Vaccine Adjuvants: Methods and Protocols

ISBN-13: 9781607615842 / Angielski / Twarda / 2010 / 314 str.

Gwyn Davies

Opis

'Vaccine Adjuvants' provides guidance on how to go about assessing the activity of adjuvant products. Rather than describing individual adjuvants, it includes detailed, practical information on measuring the responses produced by adjuvants in order to be relevant to the widest array of experiments.

Spis treści

1. Immunology of Vaccine Adjuvants Carla M.S. Ribeiro and Virgil E.J.C. Schijns 2. Preclinical Development of AS04 Nathalie Garçon 3. Nonclinical Safety Assessment of Vaccines and Adjuvants Jayanthi J. Wolf, Catherine V. Kaplanski, and Jose A. Lebron 4. Aluminum Adjuvants: Preparation, Application, Dosage, and Formulation with Antigen Erik B. Lindblad and Niels E. Schønberg 5. Freund's Complete and Incomplete Adjuvants, Preparation, and Quality Control Standards for Experimental Laboratory Animals Use Duncan E.S. Stewart-Tull 6. Liposomal Adjuvants: Preparation and Formulation with Antigens Jean Haensler 7. Micro/Nanoparticle Adjuvants: Preparation and Formulation with Antigens Padma Malyala and Manmohan Singh 8. Adjuvant Activity on Human Cells in vitro Dominique De Wit and Michel Goldman 9. Adjuvant Activity on Murine and Human Macrophages Valerie Quesniaux, Francois Erard, and Bernhard Ryffel 10. In vitro Effects of Adjuvants on B Cells Jörg Vollmer and Hanna Bellert 11. NKT Cell Responses to Glycolipid Activation Josianne Nitcheu Tefit, Gwyn Davies, and Vincent Serra 12. Tracking Dendritic Cells in vivo Catherine M. Rush and James M. Brewer 13. Adjuvant Effects on Antibody Titre Barry Walker and Ian Feavers 14. Functional Antibody Assays Ian Feavers and Barry Walker 15. Determining Adjuvant Activity on T Cell Function in vivo: Th Cells Thomas Lindenstrøm, Peter Andersen, and Else Marie Agger 16. Quantitative Multiparameter Assays to Measure the Effect of Adjuvants on Human Antigen Specific CD8 T Cell Responses Laurent Derré, Camilla Jandus, Petra Baumgaertner, Vilmos Posevitz,Estelle Devêvre, Pedro Romero, and Daniel E. Speiser 17. Large-Animal Model for Establishing E/T-Ratio of Adjuvants Luuk A. Th. Hilgers 18. Determining the Activity of Mucosal Adjuvants Barbara C. Baudner and Giuseppe Del Giudice 19. Adjuvant Activity of Cytokines Michael G. Tovey and Christophe Lallemand

Blurb

Spanning from discoveries in fundamental immunology to industrial and commercial concerns, the study of vaccine adjuvants has developed into an exciting area of work with great, vital potential in innovating techniques in which adjuvants may steer the immune system towards the responses required by unmet vaccination needs. In Vaccine Adjuvants: Methods and Protocols, expert researchers in the field provide clear and concise guidance on how to go about assessing the activity of adjuvant products. Rather than describing individual adjuvants, the volume strives to include detailed, practical information on measuring the responses produced by adjuvants in order to be relevant to the widest array of experiments. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and versatile, Vaccine Adjuvants: Methods and Protocols will enable those already pursuing vaccine adjuvant research, while also serving to stimulate discussion on how to best standardize adjuvant testing in order to facilitate meaningful comparisons, and above all, to aid in the prediction of which new products will most effectively and safely help to solve the current challenges in vaccination.