Transplantation Immunology: Methods and Protocols

ISBN-13: 9781627034920 / Angielski / Twarda / 2013 / 411 str.

Andrea A. Zachary; Mary S. Leffell

Opis

After decades of research in clinical transplantation, new techniques have been developed that permit a further understanding of the immune mechanisms underlying immune recognition of allografts and a more accurate and thorough evaluation of compatibility between donors and recipients. The second edition of Transplantation Immunology: Methods and Protocols expands upon the previous edition with current, detailed methods in transplantation immunology. The new methods chapters cover four major areas that are being applied in compatibility evaluations and ongoing transplantation immunology research. Seven overview chapters provide reviews of the molecular basis for alloreactivity, current understanding of humoral and cellular mechanisms, as well as new developments in thoracic organ transplantation, composite tissue transplantation and in the transplantation of sensitized patients. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Transplantation Immunology: Methods and Protocol, Second Edition is devoted to transplantation immunology, both in the practice of compatibility testing and in transplantation research.

Recenzje

From the reviews of the second edition:

"The purpose is to provide an overview of key areas of research in transplant immunology and detailed descriptions of relevant methodology. An appropriate audience includes solid organ transplant physicians, immunologists, and immunology laboratory staff. ... I find this book very useful. It covers important topics in transplant immunology in great detail, and is a wonderful opportunity to review the current understanding of important topics by leaders in the field." (Christina Klein, Doody's Book Reviews, November, 2013)

Spis treści

PART I: Overview Chapters1. Alloreactivity

Sidonia B.G. Eckle, Jamie Rossjohn, and James McCluskey

 2. Antibodies in Transplantation: The Effects of HLA and Non-HLA Antibody
Binding and Mechanisms of Injury

Nicole M. Valenzuela and Elaine F. Reed

 3. Cell Mediated Rejection

Kathryn J Wood, Masaaki Zaitsu and Ryoichi Goto

 4. Transplantation Tolerance

Colin Brinkman, Bryna Burrell, Joseph Scalea, and Jonathan Bromberg

 5. Composite Tissue Transplantation

Gerald Brandacher

 6. Transplantation of the Sensitized Patient: Histocompatibility Testing

Robert A. Montgomery, Mary S. Leffell, and Andrea A. Zachary

 7. Thoracic Organ Transplantation: Laboratory Methods

Jignesh K. Patel and Jon A. Kobashigawa

  

PART II: Methods Chapters

 8. HLA Typing by Sequence Specific Primers

Mike Bunce, Biofortuna and Ben Passey, Biofortuna

 9. Human Leukocyte Antigen (HLA) Typing By DNA Sequencing

Ana Lazaro, Bin Tu, Ruyan Yang, Yi Xiao, Kanthi Kariyawasam, Jennifer Ng, Carolyn and Katovich Hurley

 10. Next-Generation HLA Sequencing using the 454 GS FLX System

Elizabeth A. Trachtenberg and Cherie L. Holcomb

 11. Chimerism Testing by Quantitative PCR  Using Indel Markers

Ketevan Gendzekhadze, Laima Gaidulis, and David Senitzer

 12. Gene-specific PCR Typing of Killer Cell Immunoglobulin-like Receptors (KIR)

Raja Rajalingam and Elham Ashouri

 13. Complement-dependent Cytotoxicity Crossmatch

Jeremy Ryan Peña, Donna Fitzpatrick, and Susan L. Saidman

 14. Lymphocyte Crossmatching by Flow Cytometry

Robert A. Bray

 15. Solid Phase Assay Measuring C4d Deposition to Determine Complement Fixation by HLA Specific Antibodies

John D Smith and  Marlene L Rose

 16. C1q Assay for the Detection of Complement Fixing Antibody to HLA Antigens

Ge Chen and Dolly B. Tyan

 17. Tetramer Staining for the Detection of HLA-specific B cells

Donna P. Lucas, Mary S. Leffell, and Andrea A. Zachary

 18. A Flow Cytometric Crossmatch Test Using Endothelial Precursor Cells Isolated from Peripheral Blood

Annette M. Jackson, Donna P. Lucas, Jessica L. Badders

 19. The Detection of Antibodies to the Angiotensin II-Type 1 Receptor in Transplantation

Duska Dragun

 20. Detection of Antibodies to Self-antigens (K-alpha 1 Tubulin, Collagen I, II, IV and V Myosin and Vimentin) by Enzyme-Linked Immunosorbent Assay (ELISA)

Nayan J. Sarma, Venkataswarup Tiriveedhi, and T. Mohanakumar

 21. Cylex  ImmuKnow Cell Function Assay

Adriana Zeevi and John Lunz

 22. Detection of Intracellular Cytokines

Nancy L. Reinsmoen and Chih-Hung Lai

 23. Tolerogenic Dendritic Cells and Induction of T Suppresson Cells in Transplant Recipients

George Vlad and Nicole Suciu-Foca

 24. Discovery and Customized Validation of Antibody Targets by Protein Arrays and Indirect ELISA

Tara K. Sigdel, and Minnie. M. Sarwal

 25. RNA Purification and Expression Analysis using Microarrays and RNA Deep Sequencing

Steven R. Head, Tony Mondala, Terri Gelbart, and Phillip Ordoukhanian