The Plant Cytoskeleton: Methods and Protocols

ISBN-13: 9781071628669 / Angielski / Twarda / 2023 / 369 str.

Patrick J. Hussey; Pengwei Wang

Opis

This detailed volume explores the development of technologies and protocols that are currently being used to understand the nature and activities of the plant cytoskeleton. A focus for many of the chapters is on sample preparation, as the quality of plant organ/tissue preparation, from single to multicellular samples, determines the quality of the data. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, The Plant Cytoskeleton: Methods and Protocols serves as an ideal guide for researchers interested in or starting to be interested in plant cell and molecular biology research.

Spis treści

1. Quantification of Microtubule Bundling Activity of MAPs Using TIRF Microscopy

            Sharol Schmidt-Marcec, Austin Ross, and Andrei Smertenko

 

2. Actin: Static and Dynamic Studies

            Huaqiang Ruan, Sha Zhang, Yi Zhang, and Haiyun Ren

 

3. 3D Visualization of Microtubules in Epidermal Pavement Cells

            Amir J. Bidhendi, Bara Altartouri, and Anja Geitmann

 

4. Quantitative Analysis of Microtubule Organization in Leaf Epidermis Pavement Cells

            Sandra Klemm, Jonas Buhl, Birgit Möller, and Katharina Bürstenbinder

 

5. Single Cell Confinement Methods to Study Plant Cytoskeleton

            Pauline Durand-Smet, Antoine Chevallier, Léia Colin, Alice Malivert, Isaty Melogno, and Olivier Hamant

 

6. Documentation of Microtubule Collisions with Myosin VIII ATM1 Containing Membrane-Associated Structures

            Eduard Belausov, Vikas Dwivedi, Sela Yechezkel, Sefi Bar-Sinai, and Einat Sadot

 

7. Imaging the Plant Cytoskeleton by High-Pressure Freezing and Electron Tomography

            Janice Pennington and Marisa S. Otegui

 

8. Confocal Microscopic Assays of Mitotically Active Proteins in an Agrobacterial Infiltration-Based, Cell Division-Enabled Leaf System of Tobacco (Nicotiana benthamiana)

            Yuh-Ru Julie Lee, Calvin H. Huang, and Bo Liu

 

9. Assessment of Spindle Shape Control by Spindle Poleward Flux Measurements and FRAP Bulk Analysis

            Sabine Müller

 

10. Expansion Microscopy of Plant Cells (PlantExM)

            Timothy J. Hawkins, Joanne L. Robson, Bethany Cole, and Simon J. Bush

 

11. Microfluidic Device for High-Resolution Cytoskeleton Imaging and Wash Out Assays in Physcomitrium (Physcomitrella) patens

            Mari W. Yoshida and Elena Kozgunova

 

12. Using Spinning Disk Microscopy to Observe the Mitotic and Cytokinetic Apparatus in Physcomitrium patens

            Yuji Hiwatashi and Takashi Murata

 

13. Gaining Insight into Large Gene Families with the Aid of Bioinformatic Tools

            Fatima Cvrčková and Radek Bezvoda

 

14. Cell-to-Cell Connectivity Assays for the Analysis of Cytoskeletal and Other Regulators of Plasmodesmata

            Zoe Barr and Jens Tilsner

 

15. Studying Nuclear Dynamics in Response to Actin Disruption In Planta

            Joseph F. McKenna and Katja Graumann

 

16. Cytoskeleton Remodelling in Arabidopsis Stigmatic Cells Following Pollination

            Lucie Riglet and Isabelle Fobis-Loisy

 

17. Investigation of ROP GTPase Activity and Cytoskeleton Dynamics during Tip Growth in Root Hairs and Pollen Tubes

            Lei Zhu and Ying Fu

 

18. Functional Analysis of Phospholipid Signaling and Actin Dynamics: The Use of Apical Growing Tobacco Pollen Tubes in a Case Study

            Teresa Braga, Fernando Vaz Dias, Marta Fratini, Susana Serrazina, Ingo Heilmann, and Rui Malhó

 

19. Microtubule Reorganization during ABA-Induced Stomatal Closure in Arabidopsis

            Liru Dou, Xiangfeng Wang, and Tonglin Mao

 

20. Imaging of Cortical Microtubules in Plants under Salt Stress

            Shuwei Wang, Liyuan Xu, Changjiang Li, Lei Zhu, Ying Fu, and Yan Guo

 

21. Analysis of Actin Array Rearrangement during the Plant Response to Bacterial Stimuli

            Bingxiao Wang, Minxia Zou, Qing Pan, and Jiejie Li

 

22. Live-Cell Imaging of Cytoskeletal Responses and Trafficking during Fungal Elicitation

            Amber J. Connerton, Stefan Sassmann, and Michael J. Deeks

 

23. Visualization and Quantification of the Dynamics of Actin Filaments in Arabidopsis Pollen Tubes

            Qiaonan Lu, Xiaonan Liu, Xiaolu Qu, and Shanjin Huang

 

24. Non-Invasive Long-Term Imaging of the Cytoskeleton in Arabidopsis Seedlings

            Felix Ruhnow, Staffan Persson, and René Schneider

 

25. Visualization of Cytoskeleton Organization and Dynamics in Elongating Cotton Fibers by Live-Cell Imaging

            Guangda Wang, Yanjun Yu, and Zhaosheng Kong

 

26. Methods to Visualize and Quantify Cortical Microtubule Arrays in Arabidopsis Conical Cells

            Xie Dang, Lilan Zhu, Huibo Ren, and Deshu Lin

 

27. Studying the Organization of the Actin Cytoskeleton in the Multicellular Trichomes of Tomato

            Zhijing Xu, Xiaolu Qu, Shuang Wu, and Pengwei Wang

 

28. Light Microscopy Technologies and the Plant Cytoskeleton

            Timothy J. Hawkins

 

29. Investigating Plant Protein-Protein Interactions Using FRET-FLIM with a Focus on the Actin Cytoskeleton

            Patrick Duckney and Patrick J. Hussey