman, and Karol Ondrias

 

9. Simultaneous Measurements of Tension and Free H₂S in Mesenteric Arteries

            Elise R. Hedegaard, Anna K. Winther, and Ulf Simonsen

 

10. The Relaxant Mechanisms of Hydrogen Sulfide in Corpus Cavernosum

            Fatma Aydinoglu and Nuran Ogulener

 

11. Pharmacological Tools for the Study of H₂S Contribution to Angiogenesis

            Lucia Morbidelli, Martina Monti, and Erika Terzuoli

 

12. Central Administration of H₂S Donors for Studying Cardiovascular Effects of H₂S in Rats

            Marcin Ufnal and Artur Nowinski

 

13. Colonic Delivery of H₂S Donors for Studying Cardiovascular Effects of H₂S in Rats

            Marcin Ufnal and Tomasz Huc

 

14. Measurements for Sulfide-Mediated Inhibition of Myeloperoxidase Activity

            Dorottya Garai, Zoltán Pálinkás, József Balla, Anthony J. Kettle, and Péter Nagy

 

15. Vascular Myography to Examine Functional Responses of Isolated Blood Vessels

            Joanne Hart

Blurb

This book explores techniques for exploring hydrogen sulfide (H₂S) and its effects on the vascular system through numerous experimental animal models and vascular preparations. Alterations of vascular H₂S generation/signaling may be involved in the pathogenesis of systemic and pulmonary arterial hypertension, ischemic heart disease, ischemic stroke, preeclampsia, and erectile dysfunction, and H₂S also serves as an attractive target for pharmacotherapy of cardiovascular diseases, as well as possible effects on cancer, wound healing, and diabetic retinopathy, among other pathologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Vascular Effects of Hydrogen Sulfide: Methods and Protocols is an ideal aid for scientists working to extend our knowledge in this valuable and wide-ranging field of study.