The Nuclear Receptor Superfamily: Methods and Protocols

ISBN-13: 9781493937226 / Angielski / Twarda / 2016 / 250 str.

Iain J. McEwan

Opis

This volume aims to describe a complementary range of molecular, cell biological, and in vivo protocols used to investigate the structure-function of nuclear receptors, together with experimental approaches that may lead to new drugs to selectively target nuclear receptor-associated diseases. The Nuclear Receptor Superfamily, Second Edition will benefit those starting out in the nuclear receptor research field as well as to more established researchers who wish to apply different methods to a particular receptor or research problem. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Nuclear Receptor Superfamily, Second Edition aims to ensure successful results in the further study of this vital field.

Spis treści

The Nuclear Receptor Superfamily- At Thirty.- Lipid Homeostasis and Ligands for Liver X Receptors: Identification and Characterization.- Use of Differential Scanning Fluorimetry to Identify Nuclear Receptor Ligands.- Drug-Discovery Pipeline for Novel Inhibitors of the Androgen Receptor.- Use of BRET to Study Protein-Protein Interactions in vitro and in vivo.- Studying Steroid Receptor Complexes in the Cellular Environment.- Post-translational Modifications of Steroid Receptors: Phosphorylation.- Mapping protein-DNA Interactions using ChIP-exo and Illumina-based Sequencing.- Methods to Identify Chromatin Bound Protein Complexes: From Genome-Wide to Locus Specific Approaches.- Measuring the Expression of microRNAs Regulated by Androgens.- Methods for Identifying and Quantifying Mrna Expression of Androgen Receptor Splicing Variants in Prostate Cancer.- Harvesting Human Prostate Tissue Material and Culturing Primary Prostate Epithelial Cells.- In vivo Imaging of Nuclear Receptor Transcriptional Activity.- Development and Characterisation of Cell-Specific Androgen Receptor Knockout Mice.