Tumor Suppressor Genes: Volume 2: Regulation, Function, and Medicinal Applications

ISBN-13: 9781489939142 / Angielski / Miękka / 2013 / 657 str.

Wafik S. El-Deiry

Opis

It has become clear that tumors result from excessive cell proliferation and a corresponding reduction in cell death caused by the successive accumulation of mutations in key regulatory target genes over time. During the 1980s, a number of oncogenes were characterized, whereas from the 1990s to the present, the emp- sis has shifted to tumor suppressor genes (TSGs). It has become clear that oncogenes and TSGs function in the same pathways, providing positive and negative growth regulatory activities. The signaling pathways controlled by these genes involve virtually every process in cell biology, including nuclear events, cell cycle, cell death, cytoskeletal, cell membrane, angiogenesis, and cell adhesion effects. Mu- tions in tumor suppressor genes have been identified in familial cancer syndromes, and the same genes in many cases have been found to be mutationally inactivated in sporadically occurring cancers. In their normal state, TSGs control cancer development and progression, as well as contribute to the sensitivity of cancers to a variety of therapeutics.Understanding the classes of TSGs, the biochemical pa- ways they function in, and how they are regulated provides an essential lesson in cancer biology. We cannot hope to advance our current knowledge and to develop new and more effective therapies without understanding the relevant pathways and how they influence the present approaches to therapy. Moreover, it is important to be able to access not only the powerful tools now available to discover these genes, but also their links to cell biology and growth control.

Recenzje

"The book will prove a great help to everybody who would like to enter the field of experimental and or clinical research. Those who are interested in basic understanding microbiology of cancer will enjoy this as well." -Neoplasma

Spis treści

Part I. Understanding the Function and Regulation of Tumor Suppressor Genes Utilizing NMR to Study the Structure of Growth-Inhibitory Proteins Francesca M. Marassi Generation and Application of Phospho-Specific Antibodies for p53 and pRB Yoichi Taya, Kiichiro Nakajima, Kumiko Yoshizawa-Kumagaye, and Katsuyuki Tamai Stability and Ubiquitination of the Tumor Suppressor Protein p53 Lesley J. Jardine and Carl G. Maki Role of Tumor Suppressors in DNA Damage Response Bin-Bing S. Zhou, Michael R. Mattern, and Kum Kum Khanna In Situ Hybridization in Cancer and Normal Tissue Shrihari Kadkol, Jeremy Juang, and Tzyy-choou Wu Genetic Strategies in Saccharomyces cerevisiae to Study Human Tumor Suppressor Genes Takahiko Kobayashi, Ting Wang, Hua Qian, and Rainer K. Brachmann Electrophoretic Mobility Shift Analysis of the DNA Binding of Tumor Suppressor Gene Products Edward C. Thornborrow, Matthew Maurer, and James J. Manfredi Analysis of Gene Promoter Regulation by Tumor Suppressor Genes Kumaravel Somasundaram, Sanjeev Das, Smita Lakhotia, and Narendra Wajapeyee DNA Footprinting M. Stacey Ricci and Wafik S. El-Deiry Identification of DNA-Binding of Tumor Suppressor Genes by Chromatin Immunoprecipitation Timothy K. MacLachlan and Wafik S. El-Deiry Co-immunoprecipitation of Tumor Suppressor Protein-Interacting Proteins Li-Kuo Su Microarray Approaches for Analysis of Tumor Suppressor Gene Function Sally A. Amundson and Albert J. Fornace, Jr. Analysis of Tumor Suppressor Gene-Induced Senescence Judith Campisi Yeast Two-Hybrid Screening as a Means of Deciphering Tumor Suppressor Pathways E. Robert McDonald III and Wafik S. El-Deiry Somatic Cell Knockouts of Tumor Suppressor Genes Ruth Hemmer, Wenyi Wei, Annie Dutriaux, and John M. Sedivy Colony Growth Suppression by Tumor Suppressor Genes Gen Sheng Wu Flow Cytometric Analysis of Cell Cycle Control by Tumor Suppressor Genes David T. Dicker and Wafik S. El-Deiry Analysis of Cyclin-Dependent Kinase Activity Timothy K. MacLachlan and Wafik S. El-Deiry Tumor Suppressor Gene-Inducible Cell Lines Michael Dohn, Susan Nozell, Amy Willis, and Xinbin Chen In Vitro Models of Early Neoplastic Transformation of Human Mammary Epithelial Cells Vimla Band Tumor Suppression Through Angiogenesis Inhibition Andrew M. Arsham and M. Celeste Simon Flow Cytometric Analysis of Tumor Suppressor Gene-Induced Apoptosis David T. Dicker and Wafik S. El-Deiry Functional Analysis of Tumor Suppressor Genes in Mice Stephen N. Jones and Lawrence A. Donehower The Tissue Microenvironment as an Epigenetic Tumor Modifier Meredith Unger and Valerie M. Weaver Analyzing the Function of Tumor Suppressor Genes Using a Drosophila Model Raymond A. Pagliarini, Ana T. Quiñones, and Tian Xu Assembling a Tumor Progression Model Mark Redston Conformation-Sensitive Gel Electrophoresis for Detecting BRCA1 Mutations Smita Lakhotia and Kumaravel Somasundaram Part II. Medicinal Applications of Tumor Suppressors Conversion Technology and Cancer Predispositions Nickolas Papadopoulos Discovering Novel Anticancer Drugs: Practical Aspects and Recent Advances Cristina T. Lewis and Patrick M. O'Connor Targets in Apoptosis Signaling: Promise of Selective Anticancer Therapy Ramadevi Nimmanapalli and Kapil Bhalla Tumor Deprivation of Oxygen and Tumor Suppressor Gene Function Zhong Yun and Amato J. Giaccia Hormonal and Differentiation Agents in Cancer Growth Suppression Mikhail V. Blagosklonny Regulation of NF-kB by Oncoproteins and

O autorze

Wafik S. El-Deiry, M.D., Ph.D, is Professor of Medicine, Chief, Hematology/Oncology, Rose Dunlap Division Chair in Hematology/Oncology, and Associate Director for Translational Researchat Penn State Hershey Cancer Institute. Dr. El-Deiry is a well-respected and notable figure among cancer researchers, scientists, and clinicians. He has published almost 300 papers in his career and has over 29,000 totol citations, with an excellent h-index of 70.

Blurb

Powerful new tools are now available to discover and understand tumor suppressor genes (TSGs) and the biochemical mechanisms by which they control cancer development and progression. In Tumor Suppressor Genes, Volume 2: Regulation, Function, and Medicinal Applications, leading physician scientists and researchers explore the cell biology and biochemical function of the tumor suppressor genes, as well as their physiological role in vivo. The authors detail the physical methods-NMR, microarray approaches, posttranslational structure analysis, analysis of regulation at the gene expression and protein signaling levels-used to understand the function of tumor suppressor genes. In vivo approaches discussed include studies in yeast, Drosophila, mice, and human tumors. The authors also discuss how one ultimately derives a "tumor progression model," the drug discovery process, and the weaknesses of cancers that allow drugs to selectively kill them. Other aspects reviewed include screening strategies, targeting the blood supply of tumors, and the replacement of defective cancer genes to achieve a therapeutic effect. The companion volume of this set, Tumor Suppressor Genes, Volume 1: Pathways and Isolation Strategies, covers all known tumor suppressor genes, describing their cellular signaling pathways, how they were discovered, how they can be analyzed, and how they can be optimally used in cancer therapeutics
Comprehensive and authoritative, the two volumes of Tumor Suppressor Genes provide an unparalleled compilation of key data on all known tumor suppressor pathways and a treasury of techniques for their discovery, analysis, and uses in cancer therapeutics.