Vaccinia Virus and Poxvirology: Methods and Protocols

ISBN-13: 9781617798757 / Angielski / Twarda / 2012 / 332 str.

Stuart N. Isaacs

Opis

Since the first edition of Vaccinia Virus and Poxvirology: Methods and Protocols was published, a number of important events related to poxvirology have occurred, such as FDA approval of a culture-based live smallpox vaccine and the vaccination of large numbers of U.S. military and relatively large numbers of U.S. civilians. Novel anti-poxvirus therapeutics have been developed and have been used in emergency settings. The second edition of Vaccinia Virus and Poxvirology expands upon the previous edition with entirely new sets of protocols. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Vaccinia Virus and Poxvirology: Methods and Protocols, Second Edition seeks to aid scientists in continuing to study poxviruses using new tools and approaches.

Spis treści

1. Working Safely with Vaccinia Virus: Laboratory Technique and Review of Published Cases of Accidental Laboratory Infections

Stuart N. Isaacs

 2. In-Fusion^® Cloning with Vaccinia Virus DNA Polymerase

Chad R. Irwin, Andrew Farmer, David O. Willer, and David H. Evans

 3. Genetic Manipulation of Poxviruses using Bacterial Artificial Chromosome Recombineering

Matthew G. Cottingham

 4. Easy and Efficient Protocols for Working with Recombinant Vaccinia Virus MVA

Melanie Kremer, Asisa Volz, Joost Kreijtz, Michael H Lehmann, and Gerd Sutter

 5. Isolation of Recombinant MVA using F13L Selection

Juana M. Sánchez-Puig, María M. Lorenzo, and Rafael Blasco

 6. Screening for Vaccinia Virus Egress Inhibitors - Separation of IMV, IEV, and EEV

Chelsea M. Byrd and Dennis E. Hruby

 7. Imaging of Vaccinia Virus Entry into HeLa Cells

Cheng-Yen Huang and Wen Chang

 8. New Method for the Assessment of Molluscum Contagiosum Virus Infectivity

Subuhi Sherwani, Niamh Blythe, Laura Farleigh and Joachim J Bugert

 9. An Intradermal Model for Vaccinia Virus Pathogenesis in Mice

Leon C. W. Lin, Stewart A. Smith and David C. Tscharke

 10. Measurements of Vaccinia Virus Dissemination using Whole Body Imaging: Approaches for Predicting of Lethality in Challenge Models and Testing of Vaccines and Anti-viral Treatments

Marina Zaitseva, Senta Kapnick, and Hana Golding

 11. Mousepox, a Small Animal Model of Smallpox

David Esteban, Scott Parker, Jill Schriewer, Hollyce Hartzler and R. Mark Buller

 12. Analyzing CD8 T Cells in Mouse Models of Poxvirus Infection

Inge EA Flesch, Yik Chun Wong and David C. Tscharke

 13. Generation and Characterization of Monoclonal Antibodies Specific for Vaccinia Virus

Xiangzhi Meng and Yan Xiang

 14. Bioinformatics for Analysis of Poxvirus Genomes

Melissa Da Silva and Chris Upton 

15. Antigen Presentation Assays to Investigate Uncharacterized Immunoregulatory Genes

Rachel L. Roper

16. Characterization of Poxvirus Encoded Proteins that Regulate Innate Immune Signaling Pathways

Florentina Rus, Kayla Morlock, Neal Silverman, Ngoc Pham, Girish J. Kotwal and William L. Marshall

17. Application of Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D)

18. Central Nervous System Distribution of the Poxviral Proteins After Intranasal Administration of Proteins and Titering of Vaccinia Virus in the Brain after Intracranial Administration

Amod P. Kulkarni, Dhirendra Govender, Lauriston A. Kellaway, and Girish J. Kotwal

Blurb

Since the first edition of Vaccinia Virus and Poxvirology: Methods and Protocols was published, a number of important events related to poxvirology have occurred, such as FDA approval of a culture-based live smallpox vaccine and the vaccination of large numbers of U.S. military personnel and relatively large numbers of U.S. civilians. Novel anti-poxvirus therapeutics have been developed and have been used in emergency settings. The second edition of Vaccinia Virus and Poxvirology expands upon the previous edition with entirely new sets of protocols. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 Authoritative and practical, Vaccinia Virus and Poxvirology: Methods and Protocols, Second Edition seeks to aid scientists in continuing to study poxviruses using new tools and approaches.