The Plant Endoplasmic Reticulum: Methods and Protocols

ISBN-13: 9781493973880 / Angielski / Twarda / 2017 / 265 str.

Chris Hawes; Verena Kriechbaumer

Opis

This volume presents a range of different techniques that have been used to characterize the structure and function of the endoplasmic reticulum (ER) in higher plants. Chapters guide readers through application of modern microscopy techniques by fluorescence and electron microscopy, new protocols for analysing ER network structure, methods to purify and analyse ER membrane structure and to study protein glycosylation, protocols to study the unfolded protein response, and the role of the ER in autophagy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Plant Endoplasmic Reticulum: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Spis treści

1. Labelling the ER for Light and Fluorescence Microscopy

Chris Hawes, Pengwei Wang, and Verena Kriechbaumer

 

2. 3D Electron Microscopy of the ER

Maike Kittelmann

 

3. Characterization of Proteins Localised to Plant ER-PM Contact Sites

Pengwei Wang, Chris Hawes, and Patrick J. Hussey

 

4. Preparation and Imaging of Specialised ER using Super-resolution and TEM Techniques

Karen Bell, Karl Oparka and Kirsten Knox

 

5. Quantitation of ER Structure and Function

Mark Fricker, Luke Heaton, Nick Jones, Boguslaw Obara, Stefanie J. Müller, and Andreas J. Meyer

 

6. Long-term Imaging of Endoplasmic Reticulum Morphology in Embryos during Seed Germination

Natasha Dzimitrowicz, Emily Breeze, and Lorenzo Frigerio

 

7. Dancing with the Stars: Using Image Analysis to Study the Choreography of the Endoplasmic Reticulum and its Partners and of Movement within its Tubules

Lawrence R. Griffing

 

8. Preparation of Highly Enriched ER Membranes using Free-flow Electrophoresis

Harriet T. Parsons

 

9. ER Microsome Preparation in Arabidopsis thaliana

Verena Kriechbaumer

 

10. ER Membrane Lipid Composition and Metabolism: Lipidomic Analysis

Laetitia Fouillen, Lilly Maneta-Peyret, and Patrick Moreau

 

11. 2in1 Vectors Improve in Planta BiFC and FRET Analysis

Dietmar Mehlhorn, Niklas Wallmeroth, Kenneth W. Berendzen, and Christopher Grefen

 

12. Metabolons on the Plant ER

Verena Kriechbaumer and Stanley W. Botchway

 

13. Using Optical Tweezers Combined with Total Internal Reflection Microscopy to Study Interactions between the ER and Golgi in Plant Cells

Imogen Sparkes, Rhiannon R White, Benjamin Coles, Stanley Botchway, and Andy Ward

 

14. Protein Biosynthesis and Maturation in the ER

Emanuela Pedrazzini and Alessandro Vitale

 

15. ER Membrane Protein Interactions using the Split-Ubiquitin System (SUS)

Lisa Yasmin Asseck, Niklas Wallmeroth, and Christopher Grefen

 

16. Analysis of Protein Glycosylation in the ER

Jennifer Schoberer, Yun-Ji Shin, Ulrike Vavra, Christiane Veit, and Richard Strasser

 

17. The Unfolded Protein Response

Kazuki Tabara, Yuji Iwata, and Nozomu Koizumi

 

18. Unfolded Protein Response in Arabidopsis

Cristina Ruberti and Federica Brandizzi

 

19. Fluorescence Imaging of Autophagy-mediated Er-to-vacuole Trafficking in Plants

Hadas Peled-Zehavi and Gad Galili

 

20. Imaging the ER and Endomembrane System in Cereal Endosperm

Verena Ibl, Jenny Peters, Eva Stoger, and Elsa Arcalís