Transgenic Maize: Methods and Protocols

ISBN-13: 9781617379352 / Angielski / Miękka / 2010 / 188 str.

M. Paul Scott

Opis

As increasing global population and continuing economic development ensure the need for further production and cultivation of maize, the necessity of the application of transgenic technology to this model species and crop plant grows steadily. In Transgenic Maize: Methods and Protocols, experts in the various disciplines of the field contribute readily reproducible protocols covering such topics as transformation methods, transgenic maize in research, the analysis of transgenic plants, and breeding with transgenes. Written in the highly successful Methods in Molecular Biology(TM) series format, the chapters present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Cutting-edge and easy to use, Transgenic Maize: Methods and Protocols will greatly aid researchers wishing to use these procedures in their own laboratories, and will provide readers with a better understanding of the experiments being performed by cooperators or fee-for-service labs.

Recenzje

From the reviews:

"This book is number 526 in the popular 'Methods in Molecular Biology' series, which is renowned for its targeted protocol chapters, each with photographs, recipes and a set of notes for additional practical guidance and to avoid common pitfalls. ... There is a lot to like about this collection of practical chapters, which represent an excellent investment for the applied maize researcher, who could be a student doing an undergraduate project through to a professional scientist in a dedicated transformation laboratory." (Huw D. Jones, Annals of Botany, Vol. 109 (7), June, 2012)

Spis treści

Part I: Introduction 1. Design of Gene Constructs for Transgenic Maize Dong Liu Part II: Transformation Methods 2. Transient Expression of GFP in Immature Seed Tissues Colin T. Shepherd 3. Biolistic Gun Mediated Maize Genetic Transformation Kan Wang and Bronwyn Frame 4. Agrobacterium-Mediated Maize Transformation: Immature Embryos Versus Callus Vladimir Sidorov and David Duncan 5. Whiskers-Mediated Maize Transformation Joseph F. Petolino and Nicole L. Arnold Part III: Transgenic Maize in Research 6. Methods for Generation and Analysis of Fluorescent Protein Tagged Maize Lines Amitabh Mohanty, Yan Yang, Anding Luo, Anne Sylvester, and David Jackson 7. Use of Transgene-Induced RNAi to Regulate Endogenous Gene Expression Karen M. McGinnis 8. Plasmid Rescue: Recovery of Flanking Genomic Sequences from Transgenic Transposon Insertion Sites Guo-Ling Nan and Virginia Walbot Part IV: Analysis of Transgenic Plants 9. Non-Radioactive Genomic DNA Blots for Detection of Low Abundant Sequences in Transgenic Maize Guo-Ling Nan and Virginia Walbot 10. Tissue-Print Immuno-Detection of Transgene Products in Endosperm for High-Throughput Screening of Seeds M. Paul Scott 11. Determination of Transgene Copy Number by Real-Time Quantitative-PCR Colin T. Shepherd, Adrienne N. Moran Lauter, and M. Paul Scott Part V: Breeding with Transgenes 12. Herbicide Resistance Screening Assay Joan M. Peterson 13. Characterizing Transgene Inheritance Earl H. Bicar 14. Backcross Breeding Karla E. Vogel Appendix A: Sample APHIS Notification of Release of Regulated Material Appendix B: Sample SOP for Field Release

Blurb

As increasing global population and continuing economic development ensure the need for further production and cultivation of maize, the necessity of the application of transgenic technology to this model species and crop plant grows steadily. In Transgenic Maize: Methods and Protocols, experts in the various disciplines of the field contribute readily reproducible protocols covering such topics as transformation methods, transgenic maize in research, the analysis of transgenic plants, and breeding with transgenes.  Written in the highly successful Methods in Molecular Biology™ series format, the chapters present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Cutting-edge and easy to use, Transgenic Maize: Methods and Protocols will greatly aid researchers wishing to use these procedures in their own laboratories, and will provide readers with a better understanding of the experiments being performed by cooperators or fee-for-service labs.