Trafficking Inside Cells: Pathways, Mechanisms and Regulation

ISBN-13: 9781461424659 / Angielski / Miękka / 2012 / 445 str.

Nava Segev

Opis

The SecretoryPathway The transport ofproteins and lipids from their site ofsynthesis at the endoplasmic reticu lum (ER) to the cellsurface ismediated by the secretory pathway and isan essential process in eukaryotic organisms. A great variety ofmolecules are extruded from cellsby the action ofthe secretory pathway, including extracellular matrix components that provide the foundation for constructing tissuesand organs. Moreover, this pathway playsa major role in the biogenesisof the plasma membraneand itsexpansion before celldivision. Therefore, withoutsecretion there would be no cells, tissuesor organs, and so it issafeto saythat we oweourvery existence to the secretory pathway. To understand the process ofsecretion we must learn about the organelles that compose the secretory pathway; the ER and Golgi apparatus, and the transport vesicles these or ganelles produce. The membrane ofthese organelles is primarily synthesized and assembled at the ER but with contributions from mitochondria (phosphatidylethanolamine) and the Golgi apparatus (sphingolipids). Newly synthesized proteins destined for secretion gain en try into the secretory pathway by translocation across the ER membrane. This translocation apparatus also integrates proteins into the membrane and establishes their topology with respect to the lipid bilayer (seeChapter 7). Many secretory proteins are covalently modified with oligosaccharides to produce glycoproteins, a biosynthetic process initiated in the ER and continued in the Golgi apparatus. Once proteins are properly folded and modified in the ER, they are allowed to leave and are ushered into COPlI-coated carrier vesiclesforming at specific exit sites (see Chapters 1 and 8).

Spis treści

Section I. Compartments and Pathways 1. Overview of Intracellular Compartments and Trafficking Pathways Andrei A. Tokarev, Aixa Alfonso and Nava Segev 2. How We Study Protein Transport Mary L. Preuss, Peggy Weidman and Erik Nielsen 3. The Golgi Apparatus Zhaolin Hua and Todd R. Graham 4. The Endocytic Pathway Elizabeth Conibear and Yuen Yi C. Tam 5. Regulated Secretion Naveen Nagarajan, Kenneth L. Custer and Sandra Bajjalieh Section II. Mechanisms 6. Overview of Protein Trafficking Mechanisms Giancarlo Costaguta and Gregory S. Payne 7. Entry into the Endoplasmic Reticulum: Protein Translocation, Folding and Quality Control Sheara W. Fewell and Jeffrey L. Brodsky 8. COP-Mediated Vesicle Transport Silvere Pagant and Elizabeth Miller 9. Clathrin-Mediated Endocytosis Peter S. McPherson, Brigitte Ritter and Beverly Wendland 10. Biogenesis of Dense-Core Secretory Granules Grant R. Bowman, Andrew T. Cowan and Aaron P. Turkewitz 11. Lipid-Dependent Membrane Remodelling in Protein Trafficking Priya P. Chandra and Nicholas T. Ktistakis 12. Carrier Motility Marcin J. Wozniak and Victoria J. Allan 13. Tethering Factors Vladimir Lupashin and Elizabeth Sztul 14. Intracellular Membrane Fusion Dalu Xu and Jesse C. Hay Section III. Regulation and Coordination with Other Cellular Processes 15. Regulation and Coordination of Intracellular Trafficking: An Overview Julie Donaldson and Nava Segev 16. Regulation of Protein Trafficking by GTP-Binding Proteins Michel Franco, Philippe Chavrier and Florence Niedergang 17. Posttranslational Control of Protein Trafficking in the Post-Golgi Secretory and Endocytic Pathway &nb