The Plant Cell Wall: Methods and Protocols

ISBN-13: 9781617790072 / Angielski / Twarda / 2011 / 326 str.

Zoe A. Popper

Opis

The cell wall and its constituent polysaccharides and proteins control nearly all plant-based biological and biophysical processes. Understanding the cell wall is, therefore, not only fundamental to the plant sciences but is also pertinent to aspects of human and animal nutrition and health as well as plant-microbe and plant-animal interactions. In The Plant Cell Wall: Methods and Protocols, experts in the field describe detailed methods which are currently being applied to investigate the many aspects of the plant cell wall including its structure, biochemical composition, and metabolism. The book delves into a range of techniques involving plant tissue culture, which can be applied to investigating cell wall structure and metabolism, methods directed towards structural analysis and occurrence of carbohydrates, the development and use of microscopy-based tools and techniques, procedures which measure the physical properties of the wall, and methods based on the application of molecular genetic approaches. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and easily accessible, The Plant Cell Wall: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this essential cellular feature.

Spis treści

1. Plant Tissue Cultures

            Anna Kärkönen, Arja Santanen, Kuninori Iwamoto, and Hiroo Fukuda

 

2. Computerized Molecular Modelling of Carbohydrates

            Alfred D. French and Glenn P. Johnson

 

3. Oligosaccharide Mass Profiling (OLIMP) of Cell Wall Polysaccharides by MALDI-TOF/MS

            Markus Günl, Florian Kraemer, and Markus Pauly

 

4. High-Voltage Paper Electrophoresis (HVPE) of Cell-Wall Building Blocks and Their Metabolic Precursors

            Stephen C. Fry

 

5. Carbohydrate Gel Electrophoresis

            Florence Goubet, Paul Dupree, and Katja Salomon Johansen

 

6. Capillary Electrophoresis with Detection by Laser-Induced Fluorescence

            Andrew Mort and Xiangmei Wu

 

7. Monoclonal antibodies, Carbohydrate-Binding Modules, and the Detection of Polysaccharides in Plant Cell Walls

            Cécile Hervé, Susan E. Marcus, and J. Paul Knox

 

8. Screening and Characterization of Plant Cell Walls Using Carbohydrate Microarrays

            Iben Sørensen and William G.T. Willats

 

9. Electron Tomography and Immunogold Labelling as Tools to Analyze de novo Assembly of Plant Cell Walls

            Marisa S. Otegui

 

10. Analyzing Cellulose Biosynthesis with Confocal Microscopy

            Meera Nair and Seth DeBolt

 

11. Visual Mapping of Cell Wall Biosynthesis

            Yumiko Sakuragi, Morten H.H. Nørholm, and Henrik V. Scheller

 

12. Atomic Force Microscopy of Plant Cell Walls

            Andrew R. Kirby

 

13. Using Solid-State ¹³C NMR Spectroscopy to Study the Molecular Organization of Primary Plant Cell Walls

            Tracey J. Bootten, Philip J. Harris, Laurence D. Melton, and Roger H. Newman

 

14. Formation of Cellulose-Based Composites with Hemicelluloses and Pectins Using Gluconacetobacter Fermentation

            Deirdre Mikkelsen and Michael J. Gidley

 

15. Structural Proteins of the Primary Cell Wall: Extraction, Purification, and Analysis

            Derek T.A. Lamport, Li Tan, and Marcia J. Kieliszewski

 

16. New Insights into the Control of Cell Growth

            Claudia Blaukopf, Matthäus Z. Krol, and Georg J. Seifert

 

17. Extraction and Detection of Arabinogalactan Proteins

            Zoë A. Popper

 

18. Characterization of the Plant Cell Wall Proteome Using High Throughput Screens

            Sang-Jik Lee and Jocelyn K.C. Rose

 

19. Knocking Out the Wall: Protocols for Gene Targeting in Physcomitrella patens

            Alison W. Roberts, Christos S. Dimos, Michael J. Budziszek, Jr., Chessa A. Goss, and Virginia Lai

 

20. Measuring In vitro Extensibility of Growing Plant Cell Walls

            Daniel J. Cosgrove