Tissue Microarrays: Methods and Protocols

ISBN-13: 9781607618058 / Angielski / Twarda / 2010 / 204 str.

Ronald Simon

Opis

The tissue microarray (TMA) method presents as a modern high technology, although its roots go back to the 80s when researchers frst started to combine several small pieces of tissues into so-called sausage blocks. In this respect, the TMA invention was not frstly characterized by technical improvements, but its true novelty was to link clinical data to the tissues that were combined on one slide. The very high number of tissues that can be included into one TMA, the small size and regular shape of the tissue spots, the preser- tion of integrity of the donor tissue blocks, and the highly organized array pattern that allows for reliable allocation of clinical data to individual tissue spots made it a discrete technique with unique features. When the TMA technology was developed 12 years ago, its beneft was controversially debated. While many researchers welcomed the method enthusiastically, there were c- cerns by others that results obtained from the small tissue cores used for TMA making would not be suffciently representative of the donor tissues. Meanwhile, the increasing use of this technology has imposingly demonstrated its tremendous utility in research. In fact, basically all clinically relevant associations between molecular markers and clinical endpoints could be reproduced using only one single 0. 6 mm core per tissue sample so that TMAs have nowadays become a standard tool allowing for a new dimension of tissue analysis.

Recenzje

From the reviews:

"This book is the first ... to attempt to summarise the field of Tissue Microarrays (TMAs) since the first report of the technology in 1998. ... relevant to the clinician-scientist who may be considering high-throughput analyses of genes and proteins from a large cohort. ... In summary, this book is crammed with incredibly detailed technical advice ... and even advice on whether to drill or cut the recipient paraffin blocks. ... very readable, very informative and an excellent introduction to the gentle art of TMAs." (Cris dos Remedios and Sean Lal, Proteomics, Vol. 11, 2011)

Spis treści

Tissue Microarrays: Methods and Protocols Editor Ronald Simon Table of Contents: Table of Contents Preface Contributors 1. Applications of tissue microarray technology Ronald Simon 2. Quality aspects of TMA analysis Pierre Tennstedt and Guido Sauter 3. Representativity of TMA studies Guido Sauter 4. Recipient block TMA technique Martina Mirlacher and Ronald Simon 5. Protocol for constructing tissue arrays by cutting edge matrix assembly Thai Hong Tran, Justin Lin, Ashley Brooke Sjolund, Fransiscus Eri Utama, and Hallgeir Rui 6. Hypodermic needle without recipient paraffin block technique Andréa Rodrigues Cordovil Pires and Simone Rabello de Souza 7. Resin Technologies: Constructing and staining of resin TMAs William J Howat and Susan J Wilson 8. Tissue microarrays from frozen tissues - OCT technique Marlena Schoenberg Fejzo and Dennis J. Slamon 9. An Alternative Technology to Prepare Tissue Microarray Using Frozen Tissue Samples Zhongting Hu, Elbert Chang, Melissa Hodeib, and Joseph D. Abad 10. Building “Tissue” Microarrays from Suspension Cells Shuchun Zhao and Yasodha Natkunam 11. TMAs from Biopsy specimens Milton W. Datta and André A. Kajdacsy-Balla, 12. Immunohistochemical analysis of tissue microarrays Ronald Simon, Martina Mirlacher and Guido Sauter 13. DNA copy number analysis on Tissue Microarrays Anne Kallioniemi 14. RNA expression analysis on formalin-fixed paraffin-embedded (FFPE) tissue in TMA format by RNA in situ hybridization Jürgen Veeck and Edgar Dahl 15. Automated analysis of Tissue Microarrays Marisa Dolled-Filhart, Mark Gustavson, Robert L. Camp, David L. Rimm, John L. Tonkinson, and Jason Christiansen 16. Digital microscopy for boosting database integration and analysis in TMA studies Tibor Krenacs, L. Ficsor, V. S. Varga , V. Angeli , and B. Molnar 17. From Gene to Clinic: TMA based clinical validation of molecular markers in prostate cancer T. Schlomm, F. K. Chun, and A.Erbersdobler

Blurb

Today’s tissue microarray (TMA) method presents as a modern high-tech technology, one which allows for the linking of clinical data to the tissues that are combined on one slide. In Tissue Microarrays: Methods and Protocols, expert researchers explore the current world of TMA making and TMA applications, providing insight into the inherent and complex aspects of the most popular assays used for in-situ tissue analysis. Chapters examine the range of TMA techniques that allow for a large number of tissues to be included in one TMA, preserve the integrity of donor tissue blocks, and present a highly organized array pattern that allows for the reliable allocation of clinical data to individual tissue spots. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Contemporary and ground-breaking, Tissue Microarrays: Methods and Protocols serves as an essential handbook for pathologists, molecular biologists, researchers in the life sciences, as well as physicians, a reflection of the various applications of current TMA technology.