1. Analysis of Chromosomal Alterations in Urothelial Carcinoma Donatella Conconi and Angela Bentivegna
2. Analysis of Point Mutations in Clinical Samples of Urothelial Carcinoma Mustafa Alamyar and Ellen C. Zwarthoff
3. A Versatile Assay for Detection of Aberrant DNA Methylation in Bladder Cancer Stella Tommasi and Ahmad Besaratinia
4. Immunohistochemical Analysis of UC Tissues for Proliferation and Differentiation Markers Michael Rose and Nadine Therese Gaisa
5. Molecular Subtype Profiling of Urothelial Carcinoma Using a Subtype-Specific Immunohistochemistry Panel Gottfrid Sjödahl
PART II: Urothelial Carcinogenesis
6. Defining the Pathways of Urogenital Schistosomiasis-associated Urothelial Carcinogenesis through Transgenic and Bladder Wall Egg Injection Models Evaristus C. Mbanefo and Michael H. Hsieh
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7. Algorithm for the Automated Evaluation of NAT2 Genotypes Georg Michael, Ricarda Thier, Meinolf Blaszkewicz, Silvia Selinski, and Klaus Golka
8. Detection of APOBEC3 Proteins and Catalytic Activity in Urothelial Carcinoma Ananda Ayyappan Jaguva Vasudevan, Wolfgang Göring, Dieter Häussinger, and Carsten Münk
9. Oxidative Stress in Urothelial Carcinogenesis: Measurements of Protein Carbonylation and Intracellular Production of Reactive Oxygen Species Patcharawalai Whongsiri, Suchittra Phoyen, and Chanchai Boonla
PART III: Cellular and ANIMAL MODELS
10. Urothelial Carcinoma Stem Cells - current Concepts, Controversies, and Methods Jiri Hatina, Hamendra Singh Parmar, Michaela Kripnerova, Anastasia Hepburn, and Rakesh Heer
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12. Spheroid Cultures of Primary Urothelial Cancer Cells: Cancer Tissue-originated Spheroid (CTOS) Method Takahiro Yoshida, Hiroaki Okuyama, Hiroko Endo, and Masahiro lnoue
13. The N-butyl-N-4-hydroxybutyl Nitrosamine Mouse Urinary Bladder Cancer Model Paula Alexandra Oliveira, Cármen Vasconcelos-Nóbrega, Rui Manuel Gil da Costa, and Regina Arantes-Rodrigues
14. Patient-derived Bladder Cancer Xenografts Carina Bernardo and Lúcio Lara Santos
15.< Orthotopic Mouse Models of Urothelial Cancer Wolfgang Jäger, Igor Moskalev, Peter Raven, Akihiro Goriki, Samir Bidnur, and Peter C. Black
PART IV: Biomarkers
16. Quantification of microRNAs in Urine-derived Specimens Susanne Fuessel, Andrea Lohse-Fischer, Dana Vu Van, Karsten Salomo, Kati Erdmann, and Manfred P. Wirth
17. Quantitative RNA Analysis from Urine using Real Time PCR Lourdes Mengual and Mireia Olivan
18. DNA Methylation Analysis from Body Fluids Dimo Dietrich
19. Urinary Protein Markers for the Detection and Prognostication of Urothelial Carcinoma Tibor Szarvas, Péter Nyirády, Takashi Kobayashi, Osamu Ogawa, Hideki Furuya, and Charles J. Rosser
20. Isolation and Characterization of CTCs from Patients with Cancer of a Urothelial Origin Vladimir Bobek and Katarina Kolostova
PART V: Therapy Development
21. Epigenetic Treatment Options in Urothelial Carcinoma Guenter Niegisch, Maria Pinkerneil, and Michèle Hoffmann
22. Evaluation of Protein Levels of the Receptor Tyrosine Kinase ErbB3 in Serum Leandro S. D'Abronzo, Chong-Xian Pan, and Paramita M. Ghosh
23. Targeting the PI3K/AKT/mTOR Pathway in Bladder Cancer Anuja Sathe and Roman Nawroth
24. Visualization and Quantitative Measurement of Frug-induced Platinum Sdducts in the Nuclear DNA of Individual Cells by an Immuno-cytological Sssay Margarita Melnikova and Jürgen Thomale
This volume details standard techniques for the characterization of urothelial carcinoma as well as methods to investigate mechanisms of carcinogenesis. Chapters guide readers on cellular and animal models for urothelial carcinoma and related diseases, molecular analyses from body fluids, and new approaches to therapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Urothelial Carcinoma: Methods and Protocols hopes that the techniques described in this volume will contribute to the current upturn in research on urothelial carcinoma and to the application of its results in clinical practice.