Type 2 Diabetes: Methods and Protocols

ISBN-13: 9781493998807 / Angielski / Twarda / 2019 / 300 str.

Claire J. Stocker

Spis treści

Preface…

Table of Contents…

Contributing Authors…

1. Sixty Years of Drug Discovery for Type 2 Diabetes: Where Are We Now?

John C. Clapham

2. Practical Considerations for In Vivo Studies

Edward T. Wargent

3. Nutritional Models of Type 2 Diabetes Mellitus

Beverly Sara Műhlhäusler, Carla Toop, and Sheridan Gentili

4. Cheminformatics in the Identification of Drug Classes for the Treatment of Type 2 Diabetes

Paul W. Finn

5. Whole Exome Sequencing (WES) for Illumina HISeq using Solution-Based Capture

Milind C. Mahajan and Andrew S. McLellan

6. Gene Expression Mining in Type 2 Diabetes Research

Donald R. Dunbar

7. Pathways Enrichment Analysis of Gene Expression Data in Type 2 Diabetes

Maysson Ibrahim

8. Diagnostic Genetic Testing for Monogenic Diabetes and Congenital Hyperinsulinemia

Jayne A.L. Houghton

9. The Isolation and Purification of Rodent Pancreatic Islets of Langerhans

Jacqueline F. O’Dowd and Claire J. Stocker

10. Characterization of Islet Leukocyte Populations in Human and Murine Islets by Flow Cytometry

Matthew J. Butcher, Michelle B. Trevino, Yumi Imai, and Elena V. Galkina

11. Analysis of Histone Modifications in Rodent Pancreatic Islets by Native Chromatin Immunoprecipitation

Ionel Sandovici, Lisa Nicholas, and Laura P. O’Neill

12. Quantification of Pancreatic Islets: Using Image Analysis Tools

Parvathy E. Harikumar

13. The Measurement of Calcium Signaling In Beta Cell Lines using Epifluorescence and Confocal Microscopy

Joanne L. Selway

14. Nitric Oxide and Redox State Measurements in Pancreatic Beta Cells

Rodrigo Carlessi, Vinicius Cruzat, Younan Chen, and Philip Newsholme

15. Primary Adipocytes as a Model for Insulin Sensitivity

Mohamed S. Zaibi

16. Assessing Islet Transplantation Outcome in Mice

Aileen J.F. King and Chloe L. Rackham

17. The Measurement of Insulin Secretion using Pancreas Perfusion in the Rodent

Edward T. Wargent

Blurb

This second edition volume expands on the previous editions with updates on the commonly used techniques and analysis used by clinicians and scientists to study diabetes. The chapters in this book cover topics such as nutritional models of type 2 diabetes mellitus; the isolation and purification of rodent pancreatic islets of Langerhans; nitric oxide and redox state measurements in pancreatic beta cells; and primary adipocytes as a model for insulin sensitivity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Type 2 Diabetes: Methods and Protocols, Second Edition is a valuable resource for all researchers who are interested in learning more about this important and evolving field.