Thyroid Hormone Nuclear Receptor: Methods and Protocols

ISBN-13: 9781493993208 / Angielski / Miękka / 2019 / 300 str.

Plateroti, Michelina

Opis

This volume aims to detail the advances in the field of Thyroid hormones (THs), namely T3 and T4, and their nuclear receptor TRs from a conceptual and methodological point of view. Chapters guide readers through a general introduction, TR's engineered mice, human genetics of TRs, control of the hypothalamus-pituitary-thyroid (HPT) axis, the relevance of the local THs production and metabolism and pathological consequences of TH/TR alterations. Non-mammalian models are also presented and discussed. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Thyroid Hormone Nuclear Receptor: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Spis treści

1. Thyroid Hormone Receptors: Several Players for One Hormone and Multiple Functions

Jacques Samarut and Michelina Plateroti

 

2. Two Protocols to Study the Interactions of Thyroid Hormone Receptors with Other Proteins and Chromatin

Wenyue Guan, Romain Guyot, and Frédéric Flamant

 

3. Expression Analysis of Genes Regulated by Thyroid Hormone in Neural Cells

Juan Bernal and Beatriz Morte

4. In vitro Approaches to Identify Thyroid Hormone Receptor-dependent Transcriptional Response

Maria Sirakov and Michelina Plateroti

 

5. Posttranslational Modification of Thyroid Hormone Nuclear Receptor by Phosphorylation

Yan-Yun Liu and Gregory A. Brent

 

6. Posttranslational Modification of Thyroid Hormone Nuclear Receptor by Sumoylation

Yan-Yun Liu and Gregory A. Brent

 

7. Demonstration of the Receptor Site for Thyroid Hormone on Integrin αvβ3

Hung-Yun Lin, Shaker A. Mousa, and Paul J Davis

 

8. The Deiodinase Trio and Thyroid Hormone Signaling

Antonio C. Bianco and Rodrigo R. da Conceição

 

9. Thyroid Hormones and Derivatives – Endogenous Thyroid Hormones and their Targets

Josef Köhrle

 

10. Protocols for Studying how Thyroid Hormone Receptors Trigger Adaptive Thermogenesis in Mice Karine Gauthier

 

11. Pharmacological Inhibition of Lysosomal Activity as a Method For Monitoring Thyroid Hormone-induced Autophagic Flux in Mammalian Cells in vitro

Jin Zhou, Rohit A. Sinha, Ronny Lesmana, Winifred WY. Yau, and Paul M. Yen

 

12. Analysis of Physiological Responses to Thyroid Hormones and their Receptors in Bone

J.H. Duncan Bassett and Graham R. Williams

 

13. Evaluating the Hypothalamic-Pituitary-Thyroid (HPT) Axis in Mice

FE Wondisford

 

14. Thyroid Hormone Action: The p43 Mitochondrial Pathway

Chantal Wrutniak-Cabello, François Casas, and Gérard Cabello

 

15. Evaluating thyroid Disrupting Chemicals in vivo using Xenopus laevis

Bilal B. Mughal, Barbara A Demeneix, and Jean-Baptiste Fini

 

16. Quantification of Thyromimetic Sobetirome Concentration in Biological Tissue Samples

Jordan Devereaux, Skylar J. Ferrara, and Thomas S. Scanlan

 

17. Analysis of Thyroid Tumorigenesis in Xenograft Mouse Model

Xuguang Zhu and Sheue-yann Cheng

 

18. Human Genetics Of Thyroid Hormone Receptor Beta: Resistance to thyroid Hormone Beta

Theodora Pappa and Samuel Refetoff

 

19. TRa Mutations in Human

Briet C, Bouhours-Nouet Natacha, Illouz F, Prunier-Mirebeau D, and  Rodien P

 

20. Role of Thyroid Hormone Receptor in Amphibian Development

Liezhen Fu, Luan Wen, and Yun-Bo Shi

 

21. de novo Transcriptomic Approach To Study Thyroid Hormone Receptor Action in Non-mammalian Models

Nicolas Buisine, Gwenneg Kerdivel, and Laurent M. Sachs

 

22. Role of TRs in Zebrafish Development

Federica Marelli and Luca Persani