Vaccine Technologies for Veterinary Viral Diseases: Methods and Protocols

ISBN-13: 9781493930074 / Angielski / Twarda / 2015 / 265 str.

Alejandro Brun

Opis

This detailed volume explores the most popular antigen production and delivery strategies that have been tested in veterinary species. Viral vectors as well as genetic and protein subunit vaccines or large scale protein production systems are considered as well as an updated view of most options available for vaccine development, including the data obtained through experimental trials which contributes to the exploration and understanding of the immune mechanisms and immune correlates relevant in protection among different animal species. Written for the highly successful "Methods in Molecular Biology" series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, "Vaccine Technologies for Veterinary Viral Diseases: Methods and Protocols" facilitates access to well-established protocols to those beginning in this interesting and laborious field as well as providing important basic knowledge when attempting a novel vaccine design or platform.

Spis treści

1. Vaccines and Vaccination for Veterinary Viral Diseases: A General Overview

            Alejandro Brun

 

2. Using IC-Tagging Methodology for Production and Purification of Epitope-Loaded Protein Microspheres for Vaccination

            Natalia Barreiro-Piñeiro, Rebeca Menaya-Vargas, Alberto Brandariz-Núñez; Iria Otero-Romero, Irene Lostalé-Seijo, Javier Benavente, and José M. Martínez-Costas

 

3. Plant-Based Vaccine Antigen Production

            Hoang Trong Phan and Udo Conrad

 

4. DNA Vaccines: Experiences in the Swine Model

            Francesc Accensi, Fernando Rodríguez, and Paula L. Monteagudo

 

5. Novel Adjuvants and Immunomodulators for Veterinary Vaccines

            Peter M.H. Heegaard, Yongxiang Fang, and Gregers Jungersen

 

6. Polymerase Mechanism-Based Method of Viral Attenuation

            Cheri A. Lee, Avery August, Jamie J. Arnold, and Craig E. Cameron

 

7. BacMam Platform for Vaccine Antigen Delivery

            Günther M. Keil, Reiko Pollin, Claudia Müller, Katrin Giesow, and Horst Schirrmeier

 

8. Laboratory-Scale Production of Replication-Deficient Adenovirus Vectored Vaccines

            Susan J. Morris, Alison V. Turner, Nicola Green, and George M. Warimwe

 

9. Generation of Recombinant Modified Vaccinia Virus Ankara Encoding VP2, NS1, and VP7 Proteins of Bluetongue Virus

            Alejandro Marín-L

ópez and Javier Ortego

 

10. Generation of Recombinant Capripoxvirus Vectors for Vaccines and Gene Knockout Function Studies

            Hani Boshra, Jinxing Cao, and Shawn Babiuk

 

11. Recombinant Swinepox Virus for Veterinary Vaccine Development

            Hong-Jie Fan and Hui-Xing Lin

 

12. Generation and Selection of Orf Virus (ORFV) Recombinants

            Hanns-Joachim Rziha, Jörg Rohde, and Ralf Amann

 

13. Polycistronic Herpesvirus Amplicon Vectors for Veterinary Vaccine Development

            Anita Felicitas Meier, Andrea Sara Laimbacher, and Mathias Ackermann

 

14. Construction and Application of Newcastle Disease Virus-Based Vector Vaccines

            Paul J. Wichgers Schreur

 

15. Chimeric Pestivirus Experimental Vaccines

            Ilona Reimann, Sandra Blome, and Martin Beer

 

16. Analysis of the Cellular Immune Responses to Vaccines

            Nicholas Svitek, Evans L.N. Taracha, Rosemary Saya, Elias Awino, Vish Nene, and Lucilla Steinaa