Tissue-Resident Macrophages

ISBN-13: 9781071634363 / Angielski / Twarda / 2023

Opis

This detailed book delves into the diverse techniques and applications to target, isolate, image, phenotype, and analyze tissue-resident and monocyte-derived macrophages. The contents aim to describe the current knowledge about macrophage development and function which forces the scientific field to move beyond the previously described M1/M2 macrophage paradigm to be able to dissect macrophage functions within their specific niches during health and disease. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Thorough and practical, Tissue-Resident Macrophages: Methods and Protocols provides scientists entering the macrophage field with information and tools that allow them to dive into the state-of-the-art methodology used in this vital field.

Spis treści

1. Macrophage Development and Function

            Nikola Makdissi

 

2. Fate Mapping Macrophages: From Ontogeny to Functions

            Anna Ahlback and Rebecca Gentek

 

3. Studying Autophagy in Microglia: Overcoming the Obstacles

            Ainhoa Plaza-Zabala and Amanda Sierra

 

4. Hemocyte Nuclei Isolation from Adult Drosophila melanogaster for snRNA-Seq

            Fabian Hersperger, Melanie Kastl, Katrin Paeschke, and Katrin Kierdorf

 

5. Isolation of Tissue Macrophages in Adult Zebrafish

            Mireia Rovira, Jennifer Pozo, Magali Miserocchi, and Valérie Wittamer

 

6. Genetic and Immunohistochemistry Tools to Visualize Rat Macrophages In Situ

            Stephen Huang, Dylan Carter-Cusack, Emma Maxwell, Omkar L. Patkar, Katharine M. Irvine, and David A. Hume

 

7. Phenotyping of Macrophages in Human Immune System Mice

            Leonie Voss, Carmen Reitinger, and Anja Lux

 

8. Fate-Mapping of Yolk Sac-Derived Macrophages

            Iva Splichalova and Elvira Mass

 

9. Fate-Mapping of Hematopoietic Stem Cell-Derived Macrophages

            Katharina Mauel and Elvira Mass

 

10. Isolation and Flow Cytometry Analysis of Macrophages from White Adipose Tissue

            Dailia Ribeiro, Seniz Yüksel, Andreas Dolf, and Dagmar Wachten

 

11. Isolation and Flow Cytometry Analysis of Macrophages from the Dermis

            Aaron James Forde and Julia Kolter

 

12. Isolation and Flow Cytometry Analysis of Macrophages from the Kidney

            Sarah J. Bland, Alex Yashchenko, and Kurt A. Zimmerman

 

13. Isolation and Flow Cytometry Analysis of Intestinal Macrophages

            Maria Francesca Viola and Guy Boeckxstaens

 

14. Isolation and Characterization of Testis Macrophages Using Flow Cytometry

            Myriam Sekias, Myriam Baratin, and Marc Bajenoff

 

15. Studying Macrophages in the Murine Fatty Liver Using Flow Cytometry and Confocal Microscopy

            Zhuangzhuang Liu, Pieter A. Louwe, and Charlotte L. Scott

 

16. Isolation, Ex Vivo Expansion, and Lentiviral Transduction of Alveolar Macrophages

            Clara Jana-Lui Busch, Sethuraman Subramanian, Javier Linares, Jérémy Favret, Ridzky Anis Advent Yuda, and Michael H. Sieweke

 

17. Translatome Profiling of Tissue-Resident Macrophages Using the RiboTag Approach

            Jung-Seok Kim, Zhana Haimon, Sigalit Boura-Halfon, and Steffen Jung

 

18. Spectral Flow Cytometry Analysis of Resident Tissue Macrophages

            Wan Ting Kong, Mathilde Bied, and Florent Ginhoux

 

19. Unveiling Macrophage Heterogeneity and Their Spatial Distribution Using Multiplexed Tissue Imaging

            David Alejandro Bejarano and Andreas Schlitzer

 

20. 3D Imaging of Macrophages in Complete Organs

            Carole Siret and Serge A. van de Pavert

 

21. Whole-Mount Imaging of Adipose Tissue Macrophages

            Lydia Sorokin and Luis Henrique Corrêa

 

22. Functional In Vivo Imaging of Macrophages

            Anja Wegner, Ralph Palmisano, and Stefan Uderhardt

 

23. Elucidating Immune Monitoring of Tissue-Resident Macrophages by Intravital Microscopy

            Karolin W. Hublitz and Efstathios G. Stamatiades

 

24. Combined Host-Pathogen Fate Mapping to Investigate Lung Macrophages in Viral Infection

            Sebastian Baasch, Julia Henschel, and Philipp Henneke

 

25. Measuring the Metabolic State of Tissue-Resident Macrophages via SCENITH

            Andrea Vogel, Paulina Garcia Gonzalez, and Rafael J. Argüello

 

26. Analyzing Fcg-Receptor Interactions on Monocytes with the Proximity Ligation Assay (PLA)

            Sibel Kara and Falk Nimmerjahn

 

27. Studying Efferocytosis Dynamics in Tissue-Resident Macrophages Ex Vivo

            Irene Aranda-Pardos, Achmet Imam-Chasan, and Noelia Alonso-Gonzalez

 

28. Monitoring of Inflammasome Activation of Macrophages and Microglia In Vitro, Part 1: Cell Preparation and Inflammasome Stimulation

            Marta Lovotti, Matthew S.J. Mangan, Róisín M. McManus, Kateryna Shkarina, Matilde B. Vasconcelos, and Eicke Latz

 

29. Monitoring of Inflammasome Activation of Macrophages and Microglia In Vitro, Part 2: Assessing Inflammasome Activation

            Marta Lovotti, Matthew S.J. Mangan, Róisín M. McManus, Kateryna Shkarina, Matilde B. Vasconcelos, and Eicke Latz

 

30. Detection of G-Quadruplex DNA Structures in Macrophages

            Melanie Kastl, Fabian Hersperger, Katrin Kierdorf, and Katrin Paeschke

 

31. Adaptation of Human iPSC-Derived Macrophages Towards an Alveolar Macrophage-Like Phenotype Post Intra-Pulmonary Transfer into Murine Models

            Miriam Hetzel, Ingrid Gensch, Mania Ackermann, and Nico Lachmann

 

32. Tackling Tissue Macrophage Heterogeneity by SplitCre Transgenesis

            Sigalit Boura-Halfon, Rebecca Haffner-Krausz, Shifra Ben-Dor, Jung-Seok Kim, and Steffen Jung

 

33. Automated Cell Counting of Macrophages In Situ

            Ouzéna Bouadi and Tuan Leng Tay

 

34. Morphometric Analyses of Macrophages

            Jan N. Hansen

 

35. Combined Analysis of mRNA Expression and Open Chromatin in Microglia

            Rebekka Scholz, Desirée Brösamle, Xidi Yuan, Jonas J. Neher, and Marc Beyer