The Tumor Microenvironment: Methods and Protocols

ISBN-13: 9781071629130 / Angielski / Twarda / 2023 / 414 str.

Josie Ursini-Siegel

Opis

This second edition provides update and new chapters detailing core and emerging in vitro and in vivo protocols.  Chapters guide readers through cellular and molecular biology approaches, in vivo genetic approaches, various “omics”-based strategies, therapeutic strategies, and advanced techniques in the fields of tissue engineering and nanotechnology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, The Tumor Microenvironment: Methods and Protocols, Second Edition is a valuable resource for both novice and expert scientists in this developing field.

Spis treści

1. Imaging Mass Cytometry in Immuno-oncology

Tiziana Cotechini, Oliver Jones, and Charles Colin Thomas Hindmarch

 

2. Analyzing the Tumor Immune Microenvironment by Flow Cytometry

François Santinon, Yoon Kow Young, Sonia Victoria del Rincon, and Koren K. Mann

 

3. Blood sample processing and banking for functional and molecular analyses

Paméla Thébault, Jean-François Cailhier, and Réjean Lapointe

 

4. Quantitatively Assessing the Respiratory Burst in Innate Immune Cells

Ava L. Sargent, Jordan A. Leedberg, Jessica E. Burress, Purva S. Dalwadi, Kevin S. O’Fallon, Erin Gaffney-Stomberg, and Peter C.W. Gaines

 

5. Visualizing NETosis using a Novel Neutrophil Extracellular Trap-Specific Marker

Meghan L. De Meo, Muhammad H. Shahzad, and Jonathan D. Spicer

 

6. In vitro methods to evaluate macrophage polarization and function in cancer

Aldo Ummarino, Clément Anfray, Akihiro Maeda, Fernando Torres Andón, and Paola Allavena

 

7. In vivo analysis of tumor associated macrophages in the tumor microenvironment

Clément Anfray, Aldo Ummarino, Alfonso Calvo, Paola Allavena, and Fernando Torres Andón

 

8. Assessing the immune regulatory role of metabolites in vitro

Sarah McPhedran, Junu Choe, Sarah MacPherson, Gabrielle Kowalchuk, Vanessa Chan, Marisa K. Kilgour, and Julian J. Lum

 

9. Mouse Models of Obesity To Study The Tumor Immune Microenvironment

Sarah Petrecca and Daniela F Quail

 

10. Mouse Models of Obesity To Study The Tumor Immune Microenvironment

Sarah Petrecca and Daniela F Quail

 

11. Assessing the efficacy of immune checkpoint inhibitors in pre-clinical tumor models

Celia Jacoberger-Foissac, Bertrand Allard, David Allard, and John Stagg

 

12. Fibroblast isolation from mammary gland tissue and syngeneic murine breast cancer models

Margarita Bartish, Julian Smith-Voudouris, and Sonia del Rincón

  13. Multiscale label-free imaging of fibrillar collagen in the tumor microenvironment

Michael S. Nelson, Yuming Liu, Helen Wilson, Bin Li, Ivan M Rosado-Mendez, Jeremy D. Rogers, Walter F. Block, and Kevin W. Eliceiri

 

14. In situ measurement of intra-tumoral tissue rigidity

Benjamin E. Campbell, Stephanie Mok, and Christopher Moraes

 

15. Extracellular matrix glycation and crosslinking in mammary tumor progression

Jason J. Northey

 

16. Proteomic assessment of the murine mammary gland extracellular matrix

Samuel E. J. Preston, Vincent R. Richard, Sonia V. del Rincón, Christoph H. Borchers, and René P. Zahedi

 

17. Spheroids as a 3D Model of the Hypoxic Tumor Microenvironment

Sarah M. Kirsh, Sydney A. Pascetta, and James Uniacke

 

18. Tumor pH imaging using Chemical Exchange Saturation Transfer (CEST)-MRI

Dario Livio Longo, Elisa Pirotta, Riccardo Gambino, Feriel Romdhane, Antonella Carella, and Alessia Corrado

 

19. Profiling the epigenetic landscape of the tumor microenvironment using chromatin immunoprecipitation-sequencing

Marina Fukano, Gabriel Alzial, Raphaëlle Lambert, and Geneviève Deblois

 

20. Analysis of Spatial Molecular Data

Alona Levy-Jurgenson, Xavier Tekpli, Vessela N. Kristensen, and Zohar Yakhini

 

21. Laser-assisted single-cell labeling and capture

Nicolas Desjardins-Lecavalier , Graziana Modica, and Santiago Costantino

 

22. 3D hydrogel cultures for high-throughput drug discovery

Karen Sperle, Darrin J. Pochan, and Sigrid A. Langhans

 

23. Intravital microscopy of the metastatic pulmonary environment

Liane Babes

 

24. A CRISPR platform for targeted in vivo screens

Vincent Maranda, Yue Zhang, Frederick S. Vizeacoumar, Andrew Freywald, and Franco J. Vizeacoumar