The Tumor Microenvironment: Methods and Protocols

ISBN-13: 9781493937998 / Angielski / Twarda / 2016 / 356 str.

Josie Ursini-Siegel; Nicole Beauchemin

Opis

This book covers core and emerging in vitro and in vivo protocols used to study how various components of the tumor microenvironment are established and subsequently interact with tumor cells to facilitate carcinogenesis. In addition, the book examines research topics including cellular and molecular biology approaches, in vivo genetic approaches, various omics -based strategies, therapeutic strategies to target the microenvironment, and, finally, advanced techniques in the fields of tissue engineering and nanotechnology. Written and validated in the laboratories of a number of trusted collaborating authors for the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, The Tumor Microenvironment: Methods and Protocols constitutes a compendium of techniques now available to a broad audience, including basic and clinician scientists, systems biologists, and biological engineers."

Spis treści

1. Methods of Immunohistochemistry and Immunofluorescence: Converting Invisible to Visible

Hidetoshi Mori and Robert D. Cardiff

 

2. Laser Capture Microdissection as a Tool to Study Tumor Stroma

Nicholas R. Bertos and Morag Park

 

3. Quantitative Analysis of Human Cancer Cell Extravasation Using Intravital Imaging

Lian Willetts, David Bond, Konstantin Stoletov, and John D. Lewis

 

4. Studies on the Tumor Vasculature and Coagulant Microenvironment

Esterina D’Asti, Brian Meehan, and Janusz Rak

 

5. A Microfluidic Method to Mimic Luminal Structures in the Tumor Microenvironment

José A. Jiménez-Torres, David J. Beebe, and Kyung E. Sung

 

6. Measuring Vascular Permeability In Vivo

Eelco F.J. Meijer, James W. Baish, Timothy P. Padera, and Dai Fukumura

 

7. Hydroxylation-Dependent Interaction of Substrates to the Von Hippel-Lindau Tumor Suppressor Protein (VHL)

Pardeep Heir and Michael Ohh

 

8. Analyzing the Tumor Microenvironment by Flow Cytometry

Yoon Kow Young, Alicia M. Bolt, Ryuhjin Ahn, and Koren K. Mann

 

9. Detecting Secreted Analytes from Immune Cells: An Overview of Technologies

Kelly A. Pike, Caitlyn Hui, and Connie M. Krawczyk

 

10. Purification of Immune Cell Populations from Freshly Isolated Murine Tumors and Organs by Consecutive Magnetic Cell Sorting and Multi-Parameter Flow Cytometry-Based Sorting

Camilla Salvagno and Karin E. de Visser

 

11. Viral Engineering of Chimeric Antigen Receptor Expression on Murine and Human T Lymphocytes

Joanne A. Hammill, Arya Afsahi, Jonathan L. Bramson, and Christopher W. Helsen

 

12. Methods to Evaluate the Anti-Tumor Activity of Immune Checkpoint Inhibitors in Preclinical Studies

Bertrand Allard, David Allard, and John Stagg

 

13. Isolation and Characterization of Low Vs. High-Density Neutrophils in Cancer

Jitka Y. Sagiv, Sandra Voels, and Zvi Granot

 

14. Analysis of Extracellular Vesicles in the Tumor Microenvironment

Khalid Al-Nedawi and Jolene Read

 

15. Visualizing the Tumor Microenvironment of Liver Metastasis by Spinning Disc Confocal Microscopy

Liane Babes and Paul Kubes

 

16. Intravital Microscopy for Imaging the Tumor Microenvironment in Live Mice

Victor Naumenko, Craig Jenne, and Douglas J. Mahoney

 

17. Development of a Patient-Derived Xenograft Model Using Brain Tumor Stem Cell Systems to Study Cancer

Chirayu Chokshi, Manvir Dhillon, Nicole McFarlane, Chitra Venugopal, and Sheila K. Singh

 

18. Modeling Breast Tumor Development with a Humanized Mouse Model

Lisa M. Arendt

 

19. CRISPR/Cas9 Genome Editing as a Strategy to Study the Tumor Microenvironment in Transgenic Mice

Yojiro Yamanaka

 

20. Metabolomics Analyses of Cancer Cells in Controlled Microenvironments

Simon-Pierre Gravel, Daina Avizonis, and Julie St-Pierre

 

21. Analysis of the Tumor Microenvironment Transcriptome Via NanoString mRNA and miRNA Expression Profiling

Marie-Noël M’Boutchou and Léon C. van Kempen

 

22. RNA-Seq as a Tool to Study the Tumor Microenvironment

Pudchalaluck Panichnantakul, Mathieu Bourgey, Alexandre Montpetit, Guillaume Bourque, and Yasser Riazalhosseini

 

23. Sample Preparation for Mass Spectrometry Analysis of Protein-Protein Interactions in Cancer Cell Lines and Tissues

Alice Beigbeder, Lauriane Vélot, D. Andrew James, and Nicolas Bisson