Transplantation Immunology: Methods and Protocols

ISBN-13: 9781617376320 / Angielski / Miękka / 2010 / 430 str.

Philip Hornick

Opis

Leading clinicians and scientists in solid organ transplantation review the current status of the field and describe cutting-edge techniques for detecting the immune response to the allografted organ. The authors present the latest techniques for HLA typing, detecting HLA antibodies, and monitoring T-cell response, and examine more specialized methods utilizing proteomics, laser dissection microscopy, and real-time polymerase chain reaction. The area of tolerance induction and reprogramming of the immune system is also covered, along with a discussion of up-to-date methods of organ preservation, of today's optimal immunosuppressive drug regimens, as well as the difficulty of mimicking chronic rejection in experimental models. Introductory chapters provide a theoretical update on current practices in renal, liver, islet, and lung transplantation and on the pathways of antigen presentation and chronic rejection.

Spis treści

Current Status of Renal Transplantation Christopher J. Callaghan and J. Andrew Bradley Current Status of Liver Transplantation Peter J. Friend and Charles J. Imber Current Status of Clinical Islet Cell Transplantation Jonathan R. T. Lakey, Mohammadreza Mirbolooki, and A. M. James Shapiro Current Status of Lung Transplantation Allan R. Glanville Chronic Rejection in the Heart Philip Hornick and Marlene Rose Direct and Indirect Allorecognition Philip Hornick HLA Typing and Its Influence on Organ Transplantation Stephen Sheldon and Kay Poulton Strategies for Gene Transfer to Solid Organs: Viral Vectors Charlotte Lawson Nonviral Vectors Louise Collins Detection and Clinical Relevance of Antibodies After Transplantation John D. Smith and Marlene Rose Reprogramming the Immune System Using Antibodies Luis Graca and Herman Waldmann In Vitro Assays for Immune Monitoring in Transplantation Maria P. Hernandez-Fuentes and Alan Salama Proteomics and Laser Microdissection Emma McGregor and Ayesha De Souza Real-Time Quantitative Polymerase Chain Reaction in Cardiac Transplant Research Leanne E. Felkin, Anne B. Taegtmeyer, and Paul J. R. Barton Organ Preservation Mark Hicks, Alfred Hing, Ling Gao, Jonathon Ryan, and Peter S. MacDonald Pharmacological Manipulation of the Rejection Response Peter Mark Anthony Hopkins Experimental Models of Graft Arteriosclerosis Bezhad Soleimani and Victor C. Shi Index

Blurb

Technical innovations in the laboratory over the past ten years have greatly improved our understanding of the immunological mechanisms of transplanted organ rejection. In Transplantation Immunology: Methods and Protocols, leading experts in solid organ transplantation review the current status of the field and describe cutting-edge techniques for detecting the immune response to the allografted organ. The authors present the latest techniques for HLA typing, detecting HLA antibodies, and monitoring T-cell response, and examine more specialized methods of utilizing proteomics, laser dissection microscopy, and real-time polymerase chain reaction. The areas of tolerance induction and reprogramming of the immune system are also covered, along with a discussion of up-to-date methods of organ preservation, of today's optimal immunosuppressive drug regimens, as well as the difficulty of mimicking chronic rejection in experimental models. Introductory chapters provide a theoretical update on current practices in renal, liver, islet, and lung transplantation and on the pathways of antigen presentation and chronic rejection.
State of the art and highly practical, Transplantation Immunology: Methods and Protocols illuminates for clinicians and scientists-both newcomers and experts-the new world of detecting and monitoring patients' immunological responses to solid organ transplantation.