Tissue Morphogenesis: Methods and Protocols

ISBN-13: 9781493911639 / Angielski / Twarda / 2014 / 351 str.

Celeste M. Nelson

Opis

Tissue Morphogenesis: Methods and Protocols highlights major techniques, both experimental and computational, for the study of tissue morphogenesis, divided into several sections, with specific focus on techniques to image, manipulate, model and analyze tissue morphogenesis. Chapters focus on imagining analysis of tissue morphogenesis, culture models of tissue morphogenesis, manipulating cells and tissues in vivo, novel model systems to investigate issue morphogenesis and computational models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Tissue Morphogenesis: Methods and Protocols serves as a primary resource for both fundamental and practical understanding of the techniques used to uncover the basis of tissue morphogenesis.

Spis treści

PART I          IMAGING MORPHOGENESIS

 Chapter 1.       Probing Regional Mechanical Properties of Embryonic Tissue Using                                                Microindentation and Optical Coherence Tomography

Benjamin A. Filas, Gang Xu, and Larry A. Taber

 

Chapter 2.       Hemodynamic Flow Visualization of Early Embryonic Great Vessels using mPIV

Selda Goktas, Chia-Yuan Chen, William J. Kowalski, and Kerem Pekkan

 

Chapter 3.       Using Correlative Light and Electron Microscopy to Study Zebrafish Vascular Morphogenesis

Jacky G. Goetz, Fabien Monduc, Yannick Schwab, and Julien Vermot

 

Chapter 4.       Micro/Nano-Computed Tomography Technology for Quantitative Dynamic, Multi-scale Imaging of Morphogenesis

 Chelsea L. Gregg, Andrew K. Recknagel, and Jonathan T. Butcher

 

Chapter 5.       Imaging the Dorsal-Ventral Axis of Live and Fixed Drosophila Melanogaster Embryos

Sophia N. Carrell and Gregory T. Reeves

 

Chapter 6.       Light-Sheet-Based Imaging and Analysis of Early Embryogenesis in the Fruit Fly

Khaled Khairy, William Lemon, Fernando Amat, and Philipp J. Keller

 

Chapter 7.       Quantitative Image Analysis of Cell Behavior and Molecular Dynamics During Tissue Morphogenesis

 Chun Yin Bosco Leung and Rodrigo Fernandez-Gonzalez

 

Chapter 8.       A Multiplex Fluorescent in situ Hybridization Protocol for Clonal Analysis of Drosophila Oogenesis

 Lily S. Cheung and Stanislav Shvartsman

 

Chapter 9.       Active Cell and ECM Movements During Development

Anastasiia Aleksandrova, Brenda J. Rongish, Charles D. Little, and Andras Czirok

 

PART II         CULTURE MODELS OF MORPHOGENESIS

 Chapter 10.     3D Culture Assays of Murine Mammary Branching Morphogenesis and Epithelial Invasion

Kim-Vy Nguyen-Ngoc, Eliah R. Shamir, Robert J. Huebner, Jennifer N. Beck, Kevin J. Cheung, and Andrew J. Ewald

 Chapter 11.     Culture of Mouse Embryonic Foregut Explants

 Felicia Chen and Wellington V. Cardoso

 Chapter 12.     Investigating Human Vascular Tube Morphogenesis and maturation using  endothelial cell-pericyte co-cultures and a doxycycline-inducible genetic system in 3D extracellular matrices

 Stephanie L.K. Bowers, Chun-Xia Meng, Matthew T. Davis, and George E. Davis

 Chapter 13.     Three-Dimensional Traction Force Microscopy of Engineered Epithelial Tissues

 Alexandra S. Piotrowski, Victor D. Varner, Nikolce Gjorevski, and Celeste M. Nelson

 

PART III       MANIPULATING CELLS AND TISSUES IN VIVO

Chapter 14.     Probing Cell Mechanics with Sub-Cellular Laser Dissection of Actomyosin  Networks in the Early Developing Drosophila Embryo

 M. Rauzi and P.-F. Lenne

 

Chapter 15.     UV Laser Ablation to Measure Cell and Tissue-Generated Forces in the Zebrafish    Embryo in vivo and ex vivo

Michael Smutny, Martin Behrndt, Pedro Campinho, Verena Ruprecht, and Carl-Philipp Heisenberg

 

Chapter 16.     Measurement of Intercellular Cohesion by Tissue Surface Tension

 Ramsey A. Foty

 

Chapter 17.     Quail-Chick Chimeras and Eye Development

 Sinu Jasrapuria Agrawal and Peter Y. Lwigale

 

PART IV       EMERGING MODELS OF TISSUE MORPHOGENESIS

 Chapter 18.     Studying Epithelial Morphogenesis in Dictyostelium

 Daniel J. Dickinson, W. James Nelson, and William I. Weis

 

Chapter 19.     Primary Cell Cultures of Regenerating Holothurian Tissues

 Samir A. Bello, Ricardo J. Abreu-Irizarry, and Jose E. Garcia-Arraras

 

PART V         COMPUTATIONAL MODELS OF TISSUE MORPHOGENESIS

 Chapter 20.     Large-Scale Parameter Studies of Cell-Based Models of Tissue Morphogenesis   Using CompuCell3D or VirtualLeaf

Margriet M. Palm and Roeland M. H. Merks

 

Chapter 21.     Simulating Tissue Morphogenesis and Signaling

Dagmar Iber, Simon Tanaka, Patrick Fried, Philipp Germann, and Denis  Menshykau

 

Chapter 22.     Elasticity-Based Targeted Growth Models of Morphogenesis

 Patrick W. Alford

Blurb

Tissue Morphogenesis: Methods and Protocols highlights major techniques, both experimental and computational for the study of tissue morphogenesis. Chapters focus on imagining analysis of tissue morphogenesis, culture models of tissue morphogenesis, manipulating cells and tissues in vivo, novel model systems to investigate issue morphogenesis, and computational models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and practical, Tissue Morphogenesis: Methods and Protocols serves  as a primary resource for both fundamental and practical understanding of the techniques used to uncover the basis of tissue morphogenesis.