Transgenesis Techniques: Principles and Protocols

ISBN-13: 9781603270182 / Angielski / Twarda / 2009 / 335 str.

Elizabeth J. Cartwright

Opis

One of the major challenges currently facing the scientific community is to understand the function of the multitude of protein-coding genes that were revealed when the human genome was fully sequenced. In Transgenesis Techniques: Principles and Protocols, Third Edition, experts in the field fully update and expand upon the previous edition in order to detail the transgenic techniques currently used to modify the genome. The volume explores classic procedures to genetically modify mice and other model organisms, as well as cutting-edge practices involving microinjection, site-specific recombination systems, cryopreservation, and many other topics. Written in the highly successful Methods in Molecular Biology(TM) series format, the chapters include brief introductions to their subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Transgenesis Techniques: Principles and Protocols, Third Edition is a comprehensive practical guide to the generation of transgenic animals and an invaluable source of information in any lab currently involved in transgenic techniques.

Spis treści

Part I: Transgenesis in Various Model Systems 1. Transgenesis in Drosophila melanogaster Leonie Ringrose 2. Transgenesis in Caenorhabditis elegans Matthias Rieckher, Nikos Kourtis, Angela Pasparaki, and Nektarios Tavernarakis 3. Transgenesis in Zebrafish with the Tol2 Transposon System Maximiliano L. Suster, Hiroshi Kikuta, Akihiro Urasaki, Kazuhide Asakawa, and Koichi Kawakami 4. Generation of Transgenic Frogs Jana Loeber, Fong Cheng Pan, and Tomas Pieler 5. Pronuclear DNA Injection for the Production of Transgenic Rats Jean Cozzi, Ignacio Anegon, Valérie Braun, Anne-Catherine Gross, Christel Merrouche, and Yacine Cherifi Part II: Transgenesis in the Mouse 6. Cell-Type Specific Transgenesis in the Mouse James Gulick and Jeffrey Robbins 7. Transgene Design and Delivery into the Mouse Genome: Keys to Success Lydia Teboul 8. Overexpression Transgenesis in Mouse: Pronuclear Injection Wendy J.K. Gardiner and Lydia Teboul 9. Gene Targeting Vectors J. Simon C. Arthur and Victoria A. McGuire 10. Gene Trap: Knockout on the Fast Lane Melanie Ullrich and Kai Schuh 11. Culture of Murine Embryonic Stem Cells Ivana Barbaric and T. Neil Dear 12. Targeting Embryonic Stem Cells Roland H. Friedel 13. Generation of Chimeras by Microinjection Anne Plück and Christian Klasen 14. Generation of Chimeras by Morula Aggregation Anne Plück and Christian Klasen 15. Surgical Techniques for the Generation of Mutant Mice Anne Plück and Christian Klasen 16. Site-specific Recombinases for Manipulation of the Mouse Genome Marie-ChristineBirling, Françoise Gofflot, and Xavier Warot 17. Cre Transgenic Mouse Lines Xin Wang 18. Large-Scale Mouse Mutagenesis Elizabeth J. Cartwright 19. Dedicated Mouse Production and Husbandry Lucie Vizor and Sara Wells 20. Biological Methods for Archiving and Maintaining Mutant Laboratory Mice: Part I - Conserving Mutant Strains Martin D. Fray 21. Biological Methods for Archiving and Maintaining Mutant Laboratory Mice: Part II - Recovery and Distribution of Conserved Mutant Strains Martin D. Fray

Blurb

One of the major challenges currently facing the scientific community is to understand the function of the multitude of protein-coding genes that were revealed when the human genome was fully sequenced. In Transgenesis Techniques: Principles and Protocols, Third Edition, experts in the field fully update and expand upon the previous edition in order to detail the transgenic techniques currently used to modify the genome.  The volume explores classic procedures to genetically modify mice and other model organisms, as well as cutting-edge practices involving microinjection, site-specific recombination systems, cryopreservation, and many other topics. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include brief introductions to their subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.

Comprehensive and state-of-the-art, Transgenesis Techniques: Principles and Protocols, Third Edition is a comprehensive practical guide to the generation of transgenic animals and an invaluable source of information in any lab currently involved in transgenic techniques.