Therapeutic Antibodies: Methods and Protocols

ISBN-13: 9781071614495 / Angielski / Twarda / 2021 / 347 str.

Gunnar Houen

Spis treści

1. Therapeutic Antibodies: An Overview

            Gunnar Houen

 

2. Treatment of Autoimmune Diseases with Therapeutic Antibodies: Lessons Learned from PID Patients Allow for Stratification of the Infection Risk

            Joyce J.B.C. van Beers and Jan G.M.C. Damoiseaux

 

3. B-Cell Analysis for Monitoring Patients Undergoing B-Cell Depletion for the Treatment of Autoimmune Diseases

            Jan G.M.C. Damoiseaux, Joyce van Beers, Matthias Busch, and Pieter van Paassen

 

4. Assessment of Therapeutic Antibody Developability by Combinations of In Vitro and In Silico Methods

            Adriana-Michelle Wolf Perez, Nikolai Lorenzen, Michele Vendruscolo, and Pietro Sormanni

 

5. The Therapeutic Antibody Profiler for Computational Developability Assessment

            Matthew I.J. Raybould and Charlotte M. Deane

 

6. Short Read-Length Next-Generation DNA Sequencing of Antibody CDR Combinations from Phage Selection Outputs

            Landon Pastushok, Kris Barreto, and C. Ronald Geyer

 

7. Large Scale Transient Production in ExpiCHO-S^TM with Enhanced N-Galactosylation-Sialylation and PEI-Based Transfection

            Xiaotian Zhong, Ashley Schwab, Weijun Ma, Caryl L. Meade, Jing Zhou, Aaron M. D’ Antona, Will Somers, and Laura Lin

 

8. Production of Therapeutic Single-Chain Variable Fragments (ScFv) in Pichia pastoris

            Laia Montoliu-Gaya and Sandra Villegas

 

9. Purification of Therapeutic Antibodies by Protein A Affinity Chromatography

            Anurag S. Rathore and Sharad Narnaware

 

10. Ion Exchange Chromatographic Methods for Purification of Therapeutic Antibodies

            Anurag S. Rathore and Vishwanath Hebbi

 

11. Structure-Indicated LC-MS/MS Bioanalysis of Therapeutic Antibodies

            Noriko Iwamoto and Takashi Shimada

 

12. Use of PASEF for Accelerated Protein Sequence Confirmation and De Novo Sequencing with High Data Quality

            Detlev Suckau, Waltraud Evers, Eckhard Belau, Stuart Pengelley, Anja Resemann, Wilfred Tang, K. Ilker Sen, Elsa Wagner, Olivier Colas, and Alain Beck

 

13. Nano-Microscopy of Therapeutic Antibody Aggregates in Solution

            Yukako Senga, Toshihiko Ogura, Hiroshi Imamura, and Shinya Honda

 

14. Measuring Self-Association of Antibody Lead Candidates with Dynamic Light Scattering

            Fabian Dingfelder, Anette Henriksen, Per-Olof Wahlund, Paolo Arosio, and Nikolai Lorenzen

 

15. Intact Mass Quantitation of Therapeutic Antibodies for Pharmacokinetic Studies Using Immuno-Purification

            Lisa A. Vasicek, Daniel S. Spellman, and Kevin P. Bateman

 

16. Detection of Residual Host Cell Proteins in Biotherapeutic Drugs by Concatenated 2D LC-MS/MS

            Feng Yang, Delia Li, and Donald E. Walker

 

17. Bioassays for the Evaluation of Target Neutralization and Complement-Dependent Cytotoxicity (CDC) of Therapeutic Antibodies

            Nohemí Salinas-Jazmín, Emilio Medina-Rivero, and Marco Antonio Velasco-Velázquez

 

18. Methods for Functional Characterization of FcRn Interactions with Therapeutic Antibodies and Fc-Fusion Proteins

            Shan Chung, Yuwen L. Lin, Van Nguyen, and Chang Liu

 

19. Method for Measurement of Antibody-Dependent Cellular Phagocytosis

            Lynn Kamen, Benjamin Ordonia, Srividya Myneni, and Shan Chung

 

20. Bioluminescent Bridging Immunoassay for Anti-Drug Antibody (ADA) Detection

            Nidhi Nath, Becky Godat, Rod Flemming, and Marjeta Urh

 

21. A Surface Plasmon Resonance-Based Assay for Simultaneous Measurement of Serum Concentrations of Therapeutic Antibodies and Anti-Drug Antibodies

            Marten Beeg, Eleonora Allocati, and Marco Gobbi

Blurb

This detailed book covers methods for studying, producing, and analyzing therapeutic antibodies, measuring their concentration, developing neutralizing antibodies for them, and for predicting and monitoring their therapeutic efficacy and clinical effects. These biologics are the fastest growing pharmaceutical drug group and have had tremendous clinical and scientific impact in cancer, autoimmune diseases, infectious diseases, and other immune-related diseases, making the content of this volume essential. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible methods, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Therapeutic Antibodies: Methods and Protocols serves as an ideal guide for researchers working with the production of, research on, and development of therapeutic antibodies as well as for clinicians using therapeutic antibodies in daily work with patients.